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The map label for this gene is glyA

Identifier: 54020057

GI number: 54020057

Start: 184972

End: 186228

Strand: Direct

Name: glyA

Synonym: mhp154

Alternate gene names: 54020057

Gene position: 184972-186228 (Clockwise)

Preceding gene: 54020056

Following gene: 54020058

Centisome position: 20.72

GC content: 33.97

Gene sequence:

>1257_bases
ATGTATAAGAAAATCAAACTTAGGGACCAACAAATTTCTGAGCTGATTAATTTAGAATCTAAAAGACAGAATAGCCAAAT
CGAGTTAATTGCAAGTGAAAATTATGCCTCAGAAGACGTAATTTTGGCAAATGGAACAAGTCTTAGTAACAAATATGGTG
AAGGTTATCCGGGGAAAAGGTATTATGGTGGCTGTACTTTTATCGATCAAATTGAAAAAATCGCAATTGAAAGAGTAAAA
AAACTTTTTAAGATTGAGTATGCAAATGTTCAACCTTATTCTGGATCAAGTGCAAACGCCGCTGTTTTTGCAGCACTTTT
AAAGCCCGGGGATAAAATTCTTGGACTTGACTTAAATGCTGGTGGGCATTTAAGTCATGGATATAAAATCAATTTTTCAG
GAATGTTTTATTCAGGAATTAGTTATTTTTTAGATGAAAATGAACTTCTTGATTATGACGCGATCGAAAAAATAGCCTTA
AAAACAAAGCCAAATTTAATTATTTGTGGTTATTCAGCATATTCAAGAAAAATTGATTTTGCCCGTTTTCGACAGATCGC
TGATAAAGTAAATGCGTTTTTACTTGCTGATATTGCTCATATTGCCGGGCTTATTGCTGCAGGCCAACATCCTTCGCCGG
TTGGATATGCCCATATAATAACCTCAACAACCCAGAAAACTTTGAGAGGACCAAGAGGCGGCCTGATTTTAACTAACAGT
AAAGAAATTGCTGCAAAAATTGATAAAGTTGTCTTTCCGGGAATTCAAGGCGGGCCTTTTTTTCATACAATTGCGGCGAA
AGCTGTTGCTTTTAAAGAAGCTTTAGAACCTTGATTTAAAGAATATTGTGCCCAAATTGTTAAAAATGCATCGCATTTTG
CTAGTGAATTTATAAAAAAAGGAATAAGAATTGTATCTCAGGGTACCGAAAACCACCTTTTTACCATCGACGTTCTTAGT
TCTTATAATCTAAATGGCAAACAGGCGCAAATTTTACTTGAATCTGTTAATATTATCACCAATAAAAACACAATTCCAAA
TGATACTTTAAGTCCATTTGTAACTTCGGGATTACGTTTGGGAACCCCGGCGATGACTTCGCGGGGCTTTAAAGAACAGG
AATTTAGTCAAATGGCAGAAATAATTGATTTTGTTCTGAGAAAAAAAGAGCTAAATGCGCTTGAAATTAAAGAAATTAAG
AAAAAAGTTAAAATTTTAACTAAAAATTTCCCGATTAAAAAAAGTTACTGACCTTAA

Upstream 100 bases:

>100_bases
TGTTTGCGAACAAAAAAGTAACGTGTTTTAAAGGTTTTTTATAAAAAAAATTAAAAAAATTATTTTTTTGGTTTTTTAGT
GTATAATGTATAAAATTCCA

Downstream 100 bases:

>100_bases
ACGTTAAAAAAGTCTATAAATTATGTTATTTTTTTAACTTATGGTTTATAAATATAAAATTATTTTTATTTCTTATTCTG
AAAAAACAAAAAAAGGTTAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MYKKIKLRDQQISELINLESKRQNSQIELIASENYASEDVILANGTSLSNKYGEGYPGKRYYGGCTFIDQIEKIAIERVK
KLFKIEYANVQPYSGSSANAAVFAALLKPGDKILGLDLNAGGHLSHGYKINFSGMFYSGISYFLDENELLDYDAIEKIAL
KTKPNLIICGYSAYSRKIDFARFRQIADKVNAFLLADIAHIAGLIAAGQHPSPVGYAHIITSTTQKTLRGPRGGLILTNS
KEIAAKIDKVVFPGIQGGPFFHTIAAKAVAFKEALEPWFKEYCAQIVKNASHFASEFIKKGIRIVSQGTENHLFTIDVLS
SYNLNGKQAQILLESVNIITNKNTIPNDTLSPFVTSGLRLGTPAMTSRGFKEQEFSQMAEIIDFVLRKKELNALEIKEIK
KKVKILTKNFPIKKSYWP

Sequences:

>Translated_418_residues
MYKKIKLRDQQISELINLESKRQNSQIELIASENYASEDVILANGTSLSNKYGEGYPGKRYYGGCTFIDQIEKIAIERVK
KLFKIEYANVQPYSGSSANAAVFAALLKPGDKILGLDLNAGGHLSHGYKINFSGMFYSGISYFLDENELLDYDAIEKIAL
KTKPNLIICGYSAYSRKIDFARFRQIADKVNAFLLADIAHIAGLIAAGQHPSPVGYAHIITSTTQKTLRGPRGGLILTNS
KEIAAKIDKVVFPGIQGGPFFHTIAAKAVAFKEALEP*FKEYCAQIVKNASHFASEFIKKGIRIVSQGTENHLFTIDVLS
SYNLNGKQAQILLESVNIITNKNTIPNDTLSPFVTSGLRLGTPAMTSRGFKEQEFSQMAEIIDFVLRKKELNALEIKEIK
KKVKILTKNFPIKKSY*P
>Mature_418_residues
MYKKIKLRDQQISELINLESKRQNSQIELIASENYASEDVILANGTSLSNKYGEGYPGKRYYGGCTFIDQIEKIAIERVK
KLFKIEYANVQPYSGSSANAAVFAALLKPGDKILGLDLNAGGHLSHGYKINFSGMFYSGISYFLDENELLDYDAIEKIAL
KTKPNLIICGYSAYSRKIDFARFRQIADKVNAFLLADIAHIAGLIAAGQHPSPVGYAHIITSTTQKTLRGPRGGLILTNS
KEIAAKIDKVVFPGIQGGPFFHTIAAKAVAFKEALEP*FKEYCAQIVKNASHFASEFIKKGIRIVSQGTENHLFTIDVLS
SYNLNGKQAQILLESVNIITNKNTIPNDTLSPFVTSGLRLGTPAMTSRGFKEQEFSQMAEIIDFVLRKKELNALEIKEIK
KKVKILTKNFPIKKSY*P

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=449, Percent_Identity=42.7616926503341, Blast_Score=345, Evalue=6e-95,
Organism=Homo sapiens, GI261862352, Length=449, Percent_Identity=42.7616926503341, Blast_Score=344, Evalue=9e-95,
Organism=Homo sapiens, GI261862350, Length=449, Percent_Identity=42.7616926503341, Blast_Score=344, Evalue=9e-95,
Organism=Homo sapiens, GI261862348, Length=449, Percent_Identity=42.7616926503341, Blast_Score=344, Evalue=9e-95,
Organism=Homo sapiens, GI261862346, Length=448, Percent_Identity=42.1875, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI22547186, Length=406, Percent_Identity=44.3349753694581, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI22547189, Length=392, Percent_Identity=42.3469387755102, Blast_Score=280, Evalue=3e-75,
Organism=Escherichia coli, GI1788902, Length=406, Percent_Identity=51.7241379310345, Blast_Score=413, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25144729, Length=431, Percent_Identity=43.1554524361949, Blast_Score=338, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI25144732, Length=430, Percent_Identity=43.2558139534884, Blast_Score=337, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=418, Percent_Identity=44.4976076555024, Blast_Score=342, Evalue=5e-95,
Organism=Saccharomyces cerevisiae, GI6323087, Length=426, Percent_Identity=43.1924882629108, Blast_Score=325, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24640005, Length=450, Percent_Identity=41.7777777777778, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI221329721, Length=450, Percent_Identity=41.7777777777778, Blast_Score=347, Evalue=9e-96,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46099; Mature: 46099

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKKIKLRDQQISELINLESKRQNSQIELIASENYASEDVILANGTSLSNKYGEGYPGKR
CCCCCCCCHHHHHHHHCCHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCC
YYGGCTFIDQIEKIAIERVKKLFKIEYANVQPYSGSSANAAVFAALLKPGDKILGLDLNA
EECCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECC
GGHLSHGYKINFSGMFYSGISYFLDENELLDYDAIEKIALKTKPNLIICGYSAYSRKIDF
CCCCCCCEEEEECCEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCHH
ARFRQIADKVNAFLLADIAHIAGLIAAGQHPSPVGYAHIITSTTQKTLRGPRGGLILTNS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEECC
KEIAAKIDKVVFPGIQGGPFFHTIAAKAVAFKEALEPFKEYCAQIVKNASHFASEFIKKG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRIVSQGTENHLFTIDVLSSYNLNGKQAQILLESVNIITNKNTIPNDTLSPFVTSGLRLG
HHEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHCCCCCC
TPAMTSRGFKEQEFSQMAEIIDFVLRKKELNALEIKEIKKKVKILTKNFPIKKSYP
CCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYKKIKLRDQQISELINLESKRQNSQIELIASENYASEDVILANGTSLSNKYGEGYPGKR
CCCCCCCCHHHHHHHHCCHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCC
YYGGCTFIDQIEKIAIERVKKLFKIEYANVQPYSGSSANAAVFAALLKPGDKILGLDLNA
EECCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECC
GGHLSHGYKINFSGMFYSGISYFLDENELLDYDAIEKIALKTKPNLIICGYSAYSRKIDF
CCCCCCCEEEEECCEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCHH
ARFRQIADKVNAFLLADIAHIAGLIAAGQHPSPVGYAHIITSTTQKTLRGPRGGLILTNS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEECC
KEIAAKIDKVVFPGIQGGPFFHTIAAKAVAFKEALEPFKEYCAQIVKNASHFASEFIKKG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRIVSQGTENHLFTIDVLSSYNLNGKQAQILLESVNIITNKNTIPNDTLSPFVTSGLRLG
HHEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHCCCCCC
TPAMTSRGFKEQEFSQMAEIIDFVLRKKELNALEIKEIKKKVKILTKNFPIKKSYP
CCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA