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The map label for this gene is lplA

Identifier: 54020046

GI number: 54020046

Start: 145867

End: 146877

Strand: Direct

Name: lplA

Synonym: mhp125

Alternate gene names: 54020046

Gene position: 145867-146877 (Clockwise)

Preceding gene: 54020045

Following gene: 54020047

Centisome position: 16.34

GC content: 27.6

Gene sequence:

>1011_bases
TTGGAGATAATTATGAAAATTTATACAACAAAAAATACAAGCCCGTTTTATACACTAGTCTGTGAAGAAATTATTCTAAA
AGATGAAGAAAACCAAGAGGATATTCTTTACTTCTACCAACATAAAAATGCGATAATTATTGGAAAAAATCAAAATATTT
ACGAAGAAATTAAACTCGAGGAAGTTGAAGAGGAAAATATTGAAATCTACCGTCGGCTTTCAGGGGGTGGGGCGGTCTAT
CATGATCTAGGTAATATAAATTTTTCCTTTATTAGTCAAAAGCAAAATCATAATTATCAAAAATTTCTATCACCAATTAT
CGAATTTTTTCGATCCTTAGGGCTAAAAGCTGAATTTAAGGGACGAAATGATTTAATTGTCAATGGCGCAAAAGTTTCGG
GTAATGCCCAGATAATTTTTAAAAATAAAATTGTTCATCATGGAACAATTTTATTTAATGCTAATTTAGCAAAATTAGCA
CAAGTTTTAAAGCCAAGTCGACTAAAAATTGAATCAAAAGGAATTAAATCTGTCCGCCAACGAGTAACAAACATTCTCTA
TGAAATGGAAGAGCAAATTGATGATAATGAATTTATTTACCGTTTAATTTCATTTTTTGAAAAAAAATATCAAACAAAAT
CAGTTGATGTTGAAAATCATTTTGATATAGGAATTTCAAATACTGAAAAATTCAAGGAAATAAGTCAATTACGAGAATCA
AAAAACTGAATTTTTGGAACCAACCCATTTTTTAGTTATGAAAATATCAAACGAACAAGTGCCGGAATTTTGAAGGTCTT
AGCAAATATTGAAAAAAATATAATTATAAAAATTAAGTTTGAAGGTGATTTTTTATCACAAAGGCCAATTGAAGAAATAG
AAGAAATTTTGGAAAATAAAGAATTTGACCGTGATAGTCTTGAGAGAGAACTAGAAAAAATTCATAATTTAAATGAGTAT
TTTGGTCAAATTCTACCTTATGAAATTTTAGATACAATTTTTGGAGTTTAA

Upstream 100 bases:

>100_bases
ATTTTTAATTTAGAAAATTTGCGACTAGGCGAAAATCTAGCCGATTTTAAATCAAAATTTAAACTAATTACGACTTTTAA
TTTAGAATATAACTAAAATT

Downstream 100 bases:

>100_bases
ATGGTAGTAAATCGAAAAATTAAAATTGAAAATGAAGAAATTTTCTACTTTTTAGAAGATAATAATCGACCAAAAGTACT
TTTTCTCCATGGTTTTAACT

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MEIIMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVEEENIEIYRRLSGGGAVY
HDLGNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLA
QVLKPSRLKIESKGIKSVRQRVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRES
KNWIFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDRDSLERELEKIHNLNEY
FGQILPYEILDTIFGV

Sequences:

>Translated_336_residues
MEIIMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVEEENIEIYRRLSGGGAVY
HDLGNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLA
QVLKPSRLKIESKGIKSVRQRVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRES
KN*IFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDRDSLERELEKIHNLNEY
FGQILPYEILDTIFGV
>Mature_336_residues
MEIIMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLEEVEEENIEIYRRLSGGGAVY
HDLGNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFKGRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLA
QVLKPSRLKIESKGIKSVRQRVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRES
KN*IFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKEFDRDSLERELEKIHNLNEY
FGQILPYEILDTIFGV

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729884, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729882, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729880, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21729878, Length=254, Percent_Identity=29.1338582677165, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1790846, Length=314, Percent_Identity=31.5286624203822, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71981909, Length=223, Percent_Identity=30.9417040358744, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322415, Length=293, Percent_Identity=31.3993174061433, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28573464, Length=187, Percent_Identity=31.0160427807487, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 39244; Mature: 39244

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLE
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHH
EVEEENIEIYRRLSGGGAVYHDLGNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFK
HHHHCCHHHHEEECCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEC
GRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLAQVLKPSRLKIESKGIKSVRQ
CCCCEEEECEEECCCEEEEEECCEEECEEEEEECCHHHHHHHHCCCCEEECHHHHHHHHH
RVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRES
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KNIFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKE
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
FDRDSLERELEKIHNLNEYFGQILPYEILDTIFGV
CCHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MEIIMKIYTTKNTSPFYTLVCEEIILKDEENQEDILYFYQHKNAIIIGKNQNIYEEIKLE
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHH
EVEEENIEIYRRLSGGGAVYHDLGNINFSFISQKQNHNYQKFLSPIIEFFRSLGLKAEFK
HHHHCCHHHHEEECCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEC
GRNDLIVNGAKVSGNAQIIFKNKIVHHGTILFNANLAKLAQVLKPSRLKIESKGIKSVRQ
CCCCEEEECEEECCCEEEEEECCEEECEEEEEECCHHHHHHHHCCCCEEECHHHHHHHHH
RVTNILYEMEEQIDDNEFIYRLISFFEKKYQTKSVDVENHFDIGISNTEKFKEISQLRES
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KNIFGTNPFFSYENIKRTSAGILKVLANIEKNIIIKIKFEGDFLSQRPIEEIEEILENKE
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
FDRDSLERELEKIHNLNEYFGQILPYEILDTIFGV
CCHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]