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The map label for this gene is ywsC [H]

Identifier: 52787519

GI number: 52787519

Start: 3666404

End: 3667633

Strand: Reverse

Name: ywsC [H]

Synonym: BLi03839

Alternate gene names: 52787519

Gene position: 3667633-3666404 (Counterclockwise)

Preceding gene: 52787526

Following gene: 52787518

Centisome position: 86.86

GC content: 45.2

Gene sequence:

>1230_bases
ATGAATGAATTTACATATCAGATTCCAAGAAGGAGATGTAGACAAACAATGTGGGTAATGCTATTAGCCTGTGTGATCGT
TGTTGGGATCGGCATTTATGAAAAAAGGCGCCACCAGCAAAATATCGATGCGCTGCCTGTCCGAGTGAACATCAACGGTA
TACGCGGAAAGTCCACGGTGACAAGATTAACAACAGGGATATTAATCGAAGCAGGCTACAAAACAGTAGGAAAAACAACC
GGGACAGACGCAAGGATGATTTATTGGGACACACCGGAAGAGAAGCCGATCAAAAGAAAGCCGCAAGGGCCGAATATCGG
AGAGCAGAAGGAGGTTATGAAAGAAACGGTGGAAAGAGGGGCCAATGCGATTGTCAGTGAGTGCATGGCCGTTAATCCTG
ATTACCAAATCATCTTTCAGGAAGAATTGCTTCAGGCTAATATCGGCGTGATCGTGAACGTGCTGGAGGATCACATGGAT
GTGATGGGACCGACTTTGGATGAAATCGCAGAAGCATTCACAGCAACCATTCCTTATAATGGACATTTGGTTATTACTGA
TAGTGAGTATACCGATTTCTTTAAGCAAATTGCAAAAGAAAGGAACACAAAAGTCATCGTCGCAGACAATTCTAAAATAA
CAGATGAATACCTCAGACAGTTTGAGTACATGGTATTCCCTGATAATGCGTCTCTTGCGCTCGGTGTAGCTCAAGCGTTG
GGCATTGACGAAGAAACCGCCTTTAAAGGCATGCTGAATGCGCCGCCTGATCCGGGAGCCATGAGAATTCTGCCGCTGAT
GAACGCCAAGAATCCCGGACATTTCGTCAACGGTTTTGCGGCCAATGACGCAGCTTCCACTTTAAACATTTGGAAGCGTG
TAAAAGAAATAGGCTATCCTACGGATCAGCCGATCGTCATTATGAACTGCCGCGCCGACAGGGTAGACAGAACACAGCAG
TTTGCGGAAGATGTCCTTCCTTATATTGAAGCAAGTGAACTTGTGCTGATTGGAGAAACAACAGAGCCGATCGTCAAAGC
ATATGAAGCAGGCAAAATTCCTGCGGACAAGCTGTTTGATTTTGAGCACAAATCAACGGAAGAAATCATGTTCATGCTGA
AAAACAAGCTTGAGGGCCGCGTTATTTACGGAGTCGGAAATATCCACGGAGCAGCGGAGCCTCTCATTGAAAAAATACAA
GATTACAAGATTAAGCAGCTCGTTAGCTAG

Upstream 100 bases:

>100_bases
AGGCATTAATGACTCGGGCGAGGTGATCTTTGTCACGGAAAATTTCGTCGTCTTCTGTTACATAATGCCGATTGTGATTT
CATAGTGAACCTATATACTG

Downstream 100 bases:

>100_bases
GAGGAAGAAAACACAATGTTTGGATCAGATTTATATATCGCCCTCATTTTAGGAGTCTTACTCAGTTTGATTTTTGCAGA
GAAAACGGGAATTGTACCAG

Product: YwsC

Products: NA

Alternate protein names: Gamma-polyglutamic acid synthase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MNEFTYQIPRRRCRQTMWVMLLACVIVVGIGIYEKRRHQQNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTVGKTT
GTDARMIYWDTPEEKPIKRKPQGPNIGEQKEVMKETVERGANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMD
VMGPTLDEIAEAFTATIPYNGHLVITDSEYTDFFKQIAKERNTKVIVADNSKITDEYLRQFEYMVFPDNASLALGVAQAL
GIDEETAFKGMLNAPPDPGAMRILPLMNAKNPGHFVNGFAANDAASTLNIWKRVKEIGYPTDQPIVIMNCRADRVDRTQQ
FAEDVLPYIEASELVLIGETTEPIVKAYEAGKIPADKLFDFEHKSTEEIMFMLKNKLEGRVIYGVGNIHGAAEPLIEKIQ
DYKIKQLVS

Sequences:

>Translated_409_residues
MNEFTYQIPRRRCRQTMWVMLLACVIVVGIGIYEKRRHQQNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTVGKTT
GTDARMIYWDTPEEKPIKRKPQGPNIGEQKEVMKETVERGANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMD
VMGPTLDEIAEAFTATIPYNGHLVITDSEYTDFFKQIAKERNTKVIVADNSKITDEYLRQFEYMVFPDNASLALGVAQAL
GIDEETAFKGMLNAPPDPGAMRILPLMNAKNPGHFVNGFAANDAASTLNIWKRVKEIGYPTDQPIVIMNCRADRVDRTQQ
FAEDVLPYIEASELVLIGETTEPIVKAYEAGKIPADKLFDFEHKSTEEIMFMLKNKLEGRVIYGVGNIHGAAEPLIEKIQ
DYKIKQLVS
>Mature_409_residues
MNEFTYQIPRRRCRQTMWVMLLACVIVVGIGIYEKRRHQQNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTVGKTT
GTDARMIYWDTPEEKPIKRKPQGPNIGEQKEVMKETVERGANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMD
VMGPTLDEIAEAFTATIPYNGHLVITDSEYTDFFKQIAKERNTKVIVADNSKITDEYLRQFEYMVFPDNASLALGVAQAL
GIDEETAFKGMLNAPPDPGAMRILPLMNAKNPGHFVNGFAANDAASTLNIWKRVKEIGYPTDQPIVIMNCRADRVDRTQQ
FAEDVLPYIEASELVLIGETTEPIVKAYEAGKIPADKLFDFEHKSTEEIMFMLKNKLEGRVIYGVGNIHGAAEPLIEKIQ
DYKIKQLVS

Specific function: Catalyzes the biosynthesis of PGA (gamma-polyglutamic acid) from L-glutamate. Both the 44-kDa and the 33-kDa forms are required for PGA synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008337
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: NA

Molecular weight: Translated: 45959; Mature: 45959

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFTYQIPRRRCRQTMWVMLLACVIVVGIGIYEKRRHQQNIDALPVRVNINGIRGKSTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCHHH
TRLTTGILIEAGYKTVGKTTGTDARMIYWDTPEEKPIKRKPQGPNIGEQKEVMKETVERG
HHHHHHHEEECCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMDVMGPTLDEIAEAFTATIPYN
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCC
GHLVITDSEYTDFFKQIAKERNTKVIVADNSKITDEYLRQFEYMVFPDNASLALGVAQAL
CEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCHHHHHHHHH
GIDEETAFKGMLNAPPDPGAMRILPLMNAKNPGHFVNGFAANDAASTLNIWKRVKEIGYP
CCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEECCEECCCHHHHHHHHHHHHHHCCC
TDQPIVIMNCRADRVDRTQQFAEDVLPYIEASELVLIGETTEPIVKAYEAGKIPADKLFD
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHCC
FEHKSTEEIMFMLKNKLEGRVIYGVGNIHGAAEPLIEKIQDYKIKQLVS
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEFTYQIPRRRCRQTMWVMLLACVIVVGIGIYEKRRHQQNIDALPVRVNINGIRGKSTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCHHH
TRLTTGILIEAGYKTVGKTTGTDARMIYWDTPEEKPIKRKPQGPNIGEQKEVMKETVERG
HHHHHHHEEECCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMDVMGPTLDEIAEAFTATIPYN
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCC
GHLVITDSEYTDFFKQIAKERNTKVIVADNSKITDEYLRQFEYMVFPDNASLALGVAQAL
CEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCHHHHHHHHH
GIDEETAFKGMLNAPPDPGAMRILPLMNAKNPGHFVNGFAANDAASTLNIWKRVKEIGYP
CCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEECCEECCCHHHHHHHHHHHHHHCCC
TDQPIVIMNCRADRVDRTQQFAEDVLPYIEASELVLIGETTEPIVKAYEAGKIPADKLFD
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHCC
FEHKSTEEIMFMLKNKLEGRVIYGVGNIHGAAEPLIEKIQDYKIKQLVS
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 10486244; 11751809; 9384377 [H]