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The map label for this gene is recN [H]

Identifier: 52786333

GI number: 52786333

Start: 2514440

End: 2516170

Strand: Reverse

Name: recN [H]

Synonym: BLi02595

Alternate gene names: 52786333

Gene position: 2516170-2514440 (Counterclockwise)

Preceding gene: 52786334

Following gene: 52786332

Centisome position: 59.59

GC content: 47.49

Gene sequence:

>1731_bases
TTGTTAGCTGAATTATCGATCAAAAACTTTGCGATTATCGAAGAATTAACCGTTTCTTTTGAAAAAGGGCTGACGGTTCT
AACCGGTGAAACAGGTGCCGGAAAATCGATCATCATCGACGCCGTCTCTTTGCTTGTCGGAGGCCGCGGATCGTCCGAAT
ATGTCAGGTATGGCGAAAAAAGGGCCGAATTGGAAGGGCTTTTCCTCCTTGACGGCGGCCATCCGGTTTTTGATTTGTGC
ACAGAGCTGGGGATTGAAGCCTCGGACGACATGATCGTCATGAGAAGAGATATTAACGCAAACGGCAAAAGCATCTGCCG
CGTCAATGGAAAACTTGTCACCATTGCCGCATTGCGGGAAGTCGGCAGACTGCTGCTTGACATTCACGGGCAGCACGACA
ATCAGCTGCTGATGGAGGACGAGCACCATCTTCAGCTTCTGGACAGGTTCGCAGGAGAAGAAATCGAGAATGCTTTAAAA
GCGTATCAGGAAGTTTACAGCCGCTACATGGATGTCATGAAGAAGGTGAAAGAGCTTTCTGAAAGCGAGCAGGAAATGGC
TCACAGGCTCGATTTAATACAGTTCCAGCTTGACGAAATCGAATCAGCAAACCTTGAGCCGAAAGAGGACGAGCTTCTTC
AGGAAGAGCGCCGTCAAATTGCAAATTTCGAAAAAATATACGAAGCGCTGCAAAATGCCTATAATGCGCTTCGCAATGAG
CAGGCCGGGCTTGACTGGGTCGGCATGGCTTCAAGCGAGCTTGAAAACGTCTCAGGCATTAATGAAGGGCTCAATAAAGT
ATCTGAATCGGTAGCCAACGCTTACTACATTCTCGAAGATTCAACCTTTCAGATCAGAAATATGCTGGATGAACTCGAAT
ACGACCCCGAGCGCCTCGATTATATCGAATCGCGTTTAAACGAAATCAAACAGCTGAAACGCAAATACGGCGCTACTGTC
GAAGACATCCAAGCTTATTCGGCTAAGATAGAGGAAGAAATCGACCGGATCGAAAACCGGGACAGCCACTTGCAGGCTTT
AAAATCACGACTCGAATCAATCGGCAAGGATGTGGCGATTGAAGCAGCCAATGTGTCCGAAATCAGAAAAAAATGGGCGG
AAAAGCTGGCCAAACAGATTCAGCAGGAATTGAAAGATCTGTATATGGAAAAATCGACATTTGATACGGAATTTCATGTG
AAAAAAGCGGAACGGGATGATGATGCACCCGTCGTCAACGGAATGCCTGTTCAGCTCACAAAGCACGGCATTGACACCGT
TCGTTTCCTGATCTCCACAAACACGGGAGAACCGCTTAAATCCTTGTCAAAGGTTGCTTCAGGAGGAGAGCTCTCAAGAA
TCATGCTGGCGATGAAAAGCATTTTCTCCGCTCAGCAGGATGTTACATCGATCATTTTCGATGAAGTGGACACTGGTGTA
AGCGGAAGAGTCGCGCAGGCGATCGCTGAAAAAATACACCGCGTCTCTATCGGCTCTCAAGTTTTATGCATTACCCACTT
GCCGCAGGTGGCCGCGATGGCTGACACCCACCTTTTGATCACGAAACAGTCGAAAGACGGGAGAACAACAACAAGCGTCA
CACCTTTGTCAAAACAGGAAAAAATAGCGGAAATCGGCCGGATGATTGCCGGCGTTGAAGTAACGGATTTGACGAAGCGC
CATGCGAAAGAACTTTTAAATCAAGCTAAGCTAGTCAAAACCAGCGGATAA

Upstream 100 bases:

>100_bases
ATTTTGATCATCTGCCGGACGCATGATGATACGAAAGTCGTACAGAAGAAAATATTGGAGCTTTTATAAACGAAGGCCTG
AAAAGAGGTGTCATGAAAGC

Downstream 100 bases:

>100_bases
GCTGTTCATTTTTTGAACAGCTTATTTTTTTGTCGGCATTTTCTTATACAAACTCTTTCTTATCATTTTATCCAATGTTT
TTCTTCCGTTCACAGTTATA

Product: hypothetical protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MLAELSIKNFAIIEELTVSFEKGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEYVRYGEKRAELEGLFLLDGGHPVFDLC
TELGIEASDDMIVMRRDINANGKSICRVNGKLVTIAALREVGRLLLDIHGQHDNQLLMEDEHHLQLLDRFAGEEIENALK
AYQEVYSRYMDVMKKVKELSESEQEMAHRLDLIQFQLDEIESANLEPKEDELLQEERRQIANFEKIYEALQNAYNALRNE
QAGLDWVGMASSELENVSGINEGLNKVSESVANAYYILEDSTFQIRNMLDELEYDPERLDYIESRLNEIKQLKRKYGATV
EDIQAYSAKIEEEIDRIENRDSHLQALKSRLESIGKDVAIEAANVSEIRKKWAEKLAKQIQQELKDLYMEKSTFDTEFHV
KKAERDDDAPVVNGMPVQLTKHGIDTVRFLISTNTGEPLKSLSKVASGGELSRIMLAMKSIFSAQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHRVSIGSQVLCITHLPQVAAMADTHLLITKQSKDGRTTTSVTPLSKQEKIAEIGRMIAGVEVTDLTKR
HAKELLNQAKLVKTSG

Sequences:

>Translated_576_residues
MLAELSIKNFAIIEELTVSFEKGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEYVRYGEKRAELEGLFLLDGGHPVFDLC
TELGIEASDDMIVMRRDINANGKSICRVNGKLVTIAALREVGRLLLDIHGQHDNQLLMEDEHHLQLLDRFAGEEIENALK
AYQEVYSRYMDVMKKVKELSESEQEMAHRLDLIQFQLDEIESANLEPKEDELLQEERRQIANFEKIYEALQNAYNALRNE
QAGLDWVGMASSELENVSGINEGLNKVSESVANAYYILEDSTFQIRNMLDELEYDPERLDYIESRLNEIKQLKRKYGATV
EDIQAYSAKIEEEIDRIENRDSHLQALKSRLESIGKDVAIEAANVSEIRKKWAEKLAKQIQQELKDLYMEKSTFDTEFHV
KKAERDDDAPVVNGMPVQLTKHGIDTVRFLISTNTGEPLKSLSKVASGGELSRIMLAMKSIFSAQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHRVSIGSQVLCITHLPQVAAMADTHLLITKQSKDGRTTTSVTPLSKQEKIAEIGRMIAGVEVTDLTKR
HAKELLNQAKLVKTSG
>Mature_576_residues
MLAELSIKNFAIIEELTVSFEKGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEYVRYGEKRAELEGLFLLDGGHPVFDLC
TELGIEASDDMIVMRRDINANGKSICRVNGKLVTIAALREVGRLLLDIHGQHDNQLLMEDEHHLQLLDRFAGEEIENALK
AYQEVYSRYMDVMKKVKELSESEQEMAHRLDLIQFQLDEIESANLEPKEDELLQEERRQIANFEKIYEALQNAYNALRNE
QAGLDWVGMASSELENVSGINEGLNKVSESVANAYYILEDSTFQIRNMLDELEYDPERLDYIESRLNEIKQLKRKYGATV
EDIQAYSAKIEEEIDRIENRDSHLQALKSRLESIGKDVAIEAANVSEIRKKWAEKLAKQIQQELKDLYMEKSTFDTEFHV
KKAERDDDAPVVNGMPVQLTKHGIDTVRFLISTNTGEPLKSLSKVASGGELSRIMLAMKSIFSAQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHRVSIGSQVLCITHLPQVAAMADTHLLITKQSKDGRTTTSVTPLSKQEKIAEIGRMIAGVEVTDLTKR
HAKELLNQAKLVKTSG

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=571, Percent_Identity=33.9754816112084, Blast_Score=308, Evalue=7e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 64525; Mature: 64525

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAELSIKNFAIIEELTVSFEKGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEYVRYGEK
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHCHH
RAELEGLFLLDGGHPVFDLCTELGIEASDDMIVMRRDINANGKSICRVNGKLVTIAALRE
HHCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCEEHHHHHHH
VGRLLLDIHGQHDNQLLMEDEHHLQLLDRFAGEEIENALKAYQEVYSRYMDVMKKVKELS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESEQEMAHRLDLIQFQLDEIESANLEPKEDELLQEERRQIANFEKIYEALQNAYNALRNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QAGLDWVGMASSELENVSGINEGLNKVSESVANAYYILEDSTFQIRNMLDELEYDPERLD
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCHHHHH
YIESRLNEIKQLKRKYGATVEDIQAYSAKIEEEIDRIENRDSHLQALKSRLESIGKDVAI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
EAANVSEIRKKWAEKLAKQIQQELKDLYMEKSTFDTEFHVKKAERDDDAPVVNGMPVQLT
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCCCHHHH
KHGIDTVRFLISTNTGEPLKSLSKVASGGELSRIMLAMKSIFSAQQDVTSIIFDEVDTGV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGRVAQAIAEKIHRVSIGSQVLCITHLPQVAAMADTHLLITKQSKDGRTTTSVTPLSKQE
CHHHHHHHHHHHHHHHCCCCEEEEHHCHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHH
KIAEIGRMIAGVEVTDLTKRHAKELLNQAKLVKTSG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAELSIKNFAIIEELTVSFEKGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEYVRYGEK
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHCHH
RAELEGLFLLDGGHPVFDLCTELGIEASDDMIVMRRDINANGKSICRVNGKLVTIAALRE
HHCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCEEHHHHHHH
VGRLLLDIHGQHDNQLLMEDEHHLQLLDRFAGEEIENALKAYQEVYSRYMDVMKKVKELS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESEQEMAHRLDLIQFQLDEIESANLEPKEDELLQEERRQIANFEKIYEALQNAYNALRNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QAGLDWVGMASSELENVSGINEGLNKVSESVANAYYILEDSTFQIRNMLDELEYDPERLD
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCHHHHH
YIESRLNEIKQLKRKYGATVEDIQAYSAKIEEEIDRIENRDSHLQALKSRLESIGKDVAI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
EAANVSEIRKKWAEKLAKQIQQELKDLYMEKSTFDTEFHVKKAERDDDAPVVNGMPVQLT
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCCCHHHH
KHGIDTVRFLISTNTGEPLKSLSKVASGGELSRIMLAMKSIFSAQQDVTSIIFDEVDTGV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGRVAQAIAEKIHRVSIGSQVLCITHLPQVAAMADTHLLITKQSKDGRTTTSVTPLSKQE
CHHHHHHHHHHHHHHHCCCCEEEEHHCHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHH
KIAEIGRMIAGVEVTDLTKRHAKELLNQAKLVKTSG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]