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The map label for this gene is pfkA [H]

Identifier: 52786796

GI number: 52786796

Start: 2954190

End: 2955149

Strand: Reverse

Name: pfkA [H]

Synonym: BLi03068

Alternate gene names: 52786796

Gene position: 2955149-2954190 (Counterclockwise)

Preceding gene: 52786797

Following gene: 52786795

Centisome position: 69.98

GC content: 47.4

Gene sequence:

>960_bases
ATGAAACGTATCGGAGTATTGACAAGCGGCGGAGATTCCCCGGGAATGAACGCGGCGGTCCGCGCGGTTGTCAGGAAAGC
GATCTACCATGACGTAGAAGTATACGGAATTTACAACGGGTATTCAGGTTTAATCAGCGGAAAAATAGAAAAGCTTGAAA
TCGGTTCTGTCGGAGATATCATTCACCGCGGAGGAACGAAGCTATATACAGCAAGGTGTCCTGAGTTTAAAACGGTAGAA
GGACGGGAAAAAGGAATAGCGAATTTAAAAAAATATGGTATAGAAGGATTAGTCGTCATTGGCGGCGACGGTTCTTATAT
GGGAGCCAAAAAGCTCACAGAACACGGTTTTCCTTGTGTGGGAGTGCCGGGAACGATCGACAATGACATCCCAGGCACTG
ATCTGACGATCGGATTCGATACGGCGCTAAATACCGTTATTGACGCCATCGATAAAATCAGGGATACAGCTACATCCCAT
GAACGTACATACGTGATTGAAGTAATGGGCCGCCATGCCGGGGACATTGCTTTATGGTCAGGTTTGGCCGGAGGCGCGGA
ATCCATTTTGATTCCTGAAGCAGACTACGATATGGAAGAAATTATTGCAAGGCTGAAAAGAGGGCATGAGCGCGGCAAGA
AACACAGCATCATCATTGTGGCGGAAGGCGTCGGCAGCGGCGTTGAATTCGGAAAAAGAATTGAAGAAGCCACAAATCTC
GAAACTAGAGTATCGGTGCTTGGACATATCCAGCGCGGCGGTTCCCCGACTGCAGCAGACCGCGTATTGGCAAGCCGTCT
AGGGGCATTTGCGGTTGAACTGCTTCTTGAAGGAAAAGGCGGACGTTGTGTAGGAATTCAAAATAACCAGCTTGTGCATC
ATGATATCATTGAAATACTCGAGCAAAAACATACAATTGATCAAAGCATGTACCGCTTGTCTCAAGAACTGTCAATCTAG

Upstream 100 bases:

>100_bases
GGTTTTATAGGGATTTTCGGAAAACTTTCATCCCTTTTTCATTGTTTTAGCGGCAATGAACGTGTTAAGGTAAATTATAT
ATGTTTTCGAGGTGAAAAAG

Downstream 100 bases:

>100_bases
TGTAAAACCCGAGGAGGAAGCAATATGAGAAAGACTAAAATAGTTTGTACAATTGGACCGGCCAGCGAAAGTGTTGAAAA
GCTCACCCAGCTGATGGAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLEIGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIANLKKYGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDLTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWSGLAGGAESILIPEADYDMEEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEATNL
ETRVSVLGHIQRGGSPTAADRVLASRLGAFAVELLLEGKGGRCVGIQNNQLVHHDIIEILEQKHTIDQSMYRLSQELSI

Sequences:

>Translated_319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLEIGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIANLKKYGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDLTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWSGLAGGAESILIPEADYDMEEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEATNL
ETRVSVLGHIQRGGSPTAADRVLASRLGAFAVELLLEGKGGRCVGIQNNQLVHHDIIEILEQKHTIDQSMYRLSQELSI
>Mature_319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLEIGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIANLKKYGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDLTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWSGLAGGAESILIPEADYDMEEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEATNL
ETRVSVLGHIQRGGSPTAADRVLASRLGAFAVELLLEGKGGRCVGIQNNQLVHHDIIEILEQKHTIDQSMYRLSQELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=347, Percent_Identity=43.2276657060519, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI48762920, Length=347, Percent_Identity=42.3631123919308, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI266453619, Length=349, Percent_Identity=42.9799426934097, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI266453768, Length=349, Percent_Identity=42.9799426934097, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI266453748, Length=349, Percent_Identity=42.9799426934097, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI4505749, Length=349, Percent_Identity=42.9799426934097, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1790350, Length=302, Percent_Identity=58.2781456953642, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25147584, Length=347, Percent_Identity=40.0576368876081, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=40.7766990291262, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=34.4827586206897, Blast_Score=165, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=38.953488372093, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=338, Percent_Identity=36.6863905325444, Blast_Score=192, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=41.3793103448276, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17647809, Length=348, Percent_Identity=41.3793103448276, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=40.6340057636888, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34285; Mature: 34285

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLEIGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCEEEEECCCHHHH
IHRGGTKLYTARCPEFKTVEGREKGIANLKKYGIEGLVVIGGDGSYMGAKKLTEHGFPCV
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCEE
GVPGTIDNDIPGTDLTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHC
GLAGGAESILIPEADYDMEEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEATNL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCH
ETRVSVLGHIQRGGSPTAADRVLASRLGAFAVELLLEGKGGRCVGIQNNQLVHHDIIEIL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHH
EQKHTIDQSMYRLSQELSI
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYSGLISGKIEKLEIGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCEEEEECCCHHHH
IHRGGTKLYTARCPEFKTVEGREKGIANLKKYGIEGLVVIGGDGSYMGAKKLTEHGFPCV
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCEE
GVPGTIDNDIPGTDLTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHC
GLAGGAESILIPEADYDMEEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEATNL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCH
ETRVSVLGHIQRGGSPTAADRVLASRLGAFAVELLLEGKGGRCVGIQNNQLVHHDIIEIL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHH
EQKHTIDQSMYRLSQELSI
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA