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The map label for this gene is ubiE

Identifier: 52786154

GI number: 52786154

Start: 2353545

End: 2354243

Strand: Reverse

Name: ubiE

Synonym: BLi02410

Alternate gene names: 52786154

Gene position: 2354243-2353545 (Counterclockwise)

Preceding gene: 52786155

Following gene: 52786153

Centisome position: 55.75

GC content: 48.78

Gene sequence:

>699_bases
ATGGTGCAGTCAAAAGAACAGCGCGTACATGGCGTGTTTGAAAAGATTTATAAAAATTATGACCAGATGAACTCCGTCAT
CAGCTTTAAACAGCATAAAAAATGGCGCGACAAAACGATGCAGCTCATGAACGTTCCAAAAGGAGCCACAGCGCTTGACG
TCTGCTGCGGGACGGGAGACTGGACGATCGCGCTCGCTGATGCGGCAGGAGAGACAGGGGAAATCAAGGGCCTTGATTTC
AGCAAAAACATGCTGAGCATCGCCGAACAAAAAACGGAAAGCTACAGCCAGATCGAATTGATCCACGGCAATGCGATGGA
ACTGCCCTTCCCGGATAATACATTCGACTATGTCACCATCGGCTTCGGGCTTCGCAATGTTCCCGACTATTTAACCGTAT
TAAAAGAGATGGCGCGCGTGGTAAAACCCGGCGGCCAGGTTGTATGCCTTGAGACGTCCCAGCCTGAAATGTTCGGGTTC
AAACAGCTATACTTCCTGTACTTCCGCTTCATTATGCCTATGTTTGGAAAGCTCTTTGCCAAAAGCTTCAAGGAGTATTC
ATGGCTTCAGGAATCGGCCAGGGAATTTCCTGGTATGAAAGAGCTTGCCGCGTTGTTTGAAGAGGCTGGATTAAAAAATG
TTCAATATCATCCGTTCACAGGCGGCGTAGCGGCAACACATGTCGGCTGGAAGCGATGA

Upstream 100 bases:

>100_bases
TTGCCAAAAAGCCCGCCGTCCAAAAATGGCTTCTGAAACGCCTGCAGGAAATTCGGCCTGAAAAACGAACTTATCATCAG
AAAGTGGAAGAAGGGTAATC

Downstream 100 bases:

>100_bases
TGACTACAGAAATACTCGGGTGAATCGATATGAAATTGACAACGTTTTATTCTTTTTTGAATGACGATATTGAATTAATA
GAAAAAGAGCTTGAAAAGAC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MVQSKEQRVHGVFEKIYKNYDQMNSVISFKQHKKWRDKTMQLMNVPKGATALDVCCGTGDWTIALADAAGETGEIKGLDF
SKNMLSIAEQKTESYSQIELIHGNAMELPFPDNTFDYVTIGFGLRNVPDYLTVLKEMARVVKPGGQVVCLETSQPEMFGF
KQLYFLYFRFIMPMFGKLFAKSFKEYSWLQESAREFPGMKELAALFEEAGLKNVQYHPFTGGVAATHVGWKR

Sequences:

>Translated_232_residues
MVQSKEQRVHGVFEKIYKNYDQMNSVISFKQHKKWRDKTMQLMNVPKGATALDVCCGTGDWTIALADAAGETGEIKGLDF
SKNMLSIAEQKTESYSQIELIHGNAMELPFPDNTFDYVTIGFGLRNVPDYLTVLKEMARVVKPGGQVVCLETSQPEMFGF
KQLYFLYFRFIMPMFGKLFAKSFKEYSWLQESAREFPGMKELAALFEEAGLKNVQYHPFTGGVAATHVGWKR
>Mature_232_residues
MVQSKEQRVHGVFEKIYKNYDQMNSVISFKQHKKWRDKTMQLMNVPKGATALDVCCGTGDWTIALADAAGETGEIKGLDF
SKNMLSIAEQKTESYSQIELIHGNAMELPFPDNTFDYVTIGFGLRNVPDYLTVLKEMARVVKPGGQVVCLETSQPEMFGF
KQLYFLYFRFIMPMFGKLFAKSFKEYSWLQESAREFPGMKELAALFEEAGLKNVQYHPFTGGVAATHVGWKR

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=265, Percent_Identity=33.5849056603774, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI48994983, Length=234, Percent_Identity=38.8888888888889, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17557039, Length=232, Percent_Identity=35.3448275862069, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323526, Length=250, Percent_Identity=29.6, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641702, Length=243, Percent_Identity=32.5102880658436, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_BACLD (Q65I24)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079575.1
- RefSeq:   YP_091983.1
- ProteinModelPortal:   Q65I24
- SMR:   Q65I24
- STRING:   Q65I24
- EnsemblBacteria:   EBBACT00000056855
- EnsemblBacteria:   EBBACT00000061412
- GeneID:   3027342
- GeneID:   3101295
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02410
- KEGG:   bli:BL02783
- NMPDR:   fig|279010.5.peg.261
- eggNOG:   COG2226
- GeneTree:   EBGT00050000000522
- HOGENOM:   HBG559580
- OMA:   GGAISKN
- ProtClustDB:   PRK00216
- BioCyc:   BLIC279010-1:BLI02410-MONOMER
- BioCyc:   BLIC279010:BL02783-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR014122
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR02752
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 26418; Mature: 26418

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSKEQRVHGVFEKIYKNYDQMNSVISFKQHKKWRDKTMQLMNVPKGATALDVCCGTGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCC
WTIALADAAGETGEIKGLDFSKNMLSIAEQKTESYSQIELIHGNAMELPFPDNTFDYVTI
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHEEEEEECCEEECCCCCCCCCEEEE
GFGLRNVPDYLTVLKEMARVVKPGGQVVCLETSQPEMFGFKQLYFLYFRFIMPMFGKLFA
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSFKEYSWLQESAREFPGMKELAALFEEAGLKNVQYHPFTGGVAATHVGWKR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCCCHHHCCCCCCC
>Mature Secondary Structure
MVQSKEQRVHGVFEKIYKNYDQMNSVISFKQHKKWRDKTMQLMNVPKGATALDVCCGTGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCC
WTIALADAAGETGEIKGLDFSKNMLSIAEQKTESYSQIELIHGNAMELPFPDNTFDYVTI
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHEEEEEECCEEECCCCCCCCCEEEE
GFGLRNVPDYLTVLKEMARVVKPGGQVVCLETSQPEMFGFKQLYFLYFRFIMPMFGKLFA
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSFKEYSWLQESAREFPGMKELAALFEEAGLKNVQYHPFTGGVAATHVGWKR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA