Click here to switch to the map view.

The map label for this gene is ycbU [H]

Identifier: 52787221

GI number: 52787221

Start: 3372664

End: 3373788

Strand: Direct

Name: ycbU [H]

Synonym: BLi03531

Alternate gene names: 52787221

Gene position: 3372664-3373788 (Clockwise)

Preceding gene: 52787218

Following gene: 52787222

Centisome position: 79.87

GC content: 50.76

Gene sequence:

>1125_bases
ATGAAGCATCCATTCGACAATTTCCGTTCTTTATTCCCGAAATTGTCTGATCATGTTCATCTTGCCGCATGTTCCGGAAG
CGCATTGGCTCTGCCTGTTGCCAAAGCGGTCGATGAGTATCAGAACGGCCTTTTAACAGCCGGTGCAGACTGGAAAGCAC
ATCTCGACAAGATGGATGAGACCAAGGAAAAATTCGCCCGGCTGATCGGTGCTGAAAAAGAAGAGATAGCCGTTTTGCCG
TCAGTATCTGACGCAGTGGCCGCTGTGGCCGCCGCAATGCCGCAGCCACCCCGTCGAAGGCAGATCGCCTTCACGGATAT
CGATTTTCCTGCTGTCGGCCAAATTTGGCTTGCGCAAGACCGCTTCAAAGATCACCTCATCATCATTCAAAGCGAAGATG
GGATGATTGACTTGGAACAATACGAAAAAGACGTTACCGATCAGACGCTCTTGACGTGTGTGCCCCATGCCGGCTATCGG
AACGGGTTTAAGCAAGACATAAAGCAGATTGCCGAGATTGTTCATCAGAAAGGGTCGCTTTTATTCGTCGACGCCTATCA
GACTGCCGGCCACATTCCGATTCATGTCAAAGACATGGATATCGACATCCTTGCGGCGGGGACGCGCAAATACATGCTCG
GCATTCCCGGAACAGCTTTTTTATATATGAAACGCGAACTTTGCGAACAATTTGAGCCAAAAACGGCCGGCTGGTTCGGA
AGGAGCGCGCCATTTGCCTTAGATTCAGGCTTTGCGGAAGGTGCGAAACGCTTCGAGTCGGGGACACCGTCATTTATCAG
CATATATGCGGCAGACGCGGCATTGAAGCTGCTGCTTGAAGCGGAGCCCGACAGGATCGAGCCCTATCTGCGGCAGCTTG
CGGCATTCTCTTTGCAATACGGTGCGGAAAAAGGCCTGAAGATCACAGGACCCAACACCGTACATAACAGAACAAGCCTG
ATCTCGGTGTATGCCGAAGAAGCCGCCCGTGTTGAATCCCTCTTGCGTGAAAAGCATATCATCGTCTCAAAGCGAAACAA
CGTCATAAGAATAGCCCCGCATTTTTACAATACAAAGGAGGAAATCGGATTTGTGATGGATGAGATCGCGGCAATCGTAG
GCTAA

Upstream 100 bases:

>100_bases
TTCGCTTTTTCGCTTAAATAGGCTTCTGACCGCTTGATCGTAACCGTGAAAAAGAGGATTTTTTCAAGCCCCGCTCGAAG
CTTTAATAAGGAGGGATATC

Downstream 100 bases:

>100_bases
GCCCCCGGCTTTAAAATGGACAGAATGCGATTTTTTCCTTAAACTATAAAAAACAATTTTCATCAAGGAATGAGACAAAA
TGGAGCTTTATATCGAAGGT

Product: YcbU

Products: hydrogen selenide; @ALAN01.txt*l-alanine!; acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKHPFDNFRSLFPKLSDHVHLAACSGSALALPVAKAVDEYQNGLLTAGADWKAHLDKMDETKEKFARLIGAEKEEIAVLP
SVSDAVAAVAAAMPQPPRRRQIAFTDIDFPAVGQIWLAQDRFKDHLIIIQSEDGMIDLEQYEKDVTDQTLLTCVPHAGYR
NGFKQDIKQIAEIVHQKGSLLFVDAYQTAGHIPIHVKDMDIDILAAGTRKYMLGIPGTAFLYMKRELCEQFEPKTAGWFG
RSAPFALDSGFAEGAKRFESGTPSFISIYAADAALKLLLEAEPDRIEPYLRQLAAFSLQYGAEKGLKITGPNTVHNRTSL
ISVYAEEAARVESLLREKHIIVSKRNNVIRIAPHFYNTKEEIGFVMDEIAAIVG

Sequences:

>Translated_374_residues
MKHPFDNFRSLFPKLSDHVHLAACSGSALALPVAKAVDEYQNGLLTAGADWKAHLDKMDETKEKFARLIGAEKEEIAVLP
SVSDAVAAVAAAMPQPPRRRQIAFTDIDFPAVGQIWLAQDRFKDHLIIIQSEDGMIDLEQYEKDVTDQTLLTCVPHAGYR
NGFKQDIKQIAEIVHQKGSLLFVDAYQTAGHIPIHVKDMDIDILAAGTRKYMLGIPGTAFLYMKRELCEQFEPKTAGWFG
RSAPFALDSGFAEGAKRFESGTPSFISIYAADAALKLLLEAEPDRIEPYLRQLAAFSLQYGAEKGLKITGPNTVHNRTSL
ISVYAEEAARVESLLREKHIIVSKRNNVIRIAPHFYNTKEEIGFVMDEIAAIVG
>Mature_374_residues
MKHPFDNFRSLFPKLSDHVHLAACSGSALALPVAKAVDEYQNGLLTAGADWKAHLDKMDETKEKFARLIGAEKEEIAVLP
SVSDAVAAVAAAMPQPPRRRQIAFTDIDFPAVGQIWLAQDRFKDHLIIIQSEDGMIDLEQYEKDVTDQTLLTCVPHAGYR
NGFKQDIKQIAEIVHQKGSLLFVDAYQTAGHIPIHVKDMDIDILAAGTRKYMLGIPGTAFLYMKRELCEQFEPKTAGWFG
RSAPFALDSGFAEGAKRFESGTPSFISIYAADAALKLLLEAEPDRIEPYLRQLAAFSLQYGAEKGLKITGPNTVHNRTSL
ISVYAEEAARVESLLREKHIIVSKRNNVIRIAPHFYNTKEEIGFVMDEIAAIVG

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=405, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994898, Length=191, Percent_Identity=27.2251308900524, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17550466, Length=410, Percent_Identity=24.8780487804878, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129463, Length=184, Percent_Identity=25.5434782608696, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.8.1.7 [C]

Molecular weight: Translated: 41498; Mature: 41498

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHPFDNFRSLFPKLSDHVHLAACSGSALALPVAKAVDEYQNGLLTAGADWKAHLDKMDE
CCCCHHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHCCEEECCCCHHHHHHHHHH
TKEKFARLIGAEKEEIAVLPSVSDAVAAVAAAMPQPPRRRQIAFTDIDFPAVGQIWLAQD
HHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEECC
RFKDHLIIIQSEDGMIDLEQYEKDVTDQTLLTCVPHAGYRNGFKQDIKQIAEIVHQKGSL
CCCCEEEEEECCCCEEEHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCE
LFVDAYQTAGHIPIHVKDMDIDILAAGTRKYMLGIPGTAFLYMKRELCEQFEPKTAGWFG
EEEEECCCCCCCEEEEEECCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
RSAPFALDSGFAEGAKRFESGTPSFISIYAADAALKLLLEAEPDRIEPYLRQLAAFSLQY
CCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GAEKGLKITGPNTVHNRTSLISVYAEEAARVESLLREKHIIVSKRNNVIRIAPHFYNTKE
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCHH
EIGFVMDEIAAIVG
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKHPFDNFRSLFPKLSDHVHLAACSGSALALPVAKAVDEYQNGLLTAGADWKAHLDKMDE
CCCCHHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHCCEEECCCCHHHHHHHHHH
TKEKFARLIGAEKEEIAVLPSVSDAVAAVAAAMPQPPRRRQIAFTDIDFPAVGQIWLAQD
HHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEECC
RFKDHLIIIQSEDGMIDLEQYEKDVTDQTLLTCVPHAGYRNGFKQDIKQIAEIVHQKGSL
CCCCEEEEEECCCCEEEHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCE
LFVDAYQTAGHIPIHVKDMDIDILAAGTRKYMLGIPGTAFLYMKRELCEQFEPKTAGWFG
EEEEECCCCCCCEEEEEECCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
RSAPFALDSGFAEGAKRFESGTPSFISIYAADAALKLLLEAEPDRIEPYLRQLAAFSLQY
CCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GAEKGLKITGPNTVHNRTSLISVYAEEAARVESLLREKHIIVSKRNNVIRIAPHFYNTKE
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCHH
EIGFVMDEIAAIVG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-selenocysteine; reduced acceptor [C]

Specific reaction: L-selenocysteine + reduced acceptor = hydrogen selenide + @ALAN01.txt*l-alanine! + acceptor [C]

General reaction: Elimination of H2S or RSH [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]