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The map label for this gene is mdh [H]

Identifier: 52786789

GI number: 52786789

Start: 2946020

End: 2946958

Strand: Reverse

Name: mdh [H]

Synonym: BLi03060

Alternate gene names: 52786789

Gene position: 2946958-2946020 (Counterclockwise)

Preceding gene: 52786790

Following gene: 52786788

Centisome position: 69.79

GC content: 48.35

Gene sequence:

>939_bases
ATGGGAAAGAAGCGAAATAAAGTATCTGTCATCGGAGCGGGTTTTACAGGTGCAACAACTGCGTTTTTAACGGCTCAAAA
AGAATTGGCTGATGTCGTGCTAGTCGACATTCCGCAGCTTGAAAACCCTACAAAAGGGAAAGCGCTCGATATGCTGGAAG
CGAGCCCTGTCCAAGGATTTGACGCGAATATTACAGGAACGGCAAACTATGAAGATACGGCTGGTTCAGATATCGTCGTC
ATCACGGCGGGGATCGCAAGAAAGCCGGGAATGAGCCGGGACGACCTCGTGGCTACAAATGAAAAGATCATGAGGAGCGT
AACGAAGGAAGTTGTTAAATACTCACCTGATTGTATCATCATCGTTCTGACAAACCCTGTAGATGCGATGACATACGCCG
TCTATAAAGAATCGGGCTTCCCGAAAGAGCGGGTCATCGGCCAGTCGGGAATTTTGGATACGGCAAGATTCAGAACGTTT
GTCGCTCAGGAGCTCAACCTTTCCGTCAAAGACATTACAGGTTTTGTACTGGGCGGACACGGGGACGATATGGTTCCGCT
CGTTCGCTACTCTTATGCAGGCGGCATTCCGCTTGAAACGCTTCTTCCGAAAGACCGGATCGATGCGATTGTTGAAAGAA
CGAGAAAAGGCGGGGGAGAAATTGTCAACCTGTTAGGAAACGGAAGCGCGTACTATGCGCCTGCCGCTTCCCTGACAGAA
ATGGTTGAAGCAATCTTAAAAGACCAGCGCCGCGTGCTGCCGACCATTGCCTACCTTGAAGGCGAATACGGCTATGAAGG
CATTTATCTCGGCGTTCCGACGATTATCGGCGGAAACGGTCTTGAACAGATCATTGAACTTGAACTGACTGAAACCGAAA
AAAGTCAGCTCGATAAATCCGTTGAATCAGTCAAAAACGTCATGAAAGTATTATCATAA

Upstream 100 bases:

>100_bases
TGAAGTCAAATGCTCTGAATTCGGTGATGAGCTTATCAAAAACATGTCTTAAACGGCTGTTTTGGGGTAAAACAAACAAC
AATTAAAAGGGGTAGAAAAA

Downstream 100 bases:

>100_bases
TAAACACGGCAGGAAGGCTTTATACAAGCCTTCCTGTTTTTGAATGCGAACCTTTTACATAAGACGGTATGAAAATCAAG
ACATAAACACGGGCTGTCAG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGKKRNKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANITGTANYEDTAGSDIVV
ITAGIARKPGMSRDDLVATNEKIMRSVTKEVVKYSPDCIIIVLTNPVDAMTYAVYKESGFPKERVIGQSGILDTARFRTF
VAQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLLPKDRIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIIGGNGLEQIIELELTETEKSQLDKSVESVKNVMKVLS

Sequences:

>Translated_312_residues
MGKKRNKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANITGTANYEDTAGSDIVV
ITAGIARKPGMSRDDLVATNEKIMRSVTKEVVKYSPDCIIIVLTNPVDAMTYAVYKESGFPKERVIGQSGILDTARFRTF
VAQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLLPKDRIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIIGGNGLEQIIELELTETEKSQLDKSVESVKNVMKVLS
>Mature_311_residues
GKKRNKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANITGTANYEDTAGSDIVVI
TAGIARKPGMSRDDLVATNEKIMRSVTKEVVKYSPDCIIIVLTNPVDAMTYAVYKESGFPKERVIGQSGILDTARFRTFV
AQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLLPKDRIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEM
VEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIIGGNGLEQIIELELTETEKSQLDKSVESVKNVMKVLS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=37.2239747634069, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=37.2239747634069, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=35.4430379746835, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=35.4430379746835, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI15082234, Length=317, Percent_Identity=35.0157728706625, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI291575128, Length=316, Percent_Identity=35.4430379746835, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI4557032, Length=316, Percent_Identity=35.4430379746835, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI9257228, Length=316, Percent_Identity=35.4430379746835, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI4504973, Length=316, Percent_Identity=35.4430379746835, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=37.8995433789954, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=40, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI207028494, Length=198, Percent_Identity=33.8383838383838, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI21735621, Length=319, Percent_Identity=28.5266457680251, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI103472011, Length=316, Percent_Identity=25.6329113924051, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI103472015, Length=186, Percent_Identity=29.0322580645161, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1789632, Length=316, Percent_Identity=30.379746835443, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535107, Length=300, Percent_Identity=31.3333333333333, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17554310, Length=323, Percent_Identity=30.030959752322, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=294, Percent_Identity=28.9115646258503, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=298, Percent_Identity=26.8456375838926, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136226, Length=313, Percent_Identity=31.3099041533546, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45550422, Length=303, Percent_Identity=29.3729372937294, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24647881, Length=326, Percent_Identity=29.1411042944785, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24663599, Length=234, Percent_Identity=29.9145299145299, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24663595, Length=268, Percent_Identity=28.3582089552239, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33597; Mature: 33466

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKRNKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANITGTANYEDTAGSDIVVITAGIARKPGMSRDDLVATNEKIMRSVTKEVVKYSPDCII
CCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
IVLTNPVDAMTYAVYKESGFPKERVIGQSGILDTARFRTFVAQELNLSVKDITGFVLGGH
EEECCCHHHHHEEEHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCC
GDDMVPLVRYSYAGGIPLETLLPKDRIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
CCCCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIIGGNGLEQIIELELTETEKSQLDKS
HHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCEEECCCCHHHHHEECCCHHHHHHHHHH
VESVKNVMKVLS
HHHHHHHHHHHC
>Mature Secondary Structure 
GKKRNKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANITGTANYEDTAGSDIVVITAGIARKPGMSRDDLVATNEKIMRSVTKEVVKYSPDCII
CCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
IVLTNPVDAMTYAVYKESGFPKERVIGQSGILDTARFRTFVAQELNLSVKDITGFVLGGH
EEECCCHHHHHEEEHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCC
GDDMVPLVRYSYAGGIPLETLLPKDRIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
CCCCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIIGGNGLEQIIELELTETEKSQLDKS
HHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCEEECCCCHHHHHEECCCHHHHHHHHHH
VESVKNVMKVLS
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA