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The map label for this gene is ansA [H]

Identifier: 52787817

GI number: 52787817

Start: 3967880

End: 3968869

Strand: Reverse

Name: ansA [H]

Synonym: BLi04140

Alternate gene names: 52787817

Gene position: 3968869-3967880 (Counterclockwise)

Preceding gene: 52787819

Following gene: 52787816

Centisome position: 93.99

GC content: 45.45

Gene sequence:

>990_bases
ATGAAAAAGTTACTGCTGTTGACCACCGGCGGGACCATCGCTTCAGTAGAAGGCGAAAACGGACTGGCTCCGGGGGTTAA
AGCGGAAGAGCTTCTCAGCTATTTATCTGATGATCATTCAAATTATACGATAGATTGCCAATCTTTAATGGATATAGACA
GCACCAACATGCAGCCTGAACACTGGGTGATGATGGCTGAGGCTGTATATGAAAATTACAGCCGCTACGACGGCTTTGTG
ATCACGCACGGAACCGATACGATGGCCTACACCTCAGCGGCGCTTTCATACATGCTGCAGAATATTGATAAGCCCGTCGC
GATTACGGGTTCCCAAGTTCCGATTACGTTTAAGAAAACGGATGCGAAAAAAAATATAAAAGATGCGGTCCGCTTTGCGT
GCGACGGCATCGGCGGAGTATATGTCGTCTTTGACGGCCGCGTCATTTTAGGCACGAGAGCGATCAAATTAAGAACAAAA
AGCTACGATGCATTTGAAAGCATCAATTATCCTTACATCGCTTTCATTCACGATAAGGAAATCGAGTACAACAAACGGGT
TCCCGAAGTAAAGCCAGGCGCTTTGAAACTCGATACCTCTTTAAATACCGACGTTTGTCTGGTGAAGCTTCATCCGGGTT
TAAAACCAGAGTTTTTCGATTGTCTGAAAGGTTCATATAAAGGCATTGTGATTGAGAGCTACGGAAGCGGCGGCATTCCT
TTTGAGAAAAGAAACATTTTAGAAAAAGTGAACGAGCTGATCGATTCCGGTATGGTCGTAGCGATCACGACGCAATGCCT
TGAAGAAGGAGAGGATATGAGCATTTACGAGGTCGGCCGCAAGGTAAACCAGGATGCCATTATCCGCTCGAGAAATATGA
ACACTGAAGCGATCGTTCCGAAGCTGATGTGGGCTTTAGGGAAAAACGGACAACAAGCTGAAGTCAAAAAGATCATGGAA
ACGCCGATTGCAGACGATATCATCATATAA

Upstream 100 bases:

>100_bases
TACGCATAAAGCGTATACATTCCTGATGTATCAAGCCTTCTTTGCTAAAATAAATCATGATTTATACTGTATAAAACTAC
TTCTTAAAGAGGTGTAGCAC

Downstream 100 bases:

>100_bases
AAAGAAAGCAGGGTTAAAAAATGAAAGCTGAAATGACATACCGGACGGAAAAGGATTTTCTCGGAGAGAAGAAGATTCCG
GCCGATGTCTACTACGGGAT

Product: AnsA

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKLLLLTTGGTIASVEGENGLAPGVKAEELLSYLSDDHSNYTIDCQSLMDIDSTNMQPEHWVMMAEAVYENYSRYDGFV
ITHGTDTMAYTSAALSYMLQNIDKPVAITGSQVPITFKKTDAKKNIKDAVRFACDGIGGVYVVFDGRVILGTRAIKLRTK
SYDAFESINYPYIAFIHDKEIEYNKRVPEVKPGALKLDTSLNTDVCLVKLHPGLKPEFFDCLKGSYKGIVIESYGSGGIP
FEKRNILEKVNELIDSGMVVAITTQCLEEGEDMSIYEVGRKVNQDAIIRSRNMNTEAIVPKLMWALGKNGQQAEVKKIME
TPIADDIII

Sequences:

>Translated_329_residues
MKKLLLLTTGGTIASVEGENGLAPGVKAEELLSYLSDDHSNYTIDCQSLMDIDSTNMQPEHWVMMAEAVYENYSRYDGFV
ITHGTDTMAYTSAALSYMLQNIDKPVAITGSQVPITFKKTDAKKNIKDAVRFACDGIGGVYVVFDGRVILGTRAIKLRTK
SYDAFESINYPYIAFIHDKEIEYNKRVPEVKPGALKLDTSLNTDVCLVKLHPGLKPEFFDCLKGSYKGIVIESYGSGGIP
FEKRNILEKVNELIDSGMVVAITTQCLEEGEDMSIYEVGRKVNQDAIIRSRNMNTEAIVPKLMWALGKNGQQAEVKKIME
TPIADDIII
>Mature_329_residues
MKKLLLLTTGGTIASVEGENGLAPGVKAEELLSYLSDDHSNYTIDCQSLMDIDSTNMQPEHWVMMAEAVYENYSRYDGFV
ITHGTDTMAYTSAALSYMLQNIDKPVAITGSQVPITFKKTDAKKNIKDAVRFACDGIGGVYVVFDGRVILGTRAIKLRTK
SYDAFESINYPYIAFIHDKEIEYNKRVPEVKPGALKLDTSLNTDVCLVKLHPGLKPEFFDCLKGSYKGIVIESYGSGGIP
FEKRNILEKVNELIDSGMVVAITTQCLEEGEDMSIYEVGRKVNQDAIIRSRNMNTEAIVPKLMWALGKNGQQAEVKKIME
TPIADDIII

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=355, Percent_Identity=30.4225352112676, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788065, Length=327, Percent_Identity=32.1100917431193, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1789327, Length=148, Percent_Identity=34.4594594594595, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=348, Percent_Identity=29.3103448275862, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17558280, Length=348, Percent_Identity=29.3103448275862, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI193207374, Length=348, Percent_Identity=29.3103448275862, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17558278, Length=348, Percent_Identity=29.3103448275862, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320527, Length=266, Percent_Identity=27.0676691729323, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=255, Percent_Identity=26.2745098039216, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323187, Length=255, Percent_Identity=26.2745098039216, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323186, Length=255, Percent_Identity=26.2745098039216, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323184, Length=255, Percent_Identity=26.2745098039216, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24645530, Length=340, Percent_Identity=30.5882352941176, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24639609, Length=271, Percent_Identity=32.4723247232472, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36506; Mature: 36506

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLLTTGGTIASVEGENGLAPGVKAEELLSYLSDDHSNYTIDCQSLMDIDSTNMQPE
CCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCH
HWVMMAEAVYENYSRYDGFVITHGTDTMAYTSAALSYMLQNIDKPVAITGSQVPITFKKT
HHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECC
DAKKNIKDAVRFACDGIGGVYVVFDGRVILGTRAIKLRTKSYDAFESINYPYIAFIHDKE
CCHHHHHHHHHHHHCCCCCEEEEECCEEEEEEEEEEEEECCCHHHHHCCCCEEEEEECCC
IEYNKRVPEVKPGALKLDTSLNTDVCLVKLHPGLKPEFFDCLKGSYKGIVIESYGSGGIP
CCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCC
FEKRNILEKVNELIDSGMVVAITTQCLEEGEDMSIYEVGRKVNQDAIIRSRNMNTEAIVP
HHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCHHEECCCCCHHHHHH
KLMWALGKNGQQAEVKKIMETPIADDIII
HHHHHHCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKLLLLTTGGTIASVEGENGLAPGVKAEELLSYLSDDHSNYTIDCQSLMDIDSTNMQPE
CCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCH
HWVMMAEAVYENYSRYDGFVITHGTDTMAYTSAALSYMLQNIDKPVAITGSQVPITFKKT
HHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECC
DAKKNIKDAVRFACDGIGGVYVVFDGRVILGTRAIKLRTKSYDAFESINYPYIAFIHDKE
CCHHHHHHHHHHHHCCCCCEEEEECCEEEEEEEEEEEEECCCHHHHHCCCCEEEEEECCC
IEYNKRVPEVKPGALKLDTSLNTDVCLVKLHPGLKPEFFDCLKGSYKGIVIESYGSGGIP
CCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCC
FEKRNILEKVNELIDSGMVVAITTQCLEEGEDMSIYEVGRKVNQDAIIRSRNMNTEAIVP
HHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCHHEECCCCCHHHHHH
KLMWALGKNGQQAEVKKIMETPIADDIII
HHHHHHCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]