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The map label for this gene is srlA [H]

Identifier: 52787545

GI number: 52787545

Start: 3693051

End: 3693599

Strand: Reverse

Name: srlA [H]

Synonym: BLi03866

Alternate gene names: 52787545

Gene position: 3693599-3693051 (Counterclockwise)

Preceding gene: 52787546

Following gene: 52787544

Centisome position: 87.47

GC content: 47.54

Gene sequence:

>549_bases
ATGAAAAACGTAACATGGCTTGCGGAAAAGTTTATGGACTTATTCAGGCATGGCGGAGAAGTGTTTGTCGGTATGCTTAC
AGACATCGTTCCTTTATTGATTATGCTGCTTGTCGCCATGAATGCTCTGATACGCCTGATAGGAGAAAAGCGCGTAGAAG
CGCTTGCGAGGAAGTCGGGAAAAAATCCATTGACCAGATACATGGTTTTGCCTGTCATCGGTACGTTCATGCTCGCAAAT
CCAATGACGCTGTCGCTTGGCAGGTTCTTGCCTGAAAAATACAAACCGAGCTATTATGCGGCTGCATCCTATTCGTGCCA
CACGCATAACGGACTTTTCCCACACGTGAATCCGGGCGAGCTGTTTGTTTTCCTTGGGATTGCCGCAGGGATTACAACGC
TCGGGCATGAAACGACGGAGCTTGCTCTCCGCTATCTTTTAGTCGGGATTGTGACAAACTTTCTCAGGGGCTGGGTAACG
GATATGACAACGGCTTATGTCGCGAAACAGCAGGGCATTGCCTTGAAAGATTCGATTGATCTTAAATAA

Upstream 100 bases:

>100_bases
TGAGAACATTCGACATCATCACGAAAGGGGGTGATGTTCCGCAGAAGAAAACACGGCTTCAAAAACTGATGAGCTTTCAA
ATCAAAAAAAGAGGTGAAAT

Downstream 100 bases:

>100_bases
GAAAGGCGGTTTAGGAGATGAGTTCCTATCGTTCGGTTGAAGTTTCAAAAGGGCCTGGAGGGTTTGGCGGACCTCTGGTG
ATCACGCCTGATGAAGAGAG

Product: hypothetical protein

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gut; PTS system glucitol/sorbitol-specific EIIC component [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKNVTWLAEKFMDLFRHGGEVFVGMLTDIVPLLIMLLVAMNALIRLIGEKRVEALARKSGKNPLTRYMVLPVIGTFMLAN
PMTLSLGRFLPEKYKPSYYAAASYSCHTHNGLFPHVNPGELFVFLGIAAGITTLGHETTELALRYLLVGIVTNFLRGWVT
DMTTAYVAKQQGIALKDSIDLK

Sequences:

>Translated_182_residues
MKNVTWLAEKFMDLFRHGGEVFVGMLTDIVPLLIMLLVAMNALIRLIGEKRVEALARKSGKNPLTRYMVLPVIGTFMLAN
PMTLSLGRFLPEKYKPSYYAAASYSCHTHNGLFPHVNPGELFVFLGIAAGITTLGHETTELALRYLLVGIVTNFLRGWVT
DMTTAYVAKQQGIALKDSIDLK
>Mature_182_residues
MKNVTWLAEKFMDLFRHGGEVFVGMLTDIVPLLIMLLVAMNALIRLIGEKRVEALARKSGKNPLTRYMVLPVIGTFMLAN
PMTLSLGRFLPEKYKPSYYAAASYSCHTHNGLFPHVNPGELFVFLGIAAGITTLGHETTELALRYLLVGIVTNFLRGWVT
DMTTAYVAKQQGIALKDSIDLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3730

COG function: function code G; Phosphotransferase system sorbitol-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994904, Length=181, Percent_Identity=63.5359116022099, Blast_Score=245, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004699 [H]

Pfam domain/function: PF03608 EII-GUT [H]

EC number: NA

Molecular weight: Translated: 20233; Mature: 20233

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS51107 PTS_EIIC_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVTWLAEKFMDLFRHGGEVFVGMLTDIVPLLIMLLVAMNALIRLIGEKRVEALARKSG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNPLTRYMVLPVIGTFMLANPMTLSLGRFLPEKYKPSYYAAASYSCHTHNGLFPHVNPGE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCCCCCCCH
LFVFLGIAAGITTLGHETTELALRYLLVGIVTNFLRGWVTDMTTAYVAKQQGIALKDSID
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
LK
CC
>Mature Secondary Structure
MKNVTWLAEKFMDLFRHGGEVFVGMLTDIVPLLIMLLVAMNALIRLIGEKRVEALARKSG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNPLTRYMVLPVIGTFMLANPMTLSLGRFLPEKYKPSYYAAASYSCHTHNGLFPHVNPGE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCCCCCCCH
LFVFLGIAAGITTLGHETTELALRYLLVGIVTNFLRGWVTDMTTAYVAKQQGIALKDSID
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; sorbitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9435786 [H]