Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 52786693

GI number: 52786693

Start: 2849066

End: 2850790

Strand: Reverse

Name: ilvB [H]

Synonym: BLi02961

Alternate gene names: 52786693

Gene position: 2850790-2849066 (Counterclockwise)

Preceding gene: 52786694

Following gene: 52786692

Centisome position: 67.51

GC content: 50.9

Gene sequence:

>1725_bases
ATGGGGACGAATGTACAGTTGGATGCTGAAAATGCCCAATGTACACAAACAATGGCAGGGGCGTCAATGCTGATCGAGGC
TTTAAAACAGGAAAATGTTGAAGTGATCTACGGGTATCCGGGCGGTGCGGTCCTGCCGATTTATGACAAACTCTACGGAT
CGGGAATGTTTCATGTGCTGACGCGCCATGAACAAGGCGCCATCCACGCAGCCGAAGGATATGCAAGGATTTCCGGGAAA
CCGGGTGTCGTCATCGCTACATCCGGACCGGGAGCGACCAACCTTGTAACGGGTCTGGCCGACGCGATGATCGATTCATT
GCCTCTCGTCGTATTCACCGGTCAGGTTGCGACATCGGTTATCGGATCTGACGCATTCCAAGAAGCGGACGTCATCGGAA
TTACGATGCCGATTACAAAGCACAGCTACCAGGTACGCAATGCCGAAGATTTGCCGAGAATTATTAAAGAAGCATTCCAT
ATCGCAACAACAGGAAGACCGGGCCCCGTTTTAATCGATATTCCTAAAGACATCGCCGTTGTAGAAGGAGAATTCCGCTA
TGACCACGACATTCACCTTCCGGGCTATCAGCCGACGAAGGAACCGAATTATCTGCAGATCAGAAAGCTTGTGGAAGCGG
TCAGTGCAGCGAAAAAGCCGGTTATTTTAGCGGGTGCGGGCGTCTTGCACGGGAAAGCGTCAGAAGAATTAAAAAATTAT
GTCGAACAGCAGCAGATTCCGGTGGCACATACGCTGTTAGGCTTGGGAGGATTTCCGGCCGATCATCCGCTTTTCCTCGG
GATGGCCGGAATGCACGGAACGTATGCGGCCAACATGGCTCTGTATGAAAGCGATCTGTTAATCAGCATCGGTGCCAGGT
TTGATGACCGGGTCACAGGGAACTTGAAGCATTTTGCAAAATATGCGAAAGTCGCTCACATTGACATCGATCCGGCTGAA
ATCGGCAAAAACGTTCATACGCACATTCCGGTTGTCGGCGACAGCAAGCTTGTACTGACGGAGCTGATCAAGCAAAACGG
CAAGCCGAGCGCTTCTGATGAGTGGAAAAAGCAGCTTGCGGCATGGAAAACCGAATATCCGCTCTGGTATGAAGAAAATG
AAACAGAAGGATTCAAACCGCAGAAGCTGATCGAATATATCCACCGCTATACAAAGGGCGAAGCTATTGTAACAACAGAC
GTCGGACAGCACCAAATGTGGGCCGCGCAATTTTATCAGTTCCAAAAAGCCGACAGATGGGTCACTTCCGGCGGGCTCGG
CACAATGGGCTTCGGACTTCCGGCGGCAATCGGAGCACAGCTTGCGGATAAGGATGCGACGGTTGTATCGATACTCGGCG
ACGGAGGATTCCAGATGACGCTGCAGGAGCTGTCTGTTATTCACGATCTGAACCTTCCGATCAAGGTCGTGGTCTTAAAC
AACCGCTGCCTCGGAATGGTCCGGCAATGGCAGGAAATCTTTTACGATGAGCGCTATTCGCACTCCAAGTTCACATCACA
GCCTGATTTCATCAAGCTGTCTGAAGCTTACGGCATCAAAGGCGTCCGAATCACATCCGAAGAAGAAGCGGAAGAAAAGC
TGGAAGCGGCGCTAACGTCGAATGAACCGATGGTCATTGATGTTCAAGTTGCACAAGCTGAAAAAGTATTTCCAATGGTT
GCGCCTGGAAAAGGGCTGCACGAAATGGTGGGGGTGAAACCTTGA

Upstream 100 bases:

>100_bases
AGATTGGATACATGCTTTGCGGCACATATATATTGATTATTTTTTGAAAAACAATTGTGAATGAACAGTGATGATGATCA
CTTGATAAGGAGGAAGAAAA

Downstream 100 bases:

>100_bases
AAAGAATCATCACGCTGACGGTTTTGAACCAAAGCGGCGTCTTAAACAGAATCACAGGGTTGTTTACAAAACGGCATTAC
AACATTGAAAGCATTACGGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MGTNVQLDAENAQCTQTMAGASMLIEALKQENVEVIYGYPGGAVLPIYDKLYGSGMFHVLTRHEQGAIHAAEGYARISGK
PGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADVIGITMPITKHSYQVRNAEDLPRIIKEAFH
IATTGRPGPVLIDIPKDIAVVEGEFRYDHDIHLPGYQPTKEPNYLQIRKLVEAVSAAKKPVILAGAGVLHGKASEELKNY
VEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYESDLLISIGARFDDRVTGNLKHFAKYAKVAHIDIDPAE
IGKNVHTHIPVVGDSKLVLTELIKQNGKPSASDEWKKQLAAWKTEYPLWYEENETEGFKPQKLIEYIHRYTKGEAIVTTD
VGQHQMWAAQFYQFQKADRWVTSGGLGTMGFGLPAAIGAQLADKDATVVSILGDGGFQMTLQELSVIHDLNLPIKVVVLN
NRCLGMVRQWQEIFYDERYSHSKFTSQPDFIKLSEAYGIKGVRITSEEEAEEKLEAALTSNEPMVIDVQVAQAEKVFPMV
APGKGLHEMVGVKP

Sequences:

>Translated_574_residues
MGTNVQLDAENAQCTQTMAGASMLIEALKQENVEVIYGYPGGAVLPIYDKLYGSGMFHVLTRHEQGAIHAAEGYARISGK
PGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADVIGITMPITKHSYQVRNAEDLPRIIKEAFH
IATTGRPGPVLIDIPKDIAVVEGEFRYDHDIHLPGYQPTKEPNYLQIRKLVEAVSAAKKPVILAGAGVLHGKASEELKNY
VEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYESDLLISIGARFDDRVTGNLKHFAKYAKVAHIDIDPAE
IGKNVHTHIPVVGDSKLVLTELIKQNGKPSASDEWKKQLAAWKTEYPLWYEENETEGFKPQKLIEYIHRYTKGEAIVTTD
VGQHQMWAAQFYQFQKADRWVTSGGLGTMGFGLPAAIGAQLADKDATVVSILGDGGFQMTLQELSVIHDLNLPIKVVVLN
NRCLGMVRQWQEIFYDERYSHSKFTSQPDFIKLSEAYGIKGVRITSEEEAEEKLEAALTSNEPMVIDVQVAQAEKVFPMV
APGKGLHEMVGVKP
>Mature_573_residues
GTNVQLDAENAQCTQTMAGASMLIEALKQENVEVIYGYPGGAVLPIYDKLYGSGMFHVLTRHEQGAIHAAEGYARISGKP
GVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADVIGITMPITKHSYQVRNAEDLPRIIKEAFHI
ATTGRPGPVLIDIPKDIAVVEGEFRYDHDIHLPGYQPTKEPNYLQIRKLVEAVSAAKKPVILAGAGVLHGKASEELKNYV
EQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYESDLLISIGARFDDRVTGNLKHFAKYAKVAHIDIDPAEI
GKNVHTHIPVVGDSKLVLTELIKQNGKPSASDEWKKQLAAWKTEYPLWYEENETEGFKPQKLIEYIHRYTKGEAIVTTDV
GQHQMWAAQFYQFQKADRWVTSGGLGTMGFGLPAAIGAQLADKDATVVSILGDGGFQMTLQELSVIHDLNLPIKVVVLNN
RCLGMVRQWQEIFYDERYSHSKFTSQPDFIKLSEAYGIKGVRITSEEEAEEKLEAALTSNEPMVIDVQVAQAEKVFPMVA
PGKGLHEMVGVKP

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=26.0034904013962, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI21361361, Length=585, Percent_Identity=22.7350427350427, Blast_Score=148, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=47.7031802120141, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1790104, Length=556, Percent_Identity=42.9856115107914, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=34.4569288389513, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=27.859778597786, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=26.173285198556, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=27.0125223613596, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=27.0125223613596, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=521, Percent_Identity=25.1439539347409, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=46.286701208981, Blast_Score=503, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=24.040404040404, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=484, Percent_Identity=25.8264462809917, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=484, Percent_Identity=25, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320123, Length=535, Percent_Identity=22.0560747663551, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=23.859649122807, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62637; Mature: 62506

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTNVQLDAENAQCTQTMAGASMLIEALKQENVEVIYGYPGGAVLPIYDKLYGSGMFHVL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHHCCCEEEEE
TRHEQGAIHAAEGYARISGKPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSV
EECCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHH
IGSDAFQEADVIGITMPITKHSYQVRNAEDLPRIIKEAFHIATTGRPGPVLIDIPKDIAV
HCCCHHCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCEEE
VEGEFRYDHDIHLPGYQPTKEPNYLQIRKLVEAVSAAKKPVILAGAGVLHGKASEELKNY
EECCEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHH
VEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYESDLLISIGARFDDRVTG
HHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCEEEEECCEEEEECCCCCCCCCC
NLKHFAKYAKVAHIDIDPAEIGKNVHTHIPVVGDSKLVLTELIKQNGKPSASDEWKKQLA
CHHHHHHHHHEEEEECCHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHH
AWKTEYPLWYEENETEGFKPQKLIEYIHRYTKGEAIVTTDVGQHQMWAAQFYQFQKADRW
HHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCE
VTSGGLGTMGFGLPAAIGAQLADKDATVVSILGDGGFQMTLQELSVIHDLNLPIKVVVLN
EECCCCCCCCCCCHHHHCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEC
NRCLGMVRQWQEIFYDERYSHSKFTSQPDFIKLSEAYGIKGVRITSEEEAEEKLEAALTS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHCCCCEEEECCHHHHHHHHHHHHCC
NEPMVIDVQVAQAEKVFPMVAPGKGLHEMVGVKP
CCCEEEEEEECCCCCCCEEECCCCCHHHHCCCCC
>Mature Secondary Structure 
GTNVQLDAENAQCTQTMAGASMLIEALKQENVEVIYGYPGGAVLPIYDKLYGSGMFHVL
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHHCCCEEEEE
TRHEQGAIHAAEGYARISGKPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSV
EECCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHH
IGSDAFQEADVIGITMPITKHSYQVRNAEDLPRIIKEAFHIATTGRPGPVLIDIPKDIAV
HCCCHHCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCEEE
VEGEFRYDHDIHLPGYQPTKEPNYLQIRKLVEAVSAAKKPVILAGAGVLHGKASEELKNY
EECCEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHH
VEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYESDLLISIGARFDDRVTG
HHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCEEEEECCEEEEECCCCCCCCCC
NLKHFAKYAKVAHIDIDPAEIGKNVHTHIPVVGDSKLVLTELIKQNGKPSASDEWKKQLA
CHHHHHHHHHEEEEECCHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHH
AWKTEYPLWYEENETEGFKPQKLIEYIHRYTKGEAIVTTDVGQHQMWAAQFYQFQKADRW
HHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCE
VTSGGLGTMGFGLPAAIGAQLADKDATVVSILGDGGFQMTLQELSVIHDLNLPIKVVVLN
EECCCCCCCCCCCHHHHCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEC
NRCLGMVRQWQEIFYDERYSHSKFTSQPDFIKLSEAYGIKGVRITSEEEAEEKLEAALTS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHCCCCEEEECCHHHHHHHHHHHHCC
NEPMVIDVQVAQAEKVFPMVAPGKGLHEMVGVKP
CCCEEEEEEECCCCCCCEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]