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The map label for this gene is pyk2

Identifier: 52786795

GI number: 52786795

Start: 2952408

End: 2954165

Strand: Reverse

Name: pyk2

Synonym: BLi03067

Alternate gene names: 52786795

Gene position: 2954165-2952408 (Counterclockwise)

Preceding gene: 52786796

Following gene: 52786794

Centisome position: 69.96

GC content: 50.4

Gene sequence:

>1758_bases
ATGAGAAAGACTAAAATAGTTTGTACAATTGGACCGGCCAGCGAAAGTGTTGAAAAGCTCACCCAGCTGATGGAAGCCGG
AATGAACGTGGCGCGCCTGAACTTTTCCCATGGAGATTTCGAAGAGCACGGCGCAAGAATTAAAAACATTCGTGAAGCAG
CCGGCAAGCTGGGAAAAGATATCGGCATCCTTCTTGACACGAAAGGGCCTGAAATCCGCACGCACACAATGGAAAACGGT
TCGATCGAACTGGCAGCGGGTTCTCAGCTGATCGTTTCAATGGACGAAGTCATCGGTACGCCTGATAAAATCTCAGTTAC
ATATGATGGGTTGATTCACGATGTGTCAGTCGGTTCAACGATTTTGCTTGACGATGGATTGGTCGGACTTGAAGTCACTG
ACATAAACAAGGACAAGCGTGAAATCGTCACGAAAGTGATGAACAGCGGAACGTTGAAAAACAAAAAAGGCGTGAATGTG
CCGGGCGTCAGCGTCAATCTTCCGGGAATTACGGAAAAAGATGCAAACGACATCGTATTCGGCATCGAGCAGGGCGTTGA
TTTCATCGCGGCATCCTTTGTGCGCCGTCCGTCGGATGTCCTTGAAATCCGCGAACTTCTTGAAGAGCACAATGCGGCAG
ATATTCAAATCATTCCAAAAATCGAAAACCAGGAAGGCGTTGACAACATCGATGCCATTCTTGAAGTATCAGACGGTCTG
ATGGTTGCGCGTGGAGACCTGGGTGTAGAAATCCCTGCCGAAGAAGTGCCGCTCGTTCAAAAAGAACTGATCAAAAAATG
CAATGCGCTTGGCAAGCCTGTCATCACGGCTACCCAAATGCTCGACAGCATGCAGCGCAATCCGCGCCCGACACGCGCGG
AAGCGAGCGACGTTGCCAATGCGATCTTTGACGGAACTGACGCAATCATGCTTTCAGGTGAAACGGCAGCGGGAAATTAT
CCTGTTGAAGCCGTTCAGACGATGCACAATATCGCTTCCCGTTCAGAAGAAGCGCTCAACCATAAAAAGATCCTTTCCGC
ACGAAGCAAGCAAGTCAGCATGTCAATTACGGATGCGATCGGCCAATCTGTGGCGCATACGGCAATCAACCTTGATGTGA
ATGCCATCGTCACACCGACGGAAAGCGGCCATACGGCGAGAATGATTTCCAAATATCGCCCGCAGGCGCCGATTGTCGCA
GTTACGGTAAACGATGCGGTGTCAAGAAAGCTGTCACTTGTGTTCGGGGTTTTCGCAACAAGCGGACAAAACCACAGCTC
CACTGACGAAATGCTTGAAAAAGCGGTGCAAAAATCTCTCGATACAGGCATCGTACGCCACGGCGACTTGATCGTTATCA
CGGCCGGAGCTGTCGGTGAAGCCGGTACAACAAACTTGATGAAAGTGTATGTTGTCGGAGACGTGGTCGCAAAAGGCCAG
GGCATCGGACGCAAATCCGCATTTGGTGAAGTGGTCATCGCTCAAAACGCACAAGAAGCGGCCAAAAAAATGAAAGATGG
CGCCGTTTTAGTTACAAAAAGCACGGATCGTGACATGATGGCATCCCTTGAAAAAGCGGCTGCGCTGATTACGGAAGAAG
GCGGCTTAACAAGCCACGCGGCAGTCGTAGGCTTAAGCCTCGGCATCCCTGTCATCGTCGGAATGGAAAATGCGACTTCC
ATTCTGAAAGAGGGAGAAGACATCACGGTCGACTCCGCCCGCGGAGCTGTATACAAAGGACGTGCAAGCGTGCTTTAA

Upstream 100 bases:

>100_bases
TATCATTGAAATACTCGAGCAAAAACATACAATTGATCAAAGCATGTACCGCTTGTCTCAAGAACTGTCAATCTAGTGTA
AAACCCGAGGAGGAAGCAAT

Downstream 100 bases:

>100_bases
TCGCTTATTGAAGAAGAAGGGAAGCTTTCCCCTTCTTTTAGATTTATGACAAAATCCTAAAACGTTTTTCAGTTTTAGGA
TTTTGTCATCCTTCAATTCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MRKTKIVCTIGPASESVEKLTQLMEAGMNVARLNFSHGDFEEHGARIKNIREAAGKLGKDIGILLDTKGPEIRTHTMENG
SIELAAGSQLIVSMDEVIGTPDKISVTYDGLIHDVSVGSTILLDDGLVGLEVTDINKDKREIVTKVMNSGTLKNKKGVNV
PGVSVNLPGITEKDANDIVFGIEQGVDFIAASFVRRPSDVLEIRELLEEHNAADIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGNY
PVEAVQTMHNIASRSEEALNHKKILSARSKQVSMSITDAIGQSVAHTAINLDVNAIVTPTESGHTARMISKYRPQAPIVA
VTVNDAVSRKLSLVFGVFATSGQNHSSTDEMLEKAVQKSLDTGIVRHGDLIVITAGAVGEAGTTNLMKVYVVGDVVAKGQ
GIGRKSAFGEVVIAQNAQEAAKKMKDGAVLVTKSTDRDMMASLEKAAALITEEGGLTSHAAVVGLSLGIPVIVGMENATS
ILKEGEDITVDSARGAVYKGRASVL

Sequences:

>Translated_585_residues
MRKTKIVCTIGPASESVEKLTQLMEAGMNVARLNFSHGDFEEHGARIKNIREAAGKLGKDIGILLDTKGPEIRTHTMENG
SIELAAGSQLIVSMDEVIGTPDKISVTYDGLIHDVSVGSTILLDDGLVGLEVTDINKDKREIVTKVMNSGTLKNKKGVNV
PGVSVNLPGITEKDANDIVFGIEQGVDFIAASFVRRPSDVLEIRELLEEHNAADIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGNY
PVEAVQTMHNIASRSEEALNHKKILSARSKQVSMSITDAIGQSVAHTAINLDVNAIVTPTESGHTARMISKYRPQAPIVA
VTVNDAVSRKLSLVFGVFATSGQNHSSTDEMLEKAVQKSLDTGIVRHGDLIVITAGAVGEAGTTNLMKVYVVGDVVAKGQ
GIGRKSAFGEVVIAQNAQEAAKKMKDGAVLVTKSTDRDMMASLEKAAALITEEGGLTSHAAVVGLSLGIPVIVGMENATS
ILKEGEDITVDSARGAVYKGRASVL
>Mature_585_residues
MRKTKIVCTIGPASESVEKLTQLMEAGMNVARLNFSHGDFEEHGARIKNIREAAGKLGKDIGILLDTKGPEIRTHTMENG
SIELAAGSQLIVSMDEVIGTPDKISVTYDGLIHDVSVGSTILLDDGLVGLEVTDINKDKREIVTKVMNSGTLKNKKGVNV
PGVSVNLPGITEKDANDIVFGIEQGVDFIAASFVRRPSDVLEIRELLEEHNAADIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGNY
PVEAVQTMHNIASRSEEALNHKKILSARSKQVSMSITDAIGQSVAHTAINLDVNAIVTPTESGHTARMISKYRPQAPIVA
VTVNDAVSRKLSLVFGVFATSGQNHSSTDEMLEKAVQKSLDTGIVRHGDLIVITAGAVGEAGTTNLMKVYVVGDVVAKGQ
GIGRKSAFGEVVIAQNAQEAAKKMKDGAVLVTKSTDRDMMASLEKAAALITEEGGLTSHAAVVGLSLGIPVIVGMENATS
ILKEGEDITVDSARGAVYKGRASVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family

Homologues:

Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=45.3061224489796, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=45.3061224489796, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=44.806517311609, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=44.806517311609, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=44.6028513238289, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=49.3103448275862, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=49.3103448275862, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=45.7983193277311, Blast_Score=199, Evalue=9e-51,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=45.7983193277311, Blast_Score=199, Evalue=9e-51,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=43.778801843318, Blast_Score=169, Evalue=9e-42,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=52.3206751054852, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1788160, Length=484, Percent_Identity=39.2561983471074, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI1787994, Length=233, Percent_Identity=27.4678111587983, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=45.979381443299, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=45.5852156057495, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=45.5852156057495, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=45.5852156057495, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=45.5852156057495, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=42.9158110882957, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=42.1810699588477, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=42.9735234215886, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=42.9735234215886, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=44.963144963145, Blast_Score=335, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=33.8144329896907, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24646914, Length=253, Percent_Identity=45.4545454545455, Blast_Score=209, Evalue=5e-54,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): KPYK_BACLI (P51181)

Other databases:

- EMBL:   D31955
- PIR:   JC4220
- ProteinModelPortal:   P51181
- SMR:   P51181
- PhylomeDB:   P51181
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C; SSF52009 PEP_mobile; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 61944; Mature: 61944

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 32-32 BINDING 245-245 BINDING 246-246 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVCTIGPASESVEKLTQLMEAGMNVARLNFSHGDFEEHGARIKNIREAAGKLGKD
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
IGILLDTKGPEIRTHTMENGSIELAAGSQLIVSMDEVIGTPDKISVTYDGLIHDVSVGST
EEEEEECCCCCEEEEEECCCEEEEECCCEEEEEHHHHHCCCCEEEEEECCEEEEECCCCE
ILLDDGLVGLEVTDINKDKREIVTKVMNSGTLKNKKGVNVPGVSVNLPGITEKDANDIVF
EEEECCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GIEQGVDFIAASFVRRPSDVLEIRELLEEHNAADIQIIPKIENQEGVDNIDAILEVSDGL
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHEECCCE
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGNYPVEAVQTMHNIASRSEEALNHKKILSARSKQVSMSITDAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHH
GQSVAHTAINLDVNAIVTPTESGHTARMISKYRPQAPIVAVTVNDAVSRKLSLVFGVFAT
HHHHHHHEEEEEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCC
SGQNHSSTDEMLEKAVQKSLDTGIVRHGDLIVITAGAVGEAGTTNLMKVYVVGDVVAKGQ
CCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCEEEEEEEEEHHHCCC
GIGRKSAFGEVVIAQNAQEAAKKMKDGAVLVTKSTDRDMMASLEKAAALITEEGGLTSHA
CCCCCCCCCCEEEECCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHH
AVVGLSLGIPVIVGMENATSILKEGEDITVDSARGAVYKGRASVL
HHHEEECCCEEEEECCHHHHHHHCCCCEEEECCCCCEECCCCCCC
>Mature Secondary Structure
MRKTKIVCTIGPASESVEKLTQLMEAGMNVARLNFSHGDFEEHGARIKNIREAAGKLGKD
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
IGILLDTKGPEIRTHTMENGSIELAAGSQLIVSMDEVIGTPDKISVTYDGLIHDVSVGST
EEEEEECCCCCEEEEEECCCEEEEECCCEEEEEHHHHHCCCCEEEEEECCEEEEECCCCE
ILLDDGLVGLEVTDINKDKREIVTKVMNSGTLKNKKGVNVPGVSVNLPGITEKDANDIVF
EEEECCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GIEQGVDFIAASFVRRPSDVLEIRELLEEHNAADIQIIPKIENQEGVDNIDAILEVSDGL
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHEECCCE
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGNYPVEAVQTMHNIASRSEEALNHKKILSARSKQVSMSITDAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHH
GQSVAHTAINLDVNAIVTPTESGHTARMISKYRPQAPIVAVTVNDAVSRKLSLVFGVFAT
HHHHHHHEEEEEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCC
SGQNHSSTDEMLEKAVQKSLDTGIVRHGDLIVITAGAVGEAGTTNLMKVYVVGDVVAKGQ
CCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCEEEEEEEEEHHHCCC
GIGRKSAFGEVVIAQNAQEAAKKMKDGAVLVTKSTDRDMMASLEKAAALITEEGGLTSHA
CCCCCCCCCCEEEECCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHH
AVVGLSLGIPVIVGMENATSILKEGEDITVDSARGAVYKGRASVL
HHHEEECCCEEEEECCHHHHHHHCCCCEEEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104