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The map label for this gene is opuAB [H]

Identifier: 52786365

GI number: 52786365

Start: 2540687

End: 2541541

Strand: Reverse

Name: opuAB [H]

Synonym: BLi02627

Alternate gene names: 52786365

Gene position: 2541541-2540687 (Counterclockwise)

Preceding gene: 52786366

Following gene: 52786364

Centisome position: 60.19

GC content: 48.19

Gene sequence:

>855_bases
ATGTTTACTTTATCATCGCTTCCCCGCATTCCTTTAGCCGATTGGATCGATCAGTTTGTTGACTGGCTGACGGTGTCGTT
CGGCGGTGTATTTAACGGAATTGCCAGCGGAGTGGAATCGTTTGTTGATGAGATTGTCCAAGGGCTGGGTTTTGTTCCGC
CGGTTATGTTGACCGTTATTTTTGCCGCGCTCGTCTGGCTGATCAGCAAAAGAGGCATTGCGCTGTTTACTTTAATCGGA
TTTTTTATCATTGATTATTTAGGATATTGGGACGCCATGCTCCAGACATTGGCGCTTGTCGTCACATCCGTTCTGATTTC
TGTCATTATCGGCATTCCGGTCGGAATCTGGGGCTCCCAAAAGAAAACGGTTCGGAGAGTCGTTACGCCCATCCTTGACT
TAATGCAGACAATGCCGGCATTCGTTTATTTACTTCCGGCCATCTTCTTTTTCAATATCGGGGTTGTGCCGGGTGTTGTC
GCTTCCGTTATCTTTGCGATGCCTCCGACAATCCGGATGACGATCTTAGGGATCCAGCAGGTGCCTGAAGATCTGATTGA
AGCTACAGAAGCATTTGGTTCAACGACAAGCCAGCGGCTCTTTAAAGTGCAGCTGCCGCTCGCGACGAAAACCATTCTGG
CCGGTATCAATCAAAGCATCATGCTTGCTTTGTCAATGGTCGTTATTGCATCAATGGTAGGCGCGCCCGGACTCGGAGAA
GAAGTTTACCGGGCTGTGACCCAGCTGCAAACGGGTACCGGTGTAGAAACCGGACTTGCGATTGTTATTATTGCGATCGT
ACTTGACCGGGTTACACAAAATATAAAATTGAAAAAGAGCAGGGGGAATGCATAA

Upstream 100 bases:

>100_bases
TCATCGTCAAAGGCGCATTAATCGGAGCGCTTGCCGGAAATGACCGGTATATTAATGCTCATGAACCAGCCCATGAAGCT
GTTATTCAGGAGGTGAGATA

Downstream 100 bases:

>100_bases
TGTGGAAAAAGATAGCGGGAATCGGCACTGCAGCGGTACTCACATTGGGTCTGGCTGCCTGCGGAAGCAGCAATAATAAT
GAAAATGCGTCAGTAGGCGA

Product: OpuAB

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MFTLSSLPRIPLADWIDQFVDWLTVSFGGVFNGIASGVESFVDEIVQGLGFVPPVMLTVIFAALVWLISKRGIALFTLIG
FFIIDYLGYWDAMLQTLALVVTSVLISVIIGIPVGIWGSQKKTVRRVVTPILDLMQTMPAFVYLLPAIFFFNIGVVPGVV
ASVIFAMPPTIRMTILGIQQVPEDLIEATEAFGSTTSQRLFKVQLPLATKTILAGINQSIMLALSMVVIASMVGAPGLGE
EVYRAVTQLQTGTGVETGLAIVIIAIVLDRVTQNIKLKKSRGNA

Sequences:

>Translated_284_residues
MFTLSSLPRIPLADWIDQFVDWLTVSFGGVFNGIASGVESFVDEIVQGLGFVPPVMLTVIFAALVWLISKRGIALFTLIG
FFIIDYLGYWDAMLQTLALVVTSVLISVIIGIPVGIWGSQKKTVRRVVTPILDLMQTMPAFVYLLPAIFFFNIGVVPGVV
ASVIFAMPPTIRMTILGIQQVPEDLIEATEAFGSTTSQRLFKVQLPLATKTILAGINQSIMLALSMVVIASMVGAPGLGE
EVYRAVTQLQTGTGVETGLAIVIIAIVLDRVTQNIKLKKSRGNA
>Mature_284_residues
MFTLSSLPRIPLADWIDQFVDWLTVSFGGVFNGIASGVESFVDEIVQGLGFVPPVMLTVIFAALVWLISKRGIALFTLIG
FFIIDYLGYWDAMLQTLALVVTSVLISVIIGIPVGIWGSQKKTVRRVVTPILDLMQTMPAFVYLLPAIFFFNIGVVPGVV
ASVIFAMPPTIRMTILGIQQVPEDLIEATEAFGSTTSQRLFKVQLPLATKTILAGINQSIMLALSMVVIASMVGAPGLGE
EVYRAVTQLQTGTGVETGLAIVIIAIVLDRVTQNIKLKKSRGNA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=275, Percent_Identity=47.6363636363636, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1788451, Length=172, Percent_Identity=30.8139534883721, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788449, Length=182, Percent_Identity=30.2197802197802, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786564, Length=157, Percent_Identity=28.6624203821656, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30673; Mature: 30673

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]