Click here to switch to the map view.

The map label for this gene is yqfS [H]

Identifier: 52786428

GI number: 52786428

Start: 2592176

End: 2593069

Strand: Reverse

Name: yqfS [H]

Synonym: BLi02692

Alternate gene names: 52786428

Gene position: 2593069-2592176 (Counterclockwise)

Preceding gene: 52786429

Following gene: 52786426

Centisome position: 61.41

GC content: 46.53

Gene sequence:

>894_bases
TTGTTGAAAATCGGTTCACACGTCTCAATGAGCGGAAAACATATGCTGCTTGCGGCCAGTCAGGAGGCAAGCTCCTACGG
CGCCAATACATTTATGATCTATACGGGAGCGCCGCAGAACACGCGCAGGAAAAAAATCGAGGATTTGAATATTGAAGCGG
GAAGGGCTCACATGGAAGAGAACGGCATCTCTGATATCGTCGTTCATGCCCCGTATATTATCAATATTGCCAATACAACG
AATCCGGCCACATTTGAGCTCGGTGTCGAGTTCTTGCGTTCTGAAATTGAAAGGACGTCGGCGATCGGCGCCAGACAAAT
CGTTCTTCACCCGGGAGCTCATGTAGGCGCAGGCGCAGAAACGGGCATTCAAAAAATCATCGAAGGATTGAACGAGGTCA
TCGACCCGAATCAGAATGTGCAGATCGCTTTGGAAACGATGGCCGGGAAAGGTTCAGAATGCGGGCGGACGTTTGAGGAG
CTTGCACAGATCATCGACGGCGTTACACACAACGAGCATTTATCCGTCTGCTTCGATACATGCCATACCCATGACGCGGG
TTACGATGTAGTATCCGACTTTGACGGTGTCTTAAACGAATTCGATAAAATTGTCGGAATCGACCGTTTAAAAGTGCTTC
ATATTAATGACAGCAAGAATGTCAGAGGTGCGAGAAAGGACCGCCACGAAAACATCGGATTCGGAGAAATCGGTTTTGAT
GCGCTCCAATACATCGTTCACCACGATCAGCTGAAAGATATTCCGAAAATCCTTGAAACGCCTTATGTAGGAGAAGATAA
AAAGAACAAAAAGCCGCCTTACCGGTTTGAAATTGAAATGCTCAAAAATAAACAGTTTGATGAAGAGCTGCTTGAAAAAA
TCAAGCAGCAATAA

Upstream 100 bases:

>100_bases
TCAAGCCTGGATATAAGAAGAAAATGCAGACGGAAATCCAAAAAATAAAAAGACAGCAAAAAAGAAAGCAATTTAGAAAA
GGAAAGTAGGGGAAACAGTT

Downstream 100 bases:

>100_bases
AAAAAGGGGGGCACACCAGCTCCCCTTTTATGATACGTATTGAGCAAACAGCTGGTTCAATAGCTGCACCGCTTCTTTTG
AAGTGACCGCTTCCACTTGC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLKIGSHVSMSGKHMLLAASQEASSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMEENGISDIVVHAPYIINIANTT
NPATFELGVEFLRSEIERTSAIGARQIVLHPGAHVGAGAETGIQKIIEGLNEVIDPNQNVQIALETMAGKGSECGRTFEE
LAQIIDGVTHNEHLSVCFDTCHTHDAGYDVVSDFDGVLNEFDKIVGIDRLKVLHINDSKNVRGARKDRHENIGFGEIGFD
ALQYIVHHDQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKNKQFDEELLEKIKQQ

Sequences:

>Translated_297_residues
MLKIGSHVSMSGKHMLLAASQEASSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMEENGISDIVVHAPYIINIANTT
NPATFELGVEFLRSEIERTSAIGARQIVLHPGAHVGAGAETGIQKIIEGLNEVIDPNQNVQIALETMAGKGSECGRTFEE
LAQIIDGVTHNEHLSVCFDTCHTHDAGYDVVSDFDGVLNEFDKIVGIDRLKVLHINDSKNVRGARKDRHENIGFGEIGFD
ALQYIVHHDQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKNKQFDEELLEKIKQQ
>Mature_297_residues
MLKIGSHVSMSGKHMLLAASQEASSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMEENGISDIVVHAPYIINIANTT
NPATFELGVEFLRSEIERTSAIGARQIVLHPGAHVGAGAETGIQKIIEGLNEVIDPNQNVQIALETMAGKGSECGRTFEE
LAQIIDGVTHNEHLSVCFDTCHTHDAGYDVVSDFDGVLNEFDKIVGIDRLKVLHINDSKNVRGARKDRHENIGFGEIGFD
ALQYIVHHDQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKNKQFDEELLEKIKQQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=265, Percent_Identity=33.9622641509434, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=34.2205323193916, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322735, Length=298, Percent_Identity=29.8657718120805, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33070; Mature: 33070

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGSHVSMSGKHMLLAASQEASSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMEE
CCCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHH
NGISDIVVHAPYIINIANTTNPATFELGVEFLRSEIERTSAIGARQIVLHPGAHVGAGAE
CCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
TGIQKIIEGLNEVIDPNQNVQIALETMAGKGSECGRTFEELAQIIDGVTHNEHLSVCFDT
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
CHTHDAGYDVVSDFDGVLNEFDKIVGIDRLKVLHINDSKNVRGARKDRHENIGFGEIGFD
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHH
ALQYIVHHDQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKNKQFDEELLEKIKQQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLKIGSHVSMSGKHMLLAASQEASSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMEE
CCCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHH
NGISDIVVHAPYIINIANTTNPATFELGVEFLRSEIERTSAIGARQIVLHPGAHVGAGAE
CCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
TGIQKIIEGLNEVIDPNQNVQIALETMAGKGSECGRTFEELAQIIDGVTHNEHLSVCFDT
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
CHTHDAGYDVVSDFDGVLNEFDKIVGIDRLKVLHINDSKNVRGARKDRHENIGFGEIGFD
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHH
ALQYIVHHDQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKNKQFDEELLEKIKQQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA