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The map label for this gene is gmuD [H]

Identifier: 52787845

GI number: 52787845

Start: 3995336

End: 3996772

Strand: Reverse

Name: gmuD [H]

Synonym: BLi04168

Alternate gene names: 52787845

Gene position: 3996772-3995336 (Counterclockwise)

Preceding gene: 52787846

Following gene: 52787838

Centisome position: 94.65

GC content: 47.6

Gene sequence:

>1437_bases
ATGGCCAGACAAACGTGGAAGATTCCCGACGATTTTATTCTCGGCGCAGCAGCGTCGGCGTGGCAGACAGAGGGGTGGGA
CGGGAAGCGGCCGACACAGGATTCCTATTTGGATATGTGGTACAAAAATGATCCTGATGTATGGCATGACGGATACGGAC
CGGCTGTGGCAACCGATTTTTATAACCGCTACAAAGAAGATATTCAATATATGAAAGACATCGGGCTAACCCACTACCGG
ACATCCATTAACTGGTCGCGGTTTCTTTTGGATTATGAAACCGCCGAAGTTGACGAGGTGTATGCAGGCTACATTGATGA
CGTCATCGATGAGCTGAAGAGATGCGGAGTAGAACCGATGCTTTGTCTTGAGCACTACGAAATCCCGACTGTCCTGCTGG
AACAGTATGGCGGATGGGGATCAAAACACGTGGTTGGCTTATTTGCGGCTTATGCAGAAAAAGCGTTTGAACGGTATGGG
GACAGGGTGAAATACTGGTTTACCTTTAATGAACCGATCGTGCCGCAAACACGGATCTACCTTGACGCACTTCGCTATCC
GTACGAGCAGAACACGAAAAAATGGATGCAGTGGAACTACCATAAAGCACTGGCGACAGCAAAGGCCGTGGCAATTTTCC
GGAAAAGGAAGCCCAGTTTTAGAGAAGGCGCAAAAATCGGCGTCATCTTAAATCCTGAGGTCGCGTATGCGAGGTCAAGC
GCGCCCCATGATCAGAAAGCGGCCCAAGTTTATGATTTGTTTTTTAACAGGGTTTTTTTAGATCCGAGCATAAAAGGCGT
CTATCCCGATGAATTGATGGAGCTCATGGCCAAACACGACATCCTTTTTGACCATGATGAAGAAGAGCTTTCCGTCATCA
AGCGGAATACGGTCGATTTTGTCGGTGTGAACCTGTATTATCCGAAGAGAGTTCAAGCGCCTTCAAGGCAATGGAATGAA
TCGACGCCTTTTCATCCTGCTTATTACTATGAGCCCTTTGAGCTGCCAGGCAGAAAGATGAATCCGTTCAGAGGCTGGGA
GATCTGCCCGCGCATTGTGTACGATATGGCTATGAGACTTAAATATGAGTATGGAAATATCGAATGGCTGATTGCTGAAA
ACGGCATGGGCGTCGAACATGAAGAGCGTTTTAAAAATGAAGAGGGGGTCATTCAGGACGACTACAGAATCGATTTTATT
TCAGCCCATTTGCGAGAGGCGATGAAAGGGATCGCCGACGGCGCAAACTGCAAAGGATATATGCTTTGGGCATTCACGGA
CAACGTCTCTCCGATGAACGCCTTTAAAAACCGCTACGGCCTTGTCGAGATTCGATTGGAGAACAACCGCAGCAGGGCGC
TGAAAAAATCGGCTTGCTTTTATCGGGATATCATCAAAAACAGACGGTTTGAAACAGAGGAATTCCGGTATAAATAA

Upstream 100 bases:

>100_bases
GTCCTTATTTATCTTCCGTTTGTTATTTTATTGAATCGGACCTTTACGATTAGTTCTGAAAAAAATGTGTCTATGTAAAG
CAAAGAAAGGTGTGAATGAG

Downstream 100 bases:

>100_bases
AAACGGCGGGGAATCATCCCGCTTGTCGCCGGGATGATTTCTCATATATGCCTACATAAAATATTCCGGCCGATTGATCA
TGATCTGCTTGTTCATTTGA

Product: hypothetical protein

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MARQTWKIPDDFILGAAASAWQTEGWDGKRPTQDSYLDMWYKNDPDVWHDGYGPAVATDFYNRYKEDIQYMKDIGLTHYR
TSINWSRFLLDYETAEVDEVYAGYIDDVIDELKRCGVEPMLCLEHYEIPTVLLEQYGGWGSKHVVGLFAAYAEKAFERYG
DRVKYWFTFNEPIVPQTRIYLDALRYPYEQNTKKWMQWNYHKALATAKAVAIFRKRKPSFREGAKIGVILNPEVAYARSS
APHDQKAAQVYDLFFNRVFLDPSIKGVYPDELMELMAKHDILFDHDEEELSVIKRNTVDFVGVNLYYPKRVQAPSRQWNE
STPFHPAYYYEPFELPGRKMNPFRGWEICPRIVYDMAMRLKYEYGNIEWLIAENGMGVEHEERFKNEEGVIQDDYRIDFI
SAHLREAMKGIADGANCKGYMLWAFTDNVSPMNAFKNRYGLVEIRLENNRSRALKKSACFYRDIIKNRRFETEEFRYK

Sequences:

>Translated_478_residues
MARQTWKIPDDFILGAAASAWQTEGWDGKRPTQDSYLDMWYKNDPDVWHDGYGPAVATDFYNRYKEDIQYMKDIGLTHYR
TSINWSRFLLDYETAEVDEVYAGYIDDVIDELKRCGVEPMLCLEHYEIPTVLLEQYGGWGSKHVVGLFAAYAEKAFERYG
DRVKYWFTFNEPIVPQTRIYLDALRYPYEQNTKKWMQWNYHKALATAKAVAIFRKRKPSFREGAKIGVILNPEVAYARSS
APHDQKAAQVYDLFFNRVFLDPSIKGVYPDELMELMAKHDILFDHDEEELSVIKRNTVDFVGVNLYYPKRVQAPSRQWNE
STPFHPAYYYEPFELPGRKMNPFRGWEICPRIVYDMAMRLKYEYGNIEWLIAENGMGVEHEERFKNEEGVIQDDYRIDFI
SAHLREAMKGIADGANCKGYMLWAFTDNVSPMNAFKNRYGLVEIRLENNRSRALKKSACFYRDIIKNRRFETEEFRYK
>Mature_477_residues
ARQTWKIPDDFILGAAASAWQTEGWDGKRPTQDSYLDMWYKNDPDVWHDGYGPAVATDFYNRYKEDIQYMKDIGLTHYRT
SINWSRFLLDYETAEVDEVYAGYIDDVIDELKRCGVEPMLCLEHYEIPTVLLEQYGGWGSKHVVGLFAAYAEKAFERYGD
RVKYWFTFNEPIVPQTRIYLDALRYPYEQNTKKWMQWNYHKALATAKAVAIFRKRKPSFREGAKIGVILNPEVAYARSSA
PHDQKAAQVYDLFFNRVFLDPSIKGVYPDELMELMAKHDILFDHDEEELSVIKRNTVDFVGVNLYYPKRVQAPSRQWNES
TPFHPAYYYEPFELPGRKMNPFRGWEICPRIVYDMAMRLKYEYGNIEWLIAENGMGVEHEERFKNEEGVIQDDYRIDFIS
AHLREAMKGIADGANCKGYMLWAFTDNVSPMNAFKNRYGLVEIRLENNRSRALKKSACFYRDIIKNRRFETEEFRYK

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=490, Percent_Identity=28.3673469387755, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI110681710, Length=492, Percent_Identity=27.2357723577236, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI13273313, Length=491, Percent_Identity=28.9205702647658, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI24497614, Length=490, Percent_Identity=25.3061224489796, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28376633, Length=485, Percent_Identity=25.1546391752577, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI2367174, Length=494, Percent_Identity=30.7692307692308, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI2367270, Length=483, Percent_Identity=28.9855072463768, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1789070, Length=480, Percent_Identity=28.75, Blast_Score=168, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17552856, Length=484, Percent_Identity=27.4793388429752, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17539390, Length=486, Percent_Identity=27.7777777777778, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21356577, Length=488, Percent_Identity=26.2295081967213, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 56479; Mature: 56348

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQTWKIPDDFILGAAASAWQTEGWDGKRPTQDSYLDMWYKNDPDVWHDGYGPAVATDF
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHH
YNRYKEDIQYMKDIGLTHYRTSINWSRFLLDYETAEVDEVYAGYIDDVIDELKRCGVEPM
HHHHHHHHHHHHHCCCEEEECCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LCLEHYEIPTVLLEQYGGWGSKHVVGLFAAYAEKAFERYGDRVKYWFTFNEPIVPQTRIY
EHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHH
LDALRYPYEQNTKKWMQWNYHKALATAKAVAIFRKRKPSFREGAKIGVILNPEVAYARSS
HHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCC
APHDQKAAQVYDLFFNRVFLDPSIKGVYPDELMELMAKHDILFDHDEEELSVIKRNTVDF
CCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHCCEEE
VGVNLYYPKRVQAPSRQWNESTPFHPAYYYEPFELPGRKMNPFRGWEICPRIVYDMAMRL
EEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHE
KYEYGNIEWLIAENGMGVEHEERFKNEEGVIQDDYRIDFISAHLREAMKGIADGANCKGY
EEECCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEE
MLWAFTDNVSPMNAFKNRYGLVEIRLENNRSRALKKSACFYRDIIKNRRFETEEFRYK
EEEEEECCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
ARQTWKIPDDFILGAAASAWQTEGWDGKRPTQDSYLDMWYKNDPDVWHDGYGPAVATDF
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHH
YNRYKEDIQYMKDIGLTHYRTSINWSRFLLDYETAEVDEVYAGYIDDVIDELKRCGVEPM
HHHHHHHHHHHHHCCCEEEECCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LCLEHYEIPTVLLEQYGGWGSKHVVGLFAAYAEKAFERYGDRVKYWFTFNEPIVPQTRIY
EHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHH
LDALRYPYEQNTKKWMQWNYHKALATAKAVAIFRKRKPSFREGAKIGVILNPEVAYARSS
HHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCC
APHDQKAAQVYDLFFNRVFLDPSIKGVYPDELMELMAKHDILFDHDEEELSVIKRNTVDF
CCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHCCEEE
VGVNLYYPKRVQAPSRQWNESTPFHPAYYYEPFELPGRKMNPFRGWEICPRIVYDMAMRL
EEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHE
KYEYGNIEWLIAENGMGVEHEERFKNEEGVIQDDYRIDFISAHLREAMKGIADGANCKGY
EEECCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEE
MLWAFTDNVSPMNAFKNRYGLVEIRLENNRSRALKKSACFYRDIIKNRRFETEEFRYK
EEEEEECCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]