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The map label for this gene is racE [H]

Identifier: 52786718

GI number: 52786718

Start: 2869634

End: 2870452

Strand: Reverse

Name: racE [H]

Synonym: BLi02987

Alternate gene names: 52786718

Gene position: 2870452-2869634 (Counterclockwise)

Preceding gene: 52786719

Following gene: 52786717

Centisome position: 67.98

GC content: 47.5

Gene sequence:

>819_bases
TTGTTGGATCAACCGATAGGAGTCATTGATTCGGGAGTCGGCGGTTTAACCGTTGCAAAAGAAATCATGAGGCAGCTGCC
GAAAGAAAAAATTATATATCTTGGAGACACGAAAAGGTGTCCATACGGACCGAGGGACAGAAAAGAAGTCCTGTCATATA
CATGGGAAATGACAAATTATCTGTTATCCCGCCACCATATTAAAATGCTCGTGATCGCCTGCAATACAGCAACGGCCATC
GCGCTAGAGGAAATCAGCAGGGAAGTTGCGATACCCGTCATCGGCGTGGTGCAGCCCGGAGCCAGAACCGCGATTAAAGT
GACGGAAAACCAGCGAATCGGCGTAATCGGAACGGAAAACACGATCAAAAGCGGCGCATACGAAGAAGCGCTTCTTTCGC
TGAACGGAGATTTAACGGTGGAAAATCTGGCTTGCCCAATGTTTGTCCCGTTTGTGGAAAGCGGCAAATTTTTGGATGAG
ACGGCTGATGAAATCGTCAGGACGTCACTGCACCCGCTCAGAGATTCATCAATCGATACGCTGATTCTCGGATGTACGCA
TTATCCGATTTTAAAAGAGCCGATTCAGCGCTATATGGGAGAGCGCGTCAACATCATTTCCTCCGGAGATGAAACGGCCA
GGGAAGTCAGCACGATCTTGTCATACAACGGTTTATTAAGCAAAACGGCGGAACCGCCGGAACACTTGTTTTTAACAACT
GGGGAGCGTGCCCGCTTCACAAAAATTGCAAACGATTGGTTTGGATACGAAGTGAAAAATGTGGAAAGCATTTCTCTTCA
GGAGACCGCTGTAAAATAA

Upstream 100 bases:

>100_bases
TTAGAAGACATGTTAGAGGCGTTTTCCGAGGAAGAAACAGCCGCGTTTGAAAAATTATTAATTAAACTTCAGCAGGAAAT
GAACAGAAAATGAGGCGATT

Downstream 100 bases:

>100_bases
AGCTGCCGCACATGCAAACGGCAGCTTTTTTCATGGCCTTTTCTTTTAAAGAAAAGGTTTTTTGTATTCAGAGGGATTTT
TTTAAAAAGCGGTCTAATTC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MLDQPIGVIDSGVGGLTVAKEIMRQLPKEKIIYLGDTKRCPYGPRDRKEVLSYTWEMTNYLLSRHHIKMLVIACNTATAI
ALEEISREVAIPVIGVVQPGARTAIKVTENQRIGVIGTENTIKSGAYEEALLSLNGDLTVENLACPMFVPFVESGKFLDE
TADEIVRTSLHPLRDSSIDTLILGCTHYPILKEPIQRYMGERVNIISSGDETAREVSTILSYNGLLSKTAEPPEHLFLTT
GERARFTKIANDWFGYEVKNVESISLQETAVK

Sequences:

>Translated_272_residues
MLDQPIGVIDSGVGGLTVAKEIMRQLPKEKIIYLGDTKRCPYGPRDRKEVLSYTWEMTNYLLSRHHIKMLVIACNTATAI
ALEEISREVAIPVIGVVQPGARTAIKVTENQRIGVIGTENTIKSGAYEEALLSLNGDLTVENLACPMFVPFVESGKFLDE
TADEIVRTSLHPLRDSSIDTLILGCTHYPILKEPIQRYMGERVNIISSGDETAREVSTILSYNGLLSKTAEPPEHLFLTT
GERARFTKIANDWFGYEVKNVESISLQETAVK
>Mature_272_residues
MLDQPIGVIDSGVGGLTVAKEIMRQLPKEKIIYLGDTKRCPYGPRDRKEVLSYTWEMTNYLLSRHHIKMLVIACNTATAI
ALEEISREVAIPVIGVVQPGARTAIKVTENQRIGVIGTENTIKSGAYEEALLSLNGDLTVENLACPMFVPFVESGKFLDE
TADEIVRTSLHPLRDSSIDTLILGCTHYPILKEPIQRYMGERVNIISSGDETAREVSTILSYNGLLSKTAEPPEHLFLTT
GERARFTKIANDWFGYEVKNVESISLQETAVK

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=226, Percent_Identity=29.646017699115, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 30182; Mature: 30182

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQPIGVIDSGVGGLTVAKEIMRQLPKEKIIYLGDTKRCPYGPRDRKEVLSYTWEMTNY
CCCCCCCHHHCCCCHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LLSRHHIKMLVIACNTATAIALEEISREVAIPVIGVVQPGARTAIKVTENQRIGVIGTEN
HHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCEEEEEECCC
TIKSGAYEEALLSLNGDLTVENLACPMFVPFVESGKFLDETADEIVRTSLHPLRDSSIDT
CCCCCCHHHHHHHCCCCEEEECCCCCEEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCE
LILGCTHYPILKEPIQRYMGERVNIISSGDETAREVSTILSYNGLLSKTAEPPEHLFLTT
EEEECCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEC
GERARFTKIANDWFGYEVKNVESISLQETAVK
CCCHHHHHHHHHHCCEEECCCCEEEHHHHHCC
>Mature Secondary Structure
MLDQPIGVIDSGVGGLTVAKEIMRQLPKEKIIYLGDTKRCPYGPRDRKEVLSYTWEMTNY
CCCCCCCHHHCCCCHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LLSRHHIKMLVIACNTATAIALEEISREVAIPVIGVVQPGARTAIKVTENQRIGVIGTEN
HHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCEEEEEECCC
TIKSGAYEEALLSLNGDLTVENLACPMFVPFVESGKFLDETADEIVRTSLHPLRDSSIDT
CCCCCCHHHHHHHCCCCEEEECCCCCEEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCE
LILGCTHYPILKEPIQRYMGERVNIISSGDETAREVSTILSYNGLLSKTAEPPEHLFLTT
EEEECCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEC
GERARFTKIANDWFGYEVKNVESISLQETAVK
CCCHHHHHHHHHHCCEEECCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9063446; 8969504; 9384377; 9604005 [H]