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The map label for this gene is spoIVB [H]

Identifier: 52786332

GI number: 52786332

Start: 2512974

End: 2514257

Strand: Reverse

Name: spoIVB [H]

Synonym: BLi02594

Alternate gene names: 52786332

Gene position: 2514257-2512974 (Counterclockwise)

Preceding gene: 52786333

Following gene: 52786331

Centisome position: 59.54

GC content: 43.46

Gene sequence:

>1284_bases
GTGGATGTTGAAAAAATCAGAAAAGCTGTGGGTATAATTCTCCTTGTTTCTTTAATAAGTGTAGGATTTTACAAACCGGT
GAAAGAATACATTGTAATACCAAAAAATTTGAACATTTTTGAGACGGGTACCCGTGCGGTTGAAACGAGCTTATCCGTCA
GTACTCCGAAAGATGGATCATCAGAAGCATTCACATTGAAAGAAAATCAAAACGAAGTGGAAATAAAAGGCGAAAAGAAT
GGAAAGGCAGAACTCGTCTATGACTTTGCCGGATTTCCGGTTAAAAAAACGAAAGTAAATGTCCTGCCTGATTTGAAAGT
GGTTCCCGGCGGGCAGTCCATTGGTGTAAAACTTCATTCCGTTGGTGTATTGGTCGTGGGTTTTCATCAAATCAATACCT
CTGAAGGCAAAAAATCGCCCGGTGAAGCAGCCGGAATCGCGGTAGGCGACATTATCACCGAAATCAACGGCACGAAAATC
GAGAAAATGAATGACATTACCCCTTTTATTCAAAAGGCCGGAAAAACAGGAGAAACCTTACATCTGTCGATAAAAAGAGA
TAAACAGGAAATCAAAACGAAGCTCGTTCCGAAAAAAGACCAGGCTGAAGACAAATACAGAATCGGCTTATATATCAGGG
ACTCTGCCGCAGGAATCGGCACAATGACTTTTTTCGAGCCGAAATCGAAAAAATACGGAGCGCTTGGCCACGTGATTTCA
GATATGGATACGAAAAAACCGATCGTTGTTGAAAAAGGACAAATCGTGAAATCGACCGTCACCTCGATTGAAAAAGGAAC
AGGCGGAAATCCCGGAGAGAAACTGGCTCGTTTCGCATCAGAACGAAAAGTGATCGGCGATATCAACAGAAACAGTCCAT
TCGGGATCTTCGGAACGCTTCACGAGTCAATTAAAAACCGCATCGCTGACAAAGCCATTCCGATTGCATTTTCAGATGAA
GTAAAAGAAGGCCCTGCCCAAATTTTAACGGTTATTGAAAACGACAAAGTCGAGAAATTCGATATTCAGGTCGTCAGTAC
GACACCTCAGAAGTTTCCGGCCACAAAGGGCATGGTGCTGAAAATCACCGACCCGAGGCTGTTGAGTAAAACAGGAGGCA
TCGTTCAAGGGATGAGCGGAAGCCCGATCATCCAAAACGGAAAGATCATTGGCGCCGTAACCCACGTTTTCGTCAATGAT
CCGACGAGCGGGTACGGTGTGCATATTGAATGGATGCTGTCTGAAGCGGGAATCGATATTTATAAAAAAGAAAAAGCAAG
CTGA

Upstream 100 bases:

>100_bases
CTTCCGTTCACAGTTATAAATCGCCGTCCGGAAGGCAAAAGTAATGAATGTAGCGAAAGAAATTTATATGGTGTGGGTTA
GGTGCGAGGAGAGTGAAAAG

Downstream 100 bases:

>100_bases
CTGCCGGATTCCTCCGGCAGTTTTTGATTTCGCCTTTTTTTCTTGGGCGTTGTGCGTTAAAATGAAAGAAGAAAAGATTT
TTCGACAAATTCACGTTCCC

Product: SpoIVB

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDVEKIRKAVGIILLVSLISVGFYKPVKEYIVIPKNLNIFETGTRAVETSLSVSTPKDGSSEAFTLKENQNEVEIKGEKN
GKAELVYDFAGFPVKKTKVNVLPDLKVVPGGQSIGVKLHSVGVLVVGFHQINTSEGKKSPGEAAGIAVGDIITEINGTKI
EKMNDITPFIQKAGKTGETLHLSIKRDKQEIKTKLVPKKDQAEDKYRIGLYIRDSAAGIGTMTFFEPKSKKYGALGHVIS
DMDTKKPIVVEKGQIVKSTVTSIEKGTGGNPGEKLARFASERKVIGDINRNSPFGIFGTLHESIKNRIADKAIPIAFSDE
VKEGPAQILTVIENDKVEKFDIQVVSTTPQKFPATKGMVLKITDPRLLSKTGGIVQGMSGSPIIQNGKIIGAVTHVFVND
PTSGYGVHIEWMLSEAGIDIYKKEKAS

Sequences:

>Translated_427_residues
MDVEKIRKAVGIILLVSLISVGFYKPVKEYIVIPKNLNIFETGTRAVETSLSVSTPKDGSSEAFTLKENQNEVEIKGEKN
GKAELVYDFAGFPVKKTKVNVLPDLKVVPGGQSIGVKLHSVGVLVVGFHQINTSEGKKSPGEAAGIAVGDIITEINGTKI
EKMNDITPFIQKAGKTGETLHLSIKRDKQEIKTKLVPKKDQAEDKYRIGLYIRDSAAGIGTMTFFEPKSKKYGALGHVIS
DMDTKKPIVVEKGQIVKSTVTSIEKGTGGNPGEKLARFASERKVIGDINRNSPFGIFGTLHESIKNRIADKAIPIAFSDE
VKEGPAQILTVIENDKVEKFDIQVVSTTPQKFPATKGMVLKITDPRLLSKTGGIVQGMSGSPIIQNGKIIGAVTHVFVND
PTSGYGVHIEWMLSEAGIDIYKKEKAS
>Mature_427_residues
MDVEKIRKAVGIILLVSLISVGFYKPVKEYIVIPKNLNIFETGTRAVETSLSVSTPKDGSSEAFTLKENQNEVEIKGEKN
GKAELVYDFAGFPVKKTKVNVLPDLKVVPGGQSIGVKLHSVGVLVVGFHQINTSEGKKSPGEAAGIAVGDIITEINGTKI
EKMNDITPFIQKAGKTGETLHLSIKRDKQEIKTKLVPKKDQAEDKYRIGLYIRDSAAGIGTMTFFEPKSKKYGALGHVIS
DMDTKKPIVVEKGQIVKSTVTSIEKGTGGNPGEKLARFASERKVIGDINRNSPFGIFGTLHESIKNRIADKAIPIAFSDE
VKEGPAQILTVIENDKVEKFDIQVVSTTPQKFPATKGMVLKITDPRLLSKTGGIVQGMSGSPIIQNGKIIGAVTHVFVND
PTSGYGVHIEWMLSEAGIDIYKKEKAS

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 46241; Mature: 46241

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVEKIRKAVGIILLVSLISVGFYKPVKEYIVIPKNLNIFETGTRAVETSLSVSTPKDGS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCEEECCCHHEEEEEEECCCCCCC
SEAFTLKENQNEVEIKGEKNGKAELVYDFAGFPVKKTKVNVLPDLKVVPGGQSIGVKLHS
CCEEEEECCCCEEEEEECCCCCEEEEEEECCCCEECEEEEECCCCEEECCCCEEEEEEEE
VGVLVVGFHQINTSEGKKSPGEAAGIAVGDIITEINGTKIEKMNDITPFIQKAGKTGETL
EEEEEEEEEEECCCCCCCCCCCCCCEEHHHHHHHCCCCEEEHHHCCCHHHHHCCCCCCEE
HLSIKRDKQEIKTKLVPKKDQAEDKYRIGLYIRDSAAGIGTMTFFEPKSKKYGALGHVIS
EEEEECCHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHH
DMDTKKPIVVEKGQIVKSTVTSIEKGTGGNPGEKLARFASERKVIGDINRNSPFGIFGTL
CCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEHHHH
HESIKNRIADKAIPIAFSDEVKEGPAQILTVIENDKVEKFDIQVVSTTPQKFPATKGMVL
HHHHHHHHHHHCCCEEECCHHCCCHHHEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEE
KITDPRLLSKTGGIVQGMSGSPIIQNGKIIGAVTHVFVNDPTSGYGVHIEWMLSEAGIDI
EECCCHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEECCCCCE
YKKEKAS
EECCCCC
>Mature Secondary Structure
MDVEKIRKAVGIILLVSLISVGFYKPVKEYIVIPKNLNIFETGTRAVETSLSVSTPKDGS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCEEECCCHHEEEEEEECCCCCCC
SEAFTLKENQNEVEIKGEKNGKAELVYDFAGFPVKKTKVNVLPDLKVVPGGQSIGVKLHS
CCEEEEECCCCEEEEEECCCCCEEEEEEECCCCEECEEEEECCCCEEECCCCEEEEEEEE
VGVLVVGFHQINTSEGKKSPGEAAGIAVGDIITEINGTKIEKMNDITPFIQKAGKTGETL
EEEEEEEEEEECCCCCCCCCCCCCCEEHHHHHHHCCCCEEEHHHCCCHHHHHCCCCCCEE
HLSIKRDKQEIKTKLVPKKDQAEDKYRIGLYIRDSAAGIGTMTFFEPKSKKYGALGHVIS
EEEEECCHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHH
DMDTKKPIVVEKGQIVKSTVTSIEKGTGGNPGEKLARFASERKVIGDINRNSPFGIFGTL
CCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEHHHH
HESIKNRIADKAIPIAFSDEVKEGPAQILTVIENDKVEKFDIQVVSTTPQKFPATKGMVL
HHHHHHHHHHHCCCEEECCHHCCCHHHEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEE
KITDPRLLSKTGGIVQGMSGSPIIQNGKIIGAVTHVFVNDPTSGYGVHIEWMLSEAGIDI
EECCCHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEECCCCCE
YKKEKAS
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]