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The map label for this gene is aprX [H]

Identifier: 52785934

GI number: 52785934

Start: 2148972

End: 2150300

Strand: Direct

Name: aprX [H]

Synonym: BLi02185

Alternate gene names: 52785934

Gene position: 2148972-2150300 (Clockwise)

Preceding gene: 52785932

Following gene: 52785938

Centisome position: 50.89

GC content: 47.63

Gene sequence:

>1329_bases
ATGTTCGGTTCCTACATGGTTCAAATGGTAAGGTCCAACGCCCATAAGCTGGACCGGCCGCTGAGAGAAAGCGTGCTGAA
ACTCTATAAACCTTTTAAATGGACGCCTTGCTTTTTACACGGTTTTTTCGAAAAAATATTAATGAAAAACAAAAAAATAC
CAGTCATTATTGAATTTGAAAAAGACTGTCAGCATAAAGGCTATCAATTAGTCAATGAAATGATCGGCAGCAAAAGAAGA
AACAAAATCAAGCATCGCTTCTCCAACGTTAGCTGCTGCAGCGCTGAAGTCACCCCTTCTTCTTTGCAATCGCTTCTATC
TGAGTGCGGCGACATTCGCAAAATTTATTTAAATCGCAAAGTAAAAGCGCTCCTCGACGTAGCGGTTGAATCAAGCCATG
CCAAAGAAGTGGTCAGAAACAATCAGACGCTGACAGGCAAAGGAGTGACAGTCGCTGTCATTGATACGGGCGTATACCCT
CACGAAGATCTTGAAGGCAGGATCAGGGCTTTTCAAGACTTTATCAACCAGAGAACAGAACCCTATGATGACAATGGGCA
CGGCACACACTGCGCCGGTGATGCTTGCGGAAACGGAGCGGCCTCATCGGGTCAGTACCGCGGACCTGCTCCTGAAGCAG
AACTTGTCGGTGTAAAAGTATTGGACAAAATGGGATCCGGATCGCTCGAAACCGTCATTCAAGGCGTAGATTGGTGCATT
CAATTCAATAAGGAAAATCCTGATGATCCGATCGACATTATTTCAATGTCATTGGGTGCAGAAGCCTTGCGCTACGAGAA
TGAAGAAGAAGATCCAGTCGTTAAAGCTGTTCATGCAGCATGGGACGCAGGCATTGTTGTATGTGCGGCAGCCGGCAACT
CCGGTCCTGATGCGCAAACGATTGCCAGCCCGGGTGTCAGCAGCAAGATTATTACAGTCGGAGCCTTGGATGACAGGGAT
ACAGTCAGCCGGGAGGATGACGATGTCGCCTCTTATTCAAGCAGAGGGCCGACAATCTATGGTCAAGTCAAACCGGACTT
GCTGGTACCGGGCACAAATATTACGTCGCTTCGTTCACCCGGATCTTTTCTCGATAAGCTGCAAAAAACAAACAGAGTCG
GCACAAAATATATGACATTGTCCGGAACCTCGATGGCTACGCCGATCTGCGCAGGAATTGCCGCACTTATCCTTCAGCAA
GCCCCGGGCACAGAACCTGATGAAGTCAAACAGCTGCTAATGGACGGTACCGATTTATGGAAAGATCGCGATCCAAATGT
TTACGGTGCAGGGTACATCAACGCAGAACAATCGGTGCCGCAAGACTAA

Upstream 100 bases:

>100_bases
CTTTTTTGCCGCCGTTTTAGGACAGCCAACTTTACGGGAACCGCCTCTCATTCATAGATTAAGAGGAAGAATAAACTATA
AGAGATGAGGTGGAGCTAGA

Downstream 100 bases:

>100_bases
AGACCGTTCGCCCGTTTTTGCAATAAGCGGGCGAATCTTACATAAAAATATTTAAACTGCAGACCTTCTTTTTTCAAGGA
TGAGCGGTATCGTCAAATAC

Product: AprX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MFGSYMVQMVRSNAHKLDRPLRESVLKLYKPFKWTPCFLHGFFEKILMKNKKIPVIIEFEKDCQHKGYQLVNEMIGSKRR
NKIKHRFSNVSCCSAEVTPSSLQSLLSECGDIRKIYLNRKVKALLDVAVESSHAKEVVRNNQTLTGKGVTVAVIDTGVYP
HEDLEGRIRAFQDFINQRTEPYDDNGHGTHCAGDACGNGAASSGQYRGPAPEAELVGVKVLDKMGSGSLETVIQGVDWCI
QFNKENPDDPIDIISMSLGAEALRYENEEEDPVVKAVHAAWDAGIVVCAAAGNSGPDAQTIASPGVSSKIITVGALDDRD
TVSREDDDVASYSSRGPTIYGQVKPDLLVPGTNITSLRSPGSFLDKLQKTNRVGTKYMTLSGTSMATPICAGIAALILQQ
APGTEPDEVKQLLMDGTDLWKDRDPNVYGAGYINAEQSVPQD

Sequences:

>Translated_442_residues
MFGSYMVQMVRSNAHKLDRPLRESVLKLYKPFKWTPCFLHGFFEKILMKNKKIPVIIEFEKDCQHKGYQLVNEMIGSKRR
NKIKHRFSNVSCCSAEVTPSSLQSLLSECGDIRKIYLNRKVKALLDVAVESSHAKEVVRNNQTLTGKGVTVAVIDTGVYP
HEDLEGRIRAFQDFINQRTEPYDDNGHGTHCAGDACGNGAASSGQYRGPAPEAELVGVKVLDKMGSGSLETVIQGVDWCI
QFNKENPDDPIDIISMSLGAEALRYENEEEDPVVKAVHAAWDAGIVVCAAAGNSGPDAQTIASPGVSSKIITVGALDDRD
TVSREDDDVASYSSRGPTIYGQVKPDLLVPGTNITSLRSPGSFLDKLQKTNRVGTKYMTLSGTSMATPICAGIAALILQQ
APGTEPDEVKQLLMDGTDLWKDRDPNVYGAGYINAEQSVPQD
>Mature_442_residues
MFGSYMVQMVRSNAHKLDRPLRESVLKLYKPFKWTPCFLHGFFEKILMKNKKIPVIIEFEKDCQHKGYQLVNEMIGSKRR
NKIKHRFSNVSCCSAEVTPSSLQSLLSECGDIRKIYLNRKVKALLDVAVESSHAKEVVRNNQTLTGKGVTVAVIDTGVYP
HEDLEGRIRAFQDFINQRTEPYDDNGHGTHCAGDACGNGAASSGQYRGPAPEAELVGVKVLDKMGSGSLETVIQGVDWCI
QFNKENPDDPIDIISMSLGAEALRYENEEEDPVVKAVHAAWDAGIVVCAAAGNSGPDAQTIASPGVSSKIITVGALDDRD
TVSREDDDVASYSSRGPTIYGQVKPDLLVPGTNITSLRSPGSFLDKLQKTNRVGTKYMTLSGTSMATPICAGIAALILQQ
APGTEPDEVKQLLMDGTDLWKDRDPNVYGAGYINAEQSVPQD

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=280, Percent_Identity=27.5, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324576, Length=285, Percent_Identity=34.7368421052632, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319893, Length=270, Percent_Identity=34.0740740740741, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320775, Length=269, Percent_Identity=34.2007434944238, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45550681, Length=307, Percent_Identity=28.0130293159609, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24653270, Length=268, Percent_Identity=29.1044776119403, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 48227; Mature: 48227

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGSYMVQMVRSNAHKLDRPLRESVLKLYKPFKWTPCFLHGFFEKILMKNKKIPVIIEFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
KDCQHKGYQLVNEMIGSKRRNKIKHRFSNVSCCSAEVTPSSLQSLLSECGDIRKIYLNRK
HHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKALLDVAVESSHAKEVVRNNQTLTGKGVTVAVIDTGVYPHEDLEGRIRAFQDFINQRTE
HHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
PYDDNGHGTHCAGDACGNGAASSGQYRGPAPEAELVGVKVLDKMGSGSLETVIQGVDWCI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHEE
QFNKENPDDPIDIISMSLGAEALRYENEEEDPVVKAVHAAWDAGIVVCAAAGNSGPDAQT
EECCCCCCCHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHH
IASPGVSSKIITVGALDDRDTVSREDDDVASYSSRGPTIYGQVKPDLLVPGTNITSLRSP
HCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCC
GSFLDKLQKTNRVGTKYMTLSGTSMATPICAGIAALILQQAPGTEPDEVKQLLMDGTDLW
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHC
KDRDPNVYGAGYINAEQSVPQD
CCCCCCEEECEEECCCCCCCCC
>Mature Secondary Structure
MFGSYMVQMVRSNAHKLDRPLRESVLKLYKPFKWTPCFLHGFFEKILMKNKKIPVIIEFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
KDCQHKGYQLVNEMIGSKRRNKIKHRFSNVSCCSAEVTPSSLQSLLSECGDIRKIYLNRK
HHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKALLDVAVESSHAKEVVRNNQTLTGKGVTVAVIDTGVYPHEDLEGRIRAFQDFINQRTE
HHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
PYDDNGHGTHCAGDACGNGAASSGQYRGPAPEAELVGVKVLDKMGSGSLETVIQGVDWCI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHEE
QFNKENPDDPIDIISMSLGAEALRYENEEEDPVVKAVHAAWDAGIVVCAAAGNSGPDAQT
EECCCCCCCHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHH
IASPGVSSKIITVGALDDRDTVSREDDDVASYSSRGPTIYGQVKPDLLVPGTNITSLRSP
HCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCC
GSFLDKLQKTNRVGTKYMTLSGTSMATPICAGIAALILQQAPGTEPDEVKQLLMDGTDLW
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHC
KDRDPNVYGAGYINAEQSVPQD
CCCCCCEEECEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]