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The map label for this gene is ywtD [H]

Identifier: 52787515

GI number: 52787515

Start: 3662885

End: 3664129

Strand: Reverse

Name: ywtD [H]

Synonym: BLi03835

Alternate gene names: 52787515

Gene position: 3664129-3662885 (Counterclockwise)

Preceding gene: 52787516

Following gene: 52787512

Centisome position: 86.77

GC content: 47.71

Gene sequence:

>1245_bases
TTGATAAAAAAAGCGGCAAACAAAAAGTTGGTTTTGTTTTGTGGAATTGCGGTGCTTTGGATGTCTTTATTTTTAACGAA
TCATAATGATGTACGCGCCGATACGATCGGCGAGAAAATAGCGGAAACTGCCAGACAGCTTGAGGGTGCGAAATACAGCT
ACGGCGGAGAGAAGCCGAAAACGGGGTTTGACTCGTCAGGCTTTGTGCAATATGTGTTTCAATCGCTCGATATTACGCTT
CCGAGAACGGTAAAGGAACAATCGACTCTTGGGAGCAGTGTCGGCCGTCAGCAGCTCGAAAAGGGGGACCTTGTCTTTTT
CAAGAATGCCGAGCTGGAATCGGACGGACCGACCCATGTCGCCATCTATTTGGGAAATGATCAAATCATCCACAGCACAA
AATCAAACGGGGTTGTCGTGACAAAGCTTGAAGGCAGCTCTTACTGGAGCTCGGGGTATTTTAAAGCGAAAAGGATCACA
AAAGAGCCTGAGATTTCGATGGATCCTGTCGTTCAAAAAGCAAAAAGCTATGTCGGTGTTCCTTATGTATTTGGAGGCAA
CTCTCCGGATCTCGGATTTGACTGTTCGGGGTTGACCCAATACGTCTTCAGAGAGGTGCTCGGCGTTTATTTGCCAAGGT
CGGCTGAACAGCAATGGGCTGTCGGTCAAAAGGTGAAGCTTGAAGATATCCGGCCGGGTGATGTTTTGTTTTTCAGCAAT
ACGTACAAACCGGGAATATCCCATAACGGCATCTATGCCGGGGGCGGGCGGTTTATCCATGCGAGCCGTTCAAATAAAGT
GACGATATCCTACTTGTCGGCTTCCTATTGGCAGAAGAAGTTCACAGGAGTCAGACGTTTTGACAACATGTCCCTGCCAA
AAAATCCGATTGTATCCGAAGCCATCAGGCATATCGGCGAAGTCGGTTATCAAAAAGGCGGCACATCGCCTAAAGAAGGC
TTTGATACGGCTGGGTTTATCCAATATGTCTACAAAACGGCGGCAGGAGTGGAGCTTCCGAGGTATGCTGACAAACAATA
CAGCACGGGTAAGAAAATTACCAAACAGGAGCTTGAGCCTGGAGACATCGTCTTCTTTAAAGGAACCACTGTTATGAATC
CCGCCATCTATATCGGAAACGGCCAGGTCGTTCTTGTCACCTTGTCTGCCGGTGTAACGACAGCAGATATGGAGACGAGC
GCCTATTGGAAAGATAAATACGCCGGAAGCGTCAGAATTGAGTAG

Upstream 100 bases:

>100_bases
GTTTCTTCTCGCTATGACGCTGCTGTACATGCAAAAAAAGTCCTTTAAATATCCCAGTTGAATGACGATGAAAGAGGAAA
GAAGAGGAGGAACAGATCAA

Downstream 100 bases:

>100_bases
CAGAAACAGCAAAAAAACCGCTCGAAGATGGACTCGAGCGGTTTTCTTATTTATTGATATTCATTTTCCAGCAGTTTGTT
AATGAAATAAGCGACGCCGT

Product: YwtD

Products: NA

Alternate protein names: Poly-gamma-glutamate depolymerase; PGA depolymerase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MIKKAANKKLVLFCGIAVLWMSLFLTNHNDVRADTIGEKIAETARQLEGAKYSYGGEKPKTGFDSSGFVQYVFQSLDITL
PRTVKEQSTLGSSVGRQQLEKGDLVFFKNAELESDGPTHVAIYLGNDQIIHSTKSNGVVVTKLEGSSYWSSGYFKAKRIT
KEPEISMDPVVQKAKSYVGVPYVFGGNSPDLGFDCSGLTQYVFREVLGVYLPRSAEQQWAVGQKVKLEDIRPGDVLFFSN
TYKPGISHNGIYAGGGRFIHASRSNKVTISYLSASYWQKKFTGVRRFDNMSLPKNPIVSEAIRHIGEVGYQKGGTSPKEG
FDTAGFIQYVYKTAAGVELPRYADKQYSTGKKITKQELEPGDIVFFKGTTVMNPAIYIGNGQVVLVTLSAGVTTADMETS
AYWKDKYAGSVRIE

Sequences:

>Translated_414_residues
MIKKAANKKLVLFCGIAVLWMSLFLTNHNDVRADTIGEKIAETARQLEGAKYSYGGEKPKTGFDSSGFVQYVFQSLDITL
PRTVKEQSTLGSSVGRQQLEKGDLVFFKNAELESDGPTHVAIYLGNDQIIHSTKSNGVVVTKLEGSSYWSSGYFKAKRIT
KEPEISMDPVVQKAKSYVGVPYVFGGNSPDLGFDCSGLTQYVFREVLGVYLPRSAEQQWAVGQKVKLEDIRPGDVLFFSN
TYKPGISHNGIYAGGGRFIHASRSNKVTISYLSASYWQKKFTGVRRFDNMSLPKNPIVSEAIRHIGEVGYQKGGTSPKEG
FDTAGFIQYVYKTAAGVELPRYADKQYSTGKKITKQELEPGDIVFFKGTTVMNPAIYIGNGQVVLVTLSAGVTTADMETS
AYWKDKYAGSVRIE
>Mature_414_residues
MIKKAANKKLVLFCGIAVLWMSLFLTNHNDVRADTIGEKIAETARQLEGAKYSYGGEKPKTGFDSSGFVQYVFQSLDITL
PRTVKEQSTLGSSVGRQQLEKGDLVFFKNAELESDGPTHVAIYLGNDQIIHSTKSNGVVVTKLEGSSYWSSGYFKAKRIT
KEPEISMDPVVQKAKSYVGVPYVFGGNSPDLGFDCSGLTQYVFREVLGVYLPRSAEQQWAVGQKVKLEDIRPGDVLFFSN
TYKPGISHNGIYAGGGRFIHASRSNKVTISYLSASYWQKKFTGVRRFDNMSLPKNPIVSEAIRHIGEVGYQKGGTSPKEG
FDTAGFIQYVYKTAAGVELPRYADKQYSTGKKITKQELEPGDIVFFKGTTVMNPAIYIGNGQVVLVTLSAGVTTADMETS
AYWKDKYAGSVRIE

Specific function: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA) [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133 [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=132, Percent_Identity=32.5757575757576, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786421, Length=120, Percent_Identity=38.3333333333333, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788501, Length=133, Percent_Identity=37.593984962406, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788001, Length=114, Percent_Identity=37.719298245614, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 45615; Mature: 45615

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKAANKKLVLFCGIAVLWMSLFLTNHNDVRADTIGEKIAETARQLEGAKYSYGGEKPK
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCC
TGFDSSGFVQYVFQSLDITLPRTVKEQSTLGSSVGRQQLEKGDLVFFKNAELESDGPTHV
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEE
AIYLGNDQIIHSTKSNGVVVTKLEGSSYWSSGYFKAKRITKEPEISMDPVVQKAKSYVGV
EEEECCCEEEEECCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCC
PYVFGGNSPDLGFDCSGLTQYVFREVLGVYLPRSAEQQWAVGQKVKLEDIRPGDVLFFSN
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCEEEEEC
TYKPGISHNGIYAGGGRFIHASRSNKVTISYLSASYWQKKFTGVRRFDNMSLPKNPIVSE
CCCCCCCCCCEEECCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHH
AIRHIGEVGYQKGGTSPKEGFDTAGFIQYVYKTAAGVELPRYADKQYSTGKKITKQELEP
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCC
GDIVFFKGTTVMNPAIYIGNGQVVLVTLSAGVTTADMETSAYWKDKYAGSVRIE
CCEEEEECCEEECCEEEECCCEEEEEEEECCCEEECCCCCHHECCCCCCEEEEC
>Mature Secondary Structure
MIKKAANKKLVLFCGIAVLWMSLFLTNHNDVRADTIGEKIAETARQLEGAKYSYGGEKPK
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCC
TGFDSSGFVQYVFQSLDITLPRTVKEQSTLGSSVGRQQLEKGDLVFFKNAELESDGPTHV
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEE
AIYLGNDQIIHSTKSNGVVVTKLEGSSYWSSGYFKAKRITKEPEISMDPVVQKAKSYVGV
EEEECCCEEEEECCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCC
PYVFGGNSPDLGFDCSGLTQYVFREVLGVYLPRSAEQQWAVGQKVKLEDIRPGDVLFFSN
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCEEEEEC
TYKPGISHNGIYAGGGRFIHASRSNKVTISYLSASYWQKKFTGVRRFDNMSLPKNPIVSE
CCCCCCCCCCEEECCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHH
AIRHIGEVGYQKGGTSPKEGFDTAGFIQYVYKTAAGVELPRYADKQYSTGKKITKQELEP
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCC
GDIVFFKGTTVMNPAIYIGNGQVVLVTLSAGVTTADMETSAYWKDKYAGSVRIE
CCEEEEECCEEECCEEEECCCEEEEEEEECCCEEECCCCCHHECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12073126; 12644511; 9353933; 9384377; 10658653 [H]