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The map label for this gene is spo0A [H]

Identifier: 52786331

GI number: 52786331

Start: 2511884

End: 2512684

Strand: Reverse

Name: spo0A [H]

Synonym: BLi02593

Alternate gene names: 52786331

Gene position: 2512684-2511884 (Counterclockwise)

Preceding gene: 52786332

Following gene: 52786330

Centisome position: 59.5

GC content: 48.94

Gene sequence:

>801_bases
GTGGAGAAAATTAAAGTATGTGTTGCTGATGATAACAGGGAGCTTGTCGGCCTATTAAGCGAATATATCGAGGGACAGAG
CGACATGGAAGTGCTTGGTGTCGCATACAACGGACAGGAATGCCTTTCTTTGTTTAAAGACAAGGAGCCCGACGTGCTTG
TGCTGGATATAATCATGCCTCATCTCGACGGATTGGCCGTTCTTGAAAGGCTGAGGGAATCGCAGCTTAAAAAGCAGCCG
AATGTCATCATGCTGACGGCGTTCGGCCAGGAAGACGTGACCAAAAAAGCTGTAGATTTAGGCGCGTCGTATTTTATTTT
GAAGCCTTTTGACATGGAGAATTTAGTCGGACATATCCGCCAGGTCAGCGGAAACGGATCTCAATTGACACACCGTGCCC
CGTCCTCTCAAAGCAGTGTGCTTCGGCCGCAGCCTGAATCGCCGAAGAAAAACCTGGACGCCAGCATCACGACCATCATC
CACGAAATCGGCGTACCTGCTCATATTAAAGGCTATTTGTACTTAAGAGAAGCCATCTCCATGGTATATAACGACATCGA
ACTGCTCGGCAGCATTACAAAAGTGCTTTATCCGGATATCGCCAAAAAATTCAACACGACTGCAAGCCGCGTCGAACGGG
CGATTCGCCACGCCATCGAAGTCGCATGGAGCAGAGGCAACATCGACTCGATTTCGTCACTTTTCGGCTACACAGTCAGC
ATGTCAAAAGCAAAGCCGACCAATAGTGAGTTCATCGCGATGGTCGCAGACAAGCTGAGACTGGAGCATAAGGCTTCTTA
A

Upstream 100 bases:

>100_bases
ATCCTTTTTAAAAAAAGGGAATAAAGTGGTGCTGTCGAATTAAACATATTAGAAAAAGAATGATGAAATTTATCAACATA
CACATTGGGGAGGAAGAAAC

Downstream 100 bases:

>100_bases
ACGTTGATATGACGCAGTTTTAACGAAGATTTAACCGTCGAATTTTCGACCTGTTTCTAACGAATAGTTTATCGTAATTC
CGATGAATTTTTCGCCCATA

Product: Spo0A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: Stage 0 sporulation protein C; Stage 0 sporulation protein G [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEKIKVCVADDNRELVGLLSEYIEGQSDMEVLGVAYNGQECLSLFKDKEPDVLVLDIIMPHLDGLAVLERLRESQLKKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNGSQLTHRAPSSQSSVLRPQPESPKKNLDASITTII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MSKAKPTNSEFIAMVADKLRLEHKAS

Sequences:

>Translated_266_residues
MEKIKVCVADDNRELVGLLSEYIEGQSDMEVLGVAYNGQECLSLFKDKEPDVLVLDIIMPHLDGLAVLERLRESQLKKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNGSQLTHRAPSSQSSVLRPQPESPKKNLDASITTII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MSKAKPTNSEFIAMVADKLRLEHKAS
>Mature_266_residues
MEKIKVCVADDNRELVGLLSEYIEGQSDMEVLGVAYNGQECLSLFKDKEPDVLVLDIIMPHLDGLAVLERLRESQLKKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNGSQLTHRAPSSQSSVLRPQPESPKKNLDASITTII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MSKAKPTNSEFIAMVADKLRLEHKAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=111, Percent_Identity=40.5405405405405, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789809, Length=140, Percent_Identity=34.2857142857143, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=82, Percent_Identity=39.0243902439024, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29541; Mature: 29541

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVCVADDNRELVGLLSEYIEGQSDMEVLGVAYNGQECLSLFKDKEPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEEHHH
HLDGLAVLERLRESQLKKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGNGSQLTHRAPSSQSSVLRPQPESPKKNLDASITTIIHEIGVPAHIKGYLYLREAIS
HHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEE
MSKAKPTNSEFIAMVADKLRLEHKAS
CCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKIKVCVADDNRELVGLLSEYIEGQSDMEVLGVAYNGQECLSLFKDKEPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEEHHH
HLDGLAVLERLRESQLKKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGNGSQLTHRAPSSQSSVLRPQPESPKKNLDASITTIIHEIGVPAHIKGYLYLREAIS
HHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEE
MSKAKPTNSEFIAMVADKLRLEHKAS
CCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3921963; 3018427; 3157992; 8969508; 9384377; 9044256; 3151993; 2121995; 12176382 [H]