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The map label for this gene is lacY [H]

Identifier: 52787856

GI number: 52787856

Start: 4006956

End: 4008197

Strand: Reverse

Name: lacY [H]

Synonym: BLi04179

Alternate gene names: 52787856

Gene position: 4008197-4006956 (Counterclockwise)

Preceding gene: 52787857

Following gene: 52787855

Centisome position: 94.92

GC content: 42.19

Gene sequence:

>1242_bases
ATGAAAATGAAAAGCTCAAACAGTCTGTATTGGAAACTAAGCGCCTATTTTTTCTTTTTCTTTTTTACTTGGTCTTCCAG
CTATTCTTTATTTGCGATTTGGTTAGGGCAAGAAATCAATTTGAACGGGTCCGCGACGGGCATTATCTTTTCTGTAAACG
CTATCTTTACTTTGTGCATGCAGCCTTTGTACGGTTTTATCTCCGATAAGCTCGGGCTGAAGAAAAACATATTATTTATG
ATCAGTTTGCTGCTCGTATTTACGGGTCCCTTTTATATTTTCGTCTACGGACCGCTTTTGCAATACAACGTCTTTCTTGG
GGCTATCGTCGGGGGAATTTATTTGGGAACTGCTTTTCTCGCCGGAATCGGTGCGATTGAAACCTTTATTGAAAAGGTCA
GCCGCAAATATCAATTTGAATATGGAAGAACAAGGATGTGGGGGTCCCTCGGCTGGGCTGCGGCGACATTTTTTGCAGGT
CAGCTGTTCAATATCGATCCGAATATCAACTTCTGGGTTGCGTCCGCCTCAGCAATCATATTGGTGGCCATTATTGTTTC
CGTAAAAATTGAGATGACAGATGATGAAAAGGAAAGAGCAGACTCGGTCGGATTAAAAGACGTAGGAGGGCTTTTTCTCT
TAAAAGATTTCTGGTTTTTGATGCTGTACGTGATCGGCGTAACATGCGTGTACGGCGTCTATGACCAGCAGTTCCCGCTT
TACTACGCTTCCTTATTTCCGACTGCGGCCTTGGGGAACCAAATATTTGGATACCTTAATTCATTCCAAGTATTTATTGA
AGCGGGCATGATGTTTCTTGCGCCTTTCATCGTCAATAAGCTCGGTCCTAAAAAAAGCTTGATTTTAGCGGGGCTGTTAA
TGGCTTTCCGGATTATCGGTTCCGGACTTGTCAGCGGACCGGTCGGAATTTCATCGATGAAACTCATTCATGCTTTAGAA
TTGCCGATTATGCTGATTGCGATGTTTAAATATTTGGCGACTAATTTTGATAATCGTCTTTCATCCGTACTTTATCTTGT
CGGCTTTCAATTCGCATCCCAGGTAGGCACGTCGATTTTTTCGCCGCTTGCGGGAGGTTTATACGACAGCATCGGATTTC
GCCACACTTATCTCATCATGGGAGCAATGGTCCTTTGTTTTACCATTATTTCGATTTTTACCTTGCTAGACTCAAAGAAA
GATGTTGAATTTGCTCAAAATCTACAAAGCAATCATATATAG

Upstream 100 bases:

>100_bases
ATTCTGATCTACATTGTAGCCCAACGCTACATTTTAGATGGCCTGGGCGGTTCAGGGACAGGCATCAAATAGATACATCC
AAATCGGGTTAAAGGAGCAA

Downstream 100 bases:

>100_bases
AGAGGATGTCACAGATGAACAGAATTCAGCAGGCAGAAGAAGCATTAAAGAAAGCCGGGAAAAAAGTGAATCGCCGTTAC
CGAATGGGCTATCACATGAT

Product: galactoside permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Lactose-proton symport [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKMKSSNSLYWKLSAYFFFFFFTWSSSYSLFAIWLGQEINLNGSATGIIFSVNAIFTLCMQPLYGFISDKLGLKKNILFM
ISLLLVFTGPFYIFVYGPLLQYNVFLGAIVGGIYLGTAFLAGIGAIETFIEKVSRKYQFEYGRTRMWGSLGWAAATFFAG
QLFNIDPNINFWVASASAIILVAIIVSVKIEMTDDEKERADSVGLKDVGGLFLLKDFWFLMLYVIGVTCVYGVYDQQFPL
YYASLFPTAALGNQIFGYLNSFQVFIEAGMMFLAPFIVNKLGPKKSLILAGLLMAFRIIGSGLVSGPVGISSMKLIHALE
LPIMLIAMFKYLATNFDNRLSSVLYLVGFQFASQVGTSIFSPLAGGLYDSIGFRHTYLIMGAMVLCFTIISIFTLLDSKK
DVEFAQNLQSNHI

Sequences:

>Translated_413_residues
MKMKSSNSLYWKLSAYFFFFFFTWSSSYSLFAIWLGQEINLNGSATGIIFSVNAIFTLCMQPLYGFISDKLGLKKNILFM
ISLLLVFTGPFYIFVYGPLLQYNVFLGAIVGGIYLGTAFLAGIGAIETFIEKVSRKYQFEYGRTRMWGSLGWAAATFFAG
QLFNIDPNINFWVASASAIILVAIIVSVKIEMTDDEKERADSVGLKDVGGLFLLKDFWFLMLYVIGVTCVYGVYDQQFPL
YYASLFPTAALGNQIFGYLNSFQVFIEAGMMFLAPFIVNKLGPKKSLILAGLLMAFRIIGSGLVSGPVGISSMKLIHALE
LPIMLIAMFKYLATNFDNRLSSVLYLVGFQFASQVGTSIFSPLAGGLYDSIGFRHTYLIMGAMVLCFTIISIFTLLDSKK
DVEFAQNLQSNHI
>Mature_413_residues
MKMKSSNSLYWKLSAYFFFFFFTWSSSYSLFAIWLGQEINLNGSATGIIFSVNAIFTLCMQPLYGFISDKLGLKKNILFM
ISLLLVFTGPFYIFVYGPLLQYNVFLGAIVGGIYLGTAFLAGIGAIETFIEKVSRKYQFEYGRTRMWGSLGWAAATFFAG
QLFNIDPNINFWVASASAIILVAIIVSVKIEMTDDEKERADSVGLKDVGGLFLLKDFWFLMLYVIGVTCVYGVYDQQFPL
YYASLFPTAALGNQIFGYLNSFQVFIEAGMMFLAPFIVNKLGPKKSLILAGLLMAFRIIGSGLVSGPVGISSMKLIHALE
LPIMLIAMFKYLATNFDNRLSSVLYLVGFQFASQVGTSIFSPLAGGLYDSIGFRHTYLIMGAMVLCFTIISIFTLLDSKK
DVEFAQNLQSNHI

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=375, Percent_Identity=44, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 45998; Mature: 45998

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKSSNSLYWKLSAYFFFFFFTWSSSYSLFAIWLGQEINLNGSATGIIFSVNAIFTLCM
CCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCEEEEEHHHHHHHHH
QPLYGFISDKLGLKKNILFMISLLLVFTGPFYIFVYGPLLQYNVFLGAIVGGIYLGTAFL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AGIGAIETFIEKVSRKYQFEYGRTRMWGSLGWAAATFFAGQLFNIDPNINFWVASASAII
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEHHHHHH
LVAIIVSVKIEMTDDEKERADSVGLKDVGGLFLLKDFWFLMLYVIGVTCVYGVYDQQFPL
HHHHHHHHEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YYASLFPTAALGNQIFGYLNSFQVFIEAGMMFLAPFIVNKLGPKKSLILAGLLMAFRIIG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SGLVSGPVGISSMKLIHALELPIMLIAMFKYLATNFDNRLSSVLYLVGFQFASQVGTSIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
SPLAGGLYDSIGFRHTYLIMGAMVLCFTIISIFTLLDSKKDVEFAQNLQSNHI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKMKSSNSLYWKLSAYFFFFFFTWSSSYSLFAIWLGQEINLNGSATGIIFSVNAIFTLCM
CCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCEEEEEHHHHHHHHH
QPLYGFISDKLGLKKNILFMISLLLVFTGPFYIFVYGPLLQYNVFLGAIVGGIYLGTAFL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AGIGAIETFIEKVSRKYQFEYGRTRMWGSLGWAAATFFAGQLFNIDPNINFWVASASAII
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEHHHHHH
LVAIIVSVKIEMTDDEKERADSVGLKDVGGLFLLKDFWFLMLYVIGVTCVYGVYDQQFPL
HHHHHHHHEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YYASLFPTAALGNQIFGYLNSFQVFIEAGMMFLAPFIVNKLGPKKSLILAGLLMAFRIIG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SGLVSGPVGISSMKLIHALELPIMLIAMFKYLATNFDNRLSSVLYLVGFQFASQVGTSIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
SPLAGGLYDSIGFRHTYLIMGAMVLCFTIISIFTLLDSKKDVEFAQNLQSNHI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3056524 [H]