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The map label for this gene is ribE [H]

Identifier: 52786213

GI number: 52786213

Start: 2407262

End: 2407909

Strand: Reverse

Name: ribE [H]

Synonym: BLi02474

Alternate gene names: 52786213

Gene position: 2407909-2407262 (Counterclockwise)

Preceding gene: 52786214

Following gene: 52786212

Centisome position: 57.02

GC content: 47.69

Gene sequence:

>648_bases
ATGTTTACAGGCATCATTGAAGAAGTCGGAACGATTTTGGACATGAAAAAGGCGGGAAGCGCCATGTCTTTAGTCATTCA
ATCCAACAAAGTGATCGAAGATGTGAAGCTCGGCGACAGCATTGCTGTCAACGGAGTCTGCCTGACCGTAAATGAGTTCG
GTTCCAGCAGTTTCACGGCGGATGTCATGCTTGAAACGCTGAAAGCAACATCACTCGGCACATTGAAAAAAGGAAGCCGC
GTCAATCTTGAACGGGCGATGGCGGCAAACGGCCGCTTCGGCGGGCATATGGTGTCGGGGCATGTCGACGGCACTGCATC
GATCGTTCGCATAGAAAAAGCGGCAAACACCGTATACTATGATTTAAAACTTGACCCTTCACTGGCCAAAATGCTTGTGT
TAAAAGGTTCGATCGCCGTAGACGGCGTCAGCCTCACCATATTCGGACTGACGGAGGATCAGGTAACAGTCTCGCTTATC
CCGCACACATTGGATGAAACGATTTTTCCTGCAAAAAAGGTCGGAAGCATCGTCAACATCGAGTGCGATATGATCGGCAA
ATACATTTACCGCTTTCTCCACCAAACAAAACAAGAAAAACAGGCACAGGCATTGACTGAAGCATTCTTTAAAGAAAACG
GGTTTTAA

Upstream 100 bases:

>100_bases
CGATGAAAGATGTCCCCTTACTGCAATTCACAACCATTACCAAGATCGGCCAGGATATAAAGCTGGCAGCAAGACCGATA
AAAGAATAAGGCGGTGACAT

Downstream 100 bases:

>100_bases
ATGAAAGGAGGCTGTGCGTCTATGTTTCACACAATTGAAGAAGCGATTCGCGATTTGAAGCAGGGAAAAGTCATCATCGT
AGTCGATGACGAGAACAGGG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MFTGIIEEVGTILDMKKAGSAMSLVIQSNKVIEDVKLGDSIAVNGVCLTVNEFGSSSFTADVMLETLKATSLGTLKKGSR
VNLERAMAANGRFGGHMVSGHVDGTASIVRIEKAANTVYYDLKLDPSLAKMLVLKGSIAVDGVSLTIFGLTEDQVTVSLI
PHTLDETIFPAKKVGSIVNIECDMIGKYIYRFLHQTKQEKQAQALTEAFFKENGF

Sequences:

>Translated_215_residues
MFTGIIEEVGTILDMKKAGSAMSLVIQSNKVIEDVKLGDSIAVNGVCLTVNEFGSSSFTADVMLETLKATSLGTLKKGSR
VNLERAMAANGRFGGHMVSGHVDGTASIVRIEKAANTVYYDLKLDPSLAKMLVLKGSIAVDGVSLTIFGLTEDQVTVSLI
PHTLDETIFPAKKVGSIVNIECDMIGKYIYRFLHQTKQEKQAQALTEAFFKENGF
>Mature_215_residues
MFTGIIEEVGTILDMKKAGSAMSLVIQSNKVIEDVKLGDSIAVNGVCLTVNEFGSSSFTADVMLETLKATSLGTLKKGSR
VNLERAMAANGRFGGHMVSGHVDGTASIVRIEKAANTVYYDLKLDPSLAKMLVLKGSIAVDGVSLTIFGLTEDQVTVSLI
PHTLDETIFPAKKVGSIVNIECDMIGKYIYRFLHQTKQEKQAQALTEAFFKENGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=186, Percent_Identity=37.0967741935484, Blast_Score=124, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319733, Length=205, Percent_Identity=42.4390243902439, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23184; Mature: 23184

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIEEVGTILDMKKAGSAMSLVIQSNKVIEDVKLGDSIAVNGVCLTVNEFGSSSFTA
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCEEECCEEEEEECCCCCCCHH
DVMLETLKATSLGTLKKGSRVNLERAMAANGRFGGHMVSGHVDGTASIVRIEKAANTVYY
HHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCEEEE
DLKLDPSLAKMLVLKGSIAVDGVSLTIFGLTEDQVTVSLIPHTLDETIFPAKKVGSIVNI
EEEECHHHHHHHHHCCCEEECCEEEEEEEECCCCEEEEECCCHHHHHCCCHHHCCCEEEE
ECDMIGKYIYRFLHQTKQEKQAQALTEAFFKENGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIIEEVGTILDMKKAGSAMSLVIQSNKVIEDVKLGDSIAVNGVCLTVNEFGSSSFTA
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCEEECCEEEEEECCCCCCCHH
DVMLETLKATSLGTLKKGSRVNLERAMAANGRFGGHMVSGHVDGTASIVRIEKAANTVYY
HHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCEEEE
DLKLDPSLAKMLVLKGSIAVDGVSLTIFGLTEDQVTVSLIPHTLDETIFPAKKVGSIVNI
EEEECHHHHHHHHHCCCEEECCEEEEEEEECCCCEEEEECCCHHHHHCCCHHHCCCEEEE
ECDMIGKYIYRFLHQTKQEKQAQALTEAFFKENGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2106516; 7934829; 9384377; 7473709 [H]