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The map label for this gene is yfiY [H]

Identifier: 52787166

GI number: 52787166

Start: 3315706

End: 3316683

Strand: Direct

Name: yfiY [H]

Synonym: BLi03475

Alternate gene names: 52787166

Gene position: 3315706-3316683 (Clockwise)

Preceding gene: 52787160

Following gene: 52787170

Centisome position: 78.52

GC content: 43.97

Gene sequence:

>978_bases
ATGAAGAGATGGTCAATTGTTGGATTTATCGCTTTGTTAGCGATTTCAATTCTTGCAGCCTGCGGCGGAGGCAAGGATGA
CTCTGCAAAGGGCGGAGAAAATAAAGATACGATCACCGTTAAACACGCAATGGGTACGGAAGACCAAGTGCCTGCAGACC
CAAAAAGAGTCGTCGTCCTGACAAATGAAGGAACCGAAGCGCTTTTGGAACTCGGAATCAAACCTGTGGGTGCAGTCAAA
TCCTGGACAGGCGATCCTTGGTATAAGCATATTCAAGCCGATATGAAAGGCGTTAAGCAAATCGGACTCGAAGGCGAACC
GAATATCGAAGCGATTGCCGAGCTGAAGCCTGATTTAATCATCGGGAATAAAATGAGACATGAAAAAATCTACGACAAGC
TTGAAAAAATTGCTCCGACTGTCTTCTCTGAAACGCTGCGCGGCGAATGGAAAGACAACTTCTCCCTCTACGCGAAAGCG
GTAAACAAAGAGGAAAAGGGCAAAGAAGTTTTAGAAGATTTCGATAAAAAAGTCAGCGAATTAAGCGACAAGCTCGGCGA
CCAGAAAAAGAAACGGGTGTCGGTAATCAGATTTATGGCCGGCCAATCAAGGATTTACTATAAAGATTCTTTCCCGGGAA
TTATCCTGGATCAGCTCGGATTCAAGTATCCGGAAAAACAGGAAAAACTGTTTGAGAAACAAGACGACAAATTTGTTTTT
ATGACGGAAAATAAAGAATCGATCCCTGATATGGACGGAGATGTCCTGTTCTATTTCACATATAAACCTGCCGAAAGCAA
AAAAGAAGCTGAAACATGGCAAAACGATTGGACAAGCGATCCGCTGTGGAAAAAATTGAACGCTGTAAAAACCGGAAACG
CCCATGAAGTCGACGATGCGATCTGGACGACGGCCGGCGGCGTAAAAGCAGCGTATCTGTTCCTTGATGACATCGAAAAA
TACTTTTTAAAATCTTAA

Upstream 100 bases:

>100_bases
TAACATTGATCTTGAAAATCATTTTCAATTAACAATTGACAAAATTCTGATCAAAAATTATGATCAGGATGAATGACTTA
CATACCTAAGGAGGACACAC

Downstream 100 bases:

>100_bases
GCAAAAAAGAGCATTTCCAGTTTGGAAATGCTCTTTTGTTTATTTTCCGTTATAGATTCTGGACGTGCCATCAGGCCATT
TCACCCGCAGCTGAAACCGG

Product: YfiY

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKRWSIVGFIALLAISILAACGGGKDDSAKGGENKDTITVKHAMGTEDQVPADPKRVVVLTNEGTEALLELGIKPVGAVK
SWTGDPWYKHIQADMKGVKQIGLEGEPNIEAIAELKPDLIIGNKMRHEKIYDKLEKIAPTVFSETLRGEWKDNFSLYAKA
VNKEEKGKEVLEDFDKKVSELSDKLGDQKKKRVSVIRFMAGQSRIYYKDSFPGIILDQLGFKYPEKQEKLFEKQDDKFVF
MTENKESIPDMDGDVLFYFTYKPAESKKEAETWQNDWTSDPLWKKLNAVKTGNAHEVDDAIWTTAGGVKAAYLFLDDIEK
YFLKS

Sequences:

>Translated_325_residues
MKRWSIVGFIALLAISILAACGGGKDDSAKGGENKDTITVKHAMGTEDQVPADPKRVVVLTNEGTEALLELGIKPVGAVK
SWTGDPWYKHIQADMKGVKQIGLEGEPNIEAIAELKPDLIIGNKMRHEKIYDKLEKIAPTVFSETLRGEWKDNFSLYAKA
VNKEEKGKEVLEDFDKKVSELSDKLGDQKKKRVSVIRFMAGQSRIYYKDSFPGIILDQLGFKYPEKQEKLFEKQDDKFVF
MTENKESIPDMDGDVLFYFTYKPAESKKEAETWQNDWTSDPLWKKLNAVKTGNAHEVDDAIWTTAGGVKAAYLFLDDIEK
YFLKS
>Mature_325_residues
MKRWSIVGFIALLAISILAACGGGKDDSAKGGENKDTITVKHAMGTEDQVPADPKRVVVLTNEGTEALLELGIKPVGAVK
SWTGDPWYKHIQADMKGVKQIGLEGEPNIEAIAELKPDLIIGNKMRHEKIYDKLEKIAPTVFSETLRGEWKDNFSLYAKA
VNKEEKGKEVLEDFDKKVSELSDKLGDQKKKRVSVIRFMAGQSRIYYKDSFPGIILDQLGFKYPEKQEKLFEKQDDKFVF
MTENKESIPDMDGDVLFYFTYKPAESKKEAETWQNDWTSDPLWKKLNAVKTGNAHEVDDAIWTTAGGVKAAYLFLDDIEK
YFLKS

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=275, Percent_Identity=24.7272727272727, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 36700; Mature: 36700

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWSIVGFIALLAISILAACGGGKDDSAKGGENKDTITVKHAMGTEDQVPADPKRVVVL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEE
TNEGTEALLELGIKPVGAVKSWTGDPWYKHIQADMKGVKQIGLEGEPNIEAIAELKPDLI
ECCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEE
IGNKMRHEKIYDKLEKIAPTVFSETLRGEWKDNFSLYAKAVNKEEKGKEVLEDFDKKVSE
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
LSDKLGDQKKKRVSVIRFMAGQSRIYYKDSFPGIILDQLGFKYPEKQEKLFEKQDDKFVF
HHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEE
MTENKESIPDMDGDVLFYFTYKPAESKKEAETWQNDWTSDPLWKKLNAVKTGNAHEVDDA
EECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHH
IWTTAGGVKAAYLFLDDIEKYFLKS
HHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRWSIVGFIALLAISILAACGGGKDDSAKGGENKDTITVKHAMGTEDQVPADPKRVVVL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEE
TNEGTEALLELGIKPVGAVKSWTGDPWYKHIQADMKGVKQIGLEGEPNIEAIAELKPDLI
ECCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEE
IGNKMRHEKIYDKLEKIAPTVFSETLRGEWKDNFSLYAKAVNKEEKGKEVLEDFDKKVSE
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
LSDKLGDQKKKRVSVIRFMAGQSRIYYKDSFPGIILDQLGFKYPEKQEKLFEKQDDKFVF
HHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEE
MTENKESIPDMDGDVLFYFTYKPAESKKEAETWQNDWTSDPLWKKLNAVKTGNAHEVDDA
EECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHH
IWTTAGGVKAAYLFLDDIEKYFLKS
HHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]