Click here to switch to the map view.

The map label for this gene is fnuDIM [H]

Identifier: 52786706

GI number: 52786706

Start: 2859764

End: 2860534

Strand: Reverse

Name: fnuDIM [H]

Synonym: BLi02974

Alternate gene names: 52786706

Gene position: 2860534-2859764 (Counterclockwise)

Preceding gene: 52786707

Following gene: 52786705

Centisome position: 67.74

GC content: 42.41

Gene sequence:

>771_bases
ATGGAAAAGAAAGAGTACCGTTGGAAGTTGGGCGATTTAAAAAACATCAAGAAAAATGGCCTTAAAGTATTCAGTACATT
CTCTTGGGGCGGGGGTTCCTCTATGGGCTATAAGCTCGCCGGTTACGATCTTTTGGGGAACTGTGAGATCGATCCGCAAA
TGATGAAGATTTACAGGAAGAATCATAATCCGATATATCCTTTTCTTATGGACATCAGAGACTTTAACAAAATGGAAAAT
CTGCCTGATGAATTGTTCGATTTAGATATTTTTGATGGTTCCCCGCCCTGCAGCGTGTTTTCTATTGCGGGGGATCGGGA
AAAGGCTTGGGGAAAAGAAAAAGCCTTTCGTAAAGGACAGGCAAAACAGTCACTTGATGATCTATTTTTTCATTACCTTG
ATGCTGTTGAAAGGCTGCGGCCAAAAATCTTTGTGGCCGAGAATGTCAAAGGAATGGTCAGCGGAAATGCAAAAGGTTAT
GTGAAGCTGGTGATTGAACGGACCAAGGAAATTGGATATGATGTTCAACTGTTCTTGTTGAATGCTGCCACAATGGGAGC
CCCGCAACGTAGAGAGCGGGTCTTTTTTATTTGCCGAAGGAAGGACTTGAATTTACTACCGCTTAAATTGTCCTTCAATG
AGCCACCGATTGTATATAAAGAATTCAGAAGCGGCCATGGTGCAAGGCTTAAAGAAACATCAAAGCTTTATAAAAGATGG
GTGAAGCGACGGCCGTCGGATAATAATGGGAGACATCACGAAAAGGATTGA

Upstream 100 bases:

>100_bases
TCAATGACAGCCAGGAATTAGACCTGGATGATTATGAAGATGATCATTTCCAACATACCTGCCCCAAATGCGGATTTTCA
TTTAATGAATAGATGATTAC

Downstream 100 bases:

>100_bases
AGGGAAAGAATCTAATTTCAATACGATATTAGTGAAAAATAGCCTTGTCCCGCCTACTCTCGGGAGTGGTTCGGTATTTG
TCCGTTATGATGAGCCCTAC

Product: hypothetical protein

Products: NA

Alternate protein names: M.FnuDI; Cytosine-specific methyltransferase FnuDI [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MEKKEYRWKLGDLKNIKKNGLKVFSTFSWGGGSSMGYKLAGYDLLGNCEIDPQMMKIYRKNHNPIYPFLMDIRDFNKMEN
LPDELFDLDIFDGSPPCSVFSIAGDREKAWGKEKAFRKGQAKQSLDDLFFHYLDAVERLRPKIFVAENVKGMVSGNAKGY
VKLVIERTKEIGYDVQLFLLNAATMGAPQRRERVFFICRRKDLNLLPLKLSFNEPPIVYKEFRSGHGARLKETSKLYKRW
VKRRPSDNNGRHHEKD

Sequences:

>Translated_256_residues
MEKKEYRWKLGDLKNIKKNGLKVFSTFSWGGGSSMGYKLAGYDLLGNCEIDPQMMKIYRKNHNPIYPFLMDIRDFNKMEN
LPDELFDLDIFDGSPPCSVFSIAGDREKAWGKEKAFRKGQAKQSLDDLFFHYLDAVERLRPKIFVAENVKGMVSGNAKGY
VKLVIERTKEIGYDVQLFLLNAATMGAPQRRERVFFICRRKDLNLLPLKLSFNEPPIVYKEFRSGHGARLKETSKLYKRW
VKRRPSDNNGRHHEKD
>Mature_256_residues
MEKKEYRWKLGDLKNIKKNGLKVFSTFSWGGGSSMGYKLAGYDLLGNCEIDPQMMKIYRKNHNPIYPFLMDIRDFNKMEN
LPDELFDLDIFDGSPPCSVFSIAGDREKAWGKEKAFRKGQAKQSLDDLFFHYLDAVERLRPKIFVAENVKGMVSGNAKGY
VKLVIERTKEIGYDVQLFLLNAATMGAPQRRERVFFICRRKDLNLLPLKLSFNEPPIVYKEFRSGHGARLKETSKLYKRW
VKRRPSDNNGRHHEKD

Specific function: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the FnuDI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 29812; Mature: 29812

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKEYRWKLGDLKNIKKNGLKVFSTFSWGGGSSMGYKLAGYDLLGNCEIDPQMMKIYRK
CCCCCCEECCCCHHHHHHCCEEEEEEECCCCCCCCCEEEECCEEECCCCCCHHHHHHHHH
NHNPIYPFLMDIRDFNKMENLPDELFDLDIFDGSPPCSVFSIAGDREKAWGKEKAFRKGQ
CCCCCCHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCEEEEECCCCHHHCCHHHHHHCCC
AKQSLDDLFFHYLDAVERLRPKIFVAENVKGMVSGNAKGYVKLVIERTKEIGYDVQLFLL
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCEEHHEEHHHHHCCCCEEEEEE
NAATMGAPQRRERVFFICRRKDLNLLPLKLSFNEPPIVYKEFRSGHGARLKETSKLYKRW
EHHHCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHH
VKRRPSDNNGRHHEKD
HHCCCCCCCCCCCCCC
>Mature Secondary Structure
MEKKEYRWKLGDLKNIKKNGLKVFSTFSWGGGSSMGYKLAGYDLLGNCEIDPQMMKIYRK
CCCCCCEECCCCHHHHHHCCEEEEEEECCCCCCCCCEEEECCEEECCCCCCHHHHHHHHH
NHNPIYPFLMDIRDFNKMENLPDELFDLDIFDGSPPCSVFSIAGDREKAWGKEKAFRKGQ
CCCCCCHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCEEEEECCCCHHHCCHHHHHHCCC
AKQSLDDLFFHYLDAVERLRPKIFVAENVKGMVSGNAKGYVKLVIERTKEIGYDVQLFLL
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCEEHHEEHHHHHCCCCEEEEEE
NAATMGAPQRRERVFFICRRKDLNLLPLKLSFNEPPIVYKEFRSGHGARLKETSKLYKRW
EHHHCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHH
VKRRPSDNNGRHHEKD
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA