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The map label for this gene is cca

Identifier: 52786124

GI number: 52786124

Start: 2326496

End: 2327689

Strand: Reverse

Name: cca

Synonym: BLi02380

Alternate gene names: 52786124

Gene position: 2327689-2326496 (Counterclockwise)

Preceding gene: 52786125

Following gene: 52786123

Centisome position: 55.12

GC content: 51.09

Gene sequence:

>1194_bases
ATGGAGGATCTCTTTTTGAAGGCTCTTCCTCTCCTTCGTGAGCTGAAAAAACACGGCTGGCAGGCCTACTTTGTAGGCGG
AGCCGTCAGGGATGCCCAAATGAACAGAGATGTCGGCGATATTGATATCGCTACAGATGCATCCCCGGAAGAAATCGAAG
CCATTTTTCCGAAAACCGTTGATGTCGGAAAGGAACACGGAACCATCATCGTCCTCTTTGAGGGAGAATCCTACGAGGTG
ACGACCTTTCGCGCAGAGCTTGAATATGAGGATTACAGAAGGCCGAGCGGCGTTCAGTTTATCAAATCGCTGAAGGAAGA
CCTGAAGAGGCGGGATCTGACGATTAATGCGATGGCGATGGATGAAGAAGGCAGACTGATCGACTATTTCGGCGGGCTCC
GCGACATACGGGAAAGATTGATTCAAACGGTCGGCGACCCGGCGGAGCGTTTTCACGAAGATGCGCTTCGCATGCTGCGG
GCGCTCCGTTTTATGAGTCAGCTCGAGTTTGAGCTTTCTCCAAATACAAAAAAAGCGATTTGCGAAAACCGTCCGCTCCT
GGCCCATATTTCTACAGAGCGGAAAACGGTTGAATTCGAAAAACTTTTAAAAGGAAAAGCAGCAGGCCGGGCTCTGGAAG
CAGCGGCTGAAACAGGGCTTTACAAGGAGCTTCCGGGGCTGGACGGCAAAAAGGAGCGCGTTCTGGCTGCCCGAGCTTTT
CCTTTTTATCAACTTCAAAAAAGTGCCGATATATGGGCGGCTTTCATTCACATGATATCGATCAATCCCGCTGAAGCGCA
CCGGTTTTTAAAAGGGTGGAAGCTTCCGGGGAAAGTCATCAGACAGGCGCTCCATACGGCTGAACGGATCGATCAAAAAT
GGGATGCCGTCTCGATGTATGAGGCGGGGGAAGAGACGCTGCTGGCCGCATCTGCCATTCGCAGCTTGAGGGACAAGCAG
GCAATCGATGAAGAGCAGCTGGCTGAAATCAGACAGTCGTACCAGGCGCTTCCGATTAAAAGCCTGAAAGACCTGGCGGT
ATCTGGAAGCGATCTGCTGAATTTCAGAAAGAAGCCGGCCGGAAAATGGGTGTCCGATGATTTGAAACGGATTGAGCGGG
CCGTGTTAAACGGGGAGCTTGCCAATCAAAAAAAAGCCATAGAGGAGTGGCTGGATTCATGCAGTCAAATTTAA

Upstream 100 bases:

>100_bases
GAAGCGGCGCTGCAAAGCATCAAGGAACGCTTTTCTTCCAACAAAATCATCGCGCAATATGAAGAAATTTATCAACAATT
AACAGACTGGGAGTGTGACA

Downstream 100 bases:

>100_bases
GAAAGAAACTCATTCAATTGTTTACCGAGGCAGACGGCGAATTCATTTCTGGCCAGAAAATCAGCGAAGAGCTCGGATGC
TCAAGAACCGCCGTTTGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MEDLFLKALPLLRELKKHGWQAYFVGGAVRDAQMNRDVGDIDIATDASPEEIEAIFPKTVDVGKEHGTIIVLFEGESYEV
TTFRAELEYEDYRRPSGVQFIKSLKEDLKRRDLTINAMAMDEEGRLIDYFGGLRDIRERLIQTVGDPAERFHEDALRMLR
ALRFMSQLEFELSPNTKKAICENRPLLAHISTERKTVEFEKLLKGKAAGRALEAAAETGLYKELPGLDGKKERVLAARAF
PFYQLQKSADIWAAFIHMISINPAEAHRFLKGWKLPGKVIRQALHTAERIDQKWDAVSMYEAGEETLLAASAIRSLRDKQ
AIDEEQLAEIRQSYQALPIKSLKDLAVSGSDLLNFRKKPAGKWVSDDLKRIERAVLNGELANQKKAIEEWLDSCSQI

Sequences:

>Translated_397_residues
MEDLFLKALPLLRELKKHGWQAYFVGGAVRDAQMNRDVGDIDIATDASPEEIEAIFPKTVDVGKEHGTIIVLFEGESYEV
TTFRAELEYEDYRRPSGVQFIKSLKEDLKRRDLTINAMAMDEEGRLIDYFGGLRDIRERLIQTVGDPAERFHEDALRMLR
ALRFMSQLEFELSPNTKKAICENRPLLAHISTERKTVEFEKLLKGKAAGRALEAAAETGLYKELPGLDGKKERVLAARAF
PFYQLQKSADIWAAFIHMISINPAEAHRFLKGWKLPGKVIRQALHTAERIDQKWDAVSMYEAGEETLLAASAIRSLRDKQ
AIDEEQLAEIRQSYQALPIKSLKDLAVSGSDLLNFRKKPAGKWVSDDLKRIERAVLNGELANQKKAIEEWLDSCSQI
>Mature_397_residues
MEDLFLKALPLLRELKKHGWQAYFVGGAVRDAQMNRDVGDIDIATDASPEEIEAIFPKTVDVGKEHGTIIVLFEGESYEV
TTFRAELEYEDYRRPSGVQFIKSLKEDLKRRDLTINAMAMDEEGRLIDYFGGLRDIRERLIQTVGDPAERFHEDALRMLR
ALRFMSQLEFELSPNTKKAICENRPLLAHISTERKTVEFEKLLKGKAAGRALEAAAETGLYKELPGLDGKKERVLAARAF
PFYQLQKSADIWAAFIHMISINPAEAHRFLKGWKLPGKVIRQALHTAERIDQKWDAVSMYEAGEETLLAASAIRSLRDKQ
AIDEEQLAEIRQSYQALPIKSLKDLAVSGSDLLNFRKKPAGKWVSDDLKRIERAVLNGELANQKKAIEEWLDSCSQI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=222, Percent_Identity=35.1351351351351, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI87081691, Length=234, Percent_Identity=32.4786324786325, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789436, Length=231, Percent_Identity=32.034632034632, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=233, Percent_Identity=36.9098712446352, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI281360112, Length=201, Percent_Identity=36.81592039801, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357337, Length=201, Percent_Identity=36.81592039801, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BACLD (Q65I54)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079544.1
- RefSeq:   YP_091953.1
- ProteinModelPortal:   Q65I54
- STRING:   Q65I54
- EnsemblBacteria:   EBBACT00000055245
- EnsemblBacteria:   EBBACT00000058755
- GeneID:   3028531
- GeneID:   3100180
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02380
- KEGG:   bli:BL02754
- NMPDR:   fig|279010.5.peg.230
- eggNOG:   COG0617
- GeneTree:   EBGT00050000000996
- HOGENOM:   HBG635063
- OMA:   DHGNGYE
- ProtClustDB:   PRK13299
- BioCyc:   BLIC279010-1:BLI02380-MONOMER
- BioCyc:   BLIC279010:BL02754-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45117; Mature: 45117

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 30-30 BINDING 111-111 BINDING 154-154 BINDING 157-157 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDLFLKALPLLRELKKHGWQAYFVGGAVRDAQMNRDVGDIDIATDASPEEIEAIFPKTV
CCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCEEECCCCCHHHHHHHCCCHH
DVGKEHGTIIVLFEGESYEVTTFRAELEYEDYRRPSGVQFIKSLKEDLKRRDLTINAMAM
HCCCCCCEEEEEEECCCEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
DEEGRLIDYFGGLRDIRERLIQTVGDPAERFHEDALRMLRALRFMSQLEFELSPNTKKAI
CCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
CENRPLLAHISTERKTVEFEKLLKGKAAGRALEAAAETGLYKELPGLDGKKERVLAARAF
HCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCC
PFYQLQKSADIWAAFIHMISINPAEAHRFLKGWKLPGKVIRQALHTAERIDQKWDAVSMY
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
EAGEETLLAASAIRSLRDKQAIDEEQLAEIRQSYQALPIKSLKDLAVSGSDLLNFRKKPA
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCC
GKWVSDDLKRIERAVLNGELANQKKAIEEWLDSCSQI
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEDLFLKALPLLRELKKHGWQAYFVGGAVRDAQMNRDVGDIDIATDASPEEIEAIFPKTV
CCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCEEECCCCCHHHHHHHCCCHH
DVGKEHGTIIVLFEGESYEVTTFRAELEYEDYRRPSGVQFIKSLKEDLKRRDLTINAMAM
HCCCCCCEEEEEEECCCEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
DEEGRLIDYFGGLRDIRERLIQTVGDPAERFHEDALRMLRALRFMSQLEFELSPNTKKAI
CCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
CENRPLLAHISTERKTVEFEKLLKGKAAGRALEAAAETGLYKELPGLDGKKERVLAARAF
HCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCC
PFYQLQKSADIWAAFIHMISINPAEAHRFLKGWKLPGKVIRQALHTAERIDQKWDAVSMY
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
EAGEETLLAASAIRSLRDKQAIDEEQLAEIRQSYQALPIKSLKDLAVSGSDLLNFRKKPA
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCC
GKWVSDDLKRIERAVLNGELANQKKAIEEWLDSCSQI
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA