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The map label for this gene is nrdE

Identifier: 54020061

GI number: 54020061

Start: 188455

End: 190617

Strand: Direct

Name: nrdE

Synonym: mhp158

Alternate gene names: 54020061

Gene position: 188455-190617 (Clockwise)

Preceding gene: 54020060

Following gene: 54020062

Centisome position: 21.11

GC content: 31.02

Gene sequence:

>2163_bases
ATGAAAAAAATGGGTTTGGATCTGAATTTTGATAAAAATCAGGAAAAAAGTTATCTTAGCTTAAATGCGAATAGCAAAAT
TTTTACAAATCATCCTGATTCACATAAATTTGATTTATTAGCAGCAGAAAAATATATTAATGAAGAAATTAATCCAAAAT
TTAAATTTTTCAATTCTTTTAAAGAAAGAATTGAATTTTTAGTTCAAAATAATTATTATGATCCGAAAGTTTTAGGACAA
TATTCATTCCAAGAACTTGAGAAATTAAATACAAAAGCTTGAAGTTATAATCATTTTTTTTCTTCTTTTATGGGGGCTTT
TAAATTTTATTCAACTTATGGACTAAAATCAAATGATAATTTAGTATATTTTGAGCATTTTCCCGATCGGGTTTTACTAA
ATAGTTTATTTTTAGGAAAAGGAGATTTTCAAAAAGCTGAAAAAATTCTTGAACAAATTATGTTAGGTCGATTTCAACCC
GCAACCCCGACATTTTTAAATGCCGGAAAATTAAACAGAGGTGAATTTGTCTCTTGTTATTTGATCCGAGTTGAAGATAA
TATGGAATCAATCTCCAGGGCAATTACTACTTCACTCCAACTTTCAAAACGTGGCGGTGGTGTTAGTGTTTTACTTTCAA
ATTTGCGCGAACAAGGTGCCCCGATTAAAAACATTGAAAATCAAGCAACTGGTGTAATTCCGGTAATGAAAATTCTTGAA
GATTCATTTTCTTATGCCAACCAGTTAGGGCAACGTCAAGGTGCTGGGGCTGTTTATTTAAATGTCCACCATCCGGATAT
TCTGGCTTTTTTAGATACCAAAAGGGAAAATGCTGATGAAAAAATCAGGATAAAATCGCTTTCTCTTGGGATAATAATTC
CGAATATTACTTTTGAATTAGCAAAAAATAATGAAAAAATGGCGCTTTTTTCTGTCTATGATGTTGAAAAAGTATATCAA
AAAGCTTTTAGTGATATTTCTATTAGTGAAAAATATTATCAAATGCTAGCAAATCCAGAAATTAAAAAAACATTTATTTC
GGCAAGAAAGTTATTTAAAATTATTGCCGAGCTACATTTTGAAAGTGGTTATCCTTATATTTTATTTGAAGATATTGTCA
ATAAAGAAAATGCTCATCCTGATCGCGTTGTAATGTCAAATTTATGTAGCGAAATCACACAGCCTTCAACTCCAAGCACA
TTTTTACCCGATCTAAGTTTTAAAGAAATAGGCAAGGATATTTGTTGTAATTTAGGATCGCTAAATATTGATAAAGCGAT
GGAATCAGGCCCTGATTTTGAACAAATGGTTTATTATTCAGTGTTAGCTCTTGATGTTGTCTCGCGAAATTCTGATCTTT
CAGTAGCCCCTTCAATTCAAAAAGGAAATAATTTAAATCATGCAATTGGTCTTGGGGCGATGAATTTACACGGGTTTTTG
GCTAAAAATGAAATTTTTTATGACTCAGAGGAAGCGCTTGATTTTACTAACATTTTTTTCTATACTTTAGCTTTTTCAGC
TTTTAAAGCCTCTGAAAAACTTGCAAAAAAATATGGTCCTTTTTCTGGATTTGAAAAAACCAAATTTGCATCAGGAGAAT
ATTTTGATAAATATACAAAAGTTTCCCCGAATACTTTTTTACCAAAAACACATAAAATTAAAGAACTTTTTGCAAAATAT
CAAGTTGAAATTCCGACTCAAGAAGATTGAATTCATCTTGTTGAAAAAATTAAAAAAACAGGAATTGCCAACTCGCATCT
AATGGCGATTGCTCCTACAGGCTCAATTTCCTATCTTAGTTCCTGCACGCCTTCGCTTCAGCCAGTTGTTGCCCCAGTTG
AGATAAGAAAAGAAGGAAAATTAGGTAGAATTTATGTTCCTTCATATAAATTAAGTCCTAAAACATACAAATTTTATCAA
AACGGAGCTTATGAATTAGGTCCAATTCCGATTATAAACATTGTCTCAGAAGCACAAAAACATGTTGATCAAGCAATCTC
GATGACCTTATTTATGACCGACAAGGCGACTACTCGAGACTTAAATCGAGCTTATATTTATGCTTTTAAAAAAAATTGTA
AGTCAATTTACTATGTAAGAATTCGTCAAGATGTCCTTGAGGATTCAGAAAATTATGAAACTTGTCAATCTTGCCAGATT
TAA

Upstream 100 bases:

>100_bases
TGTAAAAAAGGTTCGGCAAATTTTAGAAGATTTTTGAGGAAAATAAGCTAAAAAATAATGAATATACAGGTAGTTCGCTG
ACTAAATTGGGAGAAAATAA

Downstream 100 bases:

>100_bases
AACAAACTAAAAAATACTTAAATTTAAAGTATTTTTACTAAATAAAGGTTAAAAGTATAACTAATTTATATAAGGTACTA
AAGATAAATTCCTTGTTTTC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MKKMGLDLNFDKNQEKSYLSLNANSKIFTNHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFLVQNNYYDPKVLGQ
YSFQELEKLNTKAWSYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQP
ATPTFLNAGKLNRGEFVSCYLIRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILE
DSFSYANQLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMALFSVYDVEKVYQ
KAFSDISISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPST
FLPDLSFKEIGKDICCNLGSLNIDKAMESGPDFEQMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFL
AKNEIFYDSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFLPKTHKIKELFAKY
QVEIPTQEDWIHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQ
NGAYELGPIPIINIVSEAQKHVDQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI

Sequences:

>Translated_720_residues
MKKMGLDLNFDKNQEKSYLSLNANSKIFTNHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFLVQNNYYDPKVLGQ
YSFQELEKLNTKA*SYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQP
ATPTFLNAGKLNRGEFVSCYLIRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILE
DSFSYANQLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMALFSVYDVEKVYQ
KAFSDISISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPST
FLPDLSFKEIGKDICCNLGSLNIDKAMESGPDFEQMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFL
AKNEIFYDSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFLPKTHKIKELFAKY
QVEIPTQED*IHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQ
NGAYELGPIPIINIVSEAQKHVDQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI
>Mature_720_residues
MKKMGLDLNFDKNQEKSYLSLNANSKIFTNHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFLVQNNYYDPKVLGQ
YSFQELEKLNTKA*SYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQP
ATPTFLNAGKLNRGEFVSCYLIRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILE
DSFSYANQLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMALFSVYDVEKVYQ
KAFSDISISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPST
FLPDLSFKEIGKDICCNLGSLNIDKAMESGPDFEQMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFL
AKNEIFYDSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFLPKTHKIKELFAKY
QVEIPTQED*IHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQ
NGAYELGPIPIINIVSEAQKHVDQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=657, Percent_Identity=24.0487062404871, Blast_Score=162, Evalue=8e-40,
Organism=Escherichia coli, GI1789030, Length=684, Percent_Identity=52.7777777777778, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=588, Percent_Identity=26.3605442176871, Blast_Score=163, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17555242, Length=661, Percent_Identity=24.6596066565809, Blast_Score=162, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6320914, Length=668, Percent_Identity=25.1497005988024, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322123, Length=667, Percent_Identity=24.7376311844078, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17352481, Length=618, Percent_Identity=23.9482200647249, Blast_Score=153, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81619; Mature: 81619

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMGLDLNFDKNQEKSYLSLNANSKIFTNHPDSHKFDLLAAEKYINEEINPKFKFFNSF
CCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
KERIEFLVQNNYYDPKVLGQYSFQELEKLNTKASYNHFFSSFMGAFKFYSTYGLKSNDNL
HHHHHHHHHCCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQPATPTFLNAGKLNRGEFVSCYL
EEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEEEE
IRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILED
EEEECCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
SFSYANQLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELA
HHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCEEEEE
KNNEKMALFSVYDVEKVYQKAFSDISISEKYYQMLANPEIKKTFISARKLFKIIAELHFE
CCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
SGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPSTFLPDLSFKEIGKDICCNLGSL
CCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHCCCCCC
NIDKAMESGPDFEQMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFLA
CHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHCCCCCHHHHEEE
KNEIFYDSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKV
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCC
SPNTFLPKTHKIKELFAKYQVEIPTQEDIHLVEKIKKTGIANSHLMAIAPTGSISYLSSC
CCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHC
TPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQNGAYELGPIPIINIVSEAQKHV
CCCCCCEECCCEECCCCCCCEEEECCEECCCCEEEHHCCCCEECCCCHHHHHHHHHHHHH
DQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI
HHHHEEEEEEECCCHHHCCCHHEEEEEHHCCCEEEEEEEEHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MKKMGLDLNFDKNQEKSYLSLNANSKIFTNHPDSHKFDLLAAEKYINEEINPKFKFFNSF
CCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
KERIEFLVQNNYYDPKVLGQYSFQELEKLNTKASYNHFFSSFMGAFKFYSTYGLKSNDNL
HHHHHHHHHCCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQPATPTFLNAGKLNRGEFVSCYL
EEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEEEE
IRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILED
EEEECCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
SFSYANQLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELA
HHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCEEEEE
KNNEKMALFSVYDVEKVYQKAFSDISISEKYYQMLANPEIKKTFISARKLFKIIAELHFE
CCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
SGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPSTFLPDLSFKEIGKDICCNLGSL
CCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHCCCCCC
NIDKAMESGPDFEQMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFLA
CHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHCCCCCHHHHEEE
KNEIFYDSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKV
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCC
SPNTFLPKTHKIKELFAKYQVEIPTQEDIHLVEKIKKTGIANSHLMAIAPTGSISYLSSC
CCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHC
TPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQNGAYELGPIPIINIVSEAQKHV
CCCCCCEECCCEECCCCCCCEEEECCEECCCCEEEHHCCCCEECCCCHHHHHHHHHHHHH
DQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI
HHHHEEEEEEECCCHHHCCCHHEEEEEHHCCCEEEEEEEEHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]