Click here to switch to the map view.

The map label for this gene is yitB [H]

Identifier: 52785904

GI number: 52785904

Start: 2102543

End: 2103253

Strand: Reverse

Name: yitB [H]

Synonym: BLi02154

Alternate gene names: 52785904

Gene position: 2103253-2102543 (Counterclockwise)

Preceding gene: 52785906

Following gene: 52785903

Centisome position: 49.81

GC content: 48.52

Gene sequence:

>711_bases
ATGAACGATGTTTTAACGTATAGCACATGGGATGAAAAAGCTTCTGAAGAGGTGATGGATACTTTCAGCGGCGAGATGGA
CGTTTTGAAATGGGCGTACAGCACGTATCAAAAAATCGTTTACGCCTGCAGCTTCGGCGCGGAAGGAATCGTTTTGATCG
ACTTGATTTCAAAGGTTGTGAAAGAAGCGGACATCGTGTTTTTAGATACAGGACTGCATTTTCCGGAAACATATGAGCTG
ATCAATGAAGTGAAAGATCGCTATCCCGGTCTGTCGATCCACTTTTTAAAACCCGGCCTCTCACTTGAAGAGCAGGAAAA
ACGCTATGGCAGCGAACTTTGGAAGCGCCAGCCGGACCAGTGCTGCCGCCTTCGCAAAATCGAGCCGCTCAGGGAGCATC
TGTCAGGCATGGAAGCCTGGATTACCGGGCTGAGGCGGGAGCAGTCGGAAACACGGAAACATGTCCGATATATCAACAAG
GACGACAATTTTAAACTGATTAAAATCTGCCCCCTTATTCACTGGACATGGGAGGATGTCTGGACTTACATTAAGCTGAA
TCAGCTGACGTATCACAAGCTTCATGATCAAGATTATCCGAGCATCGGCTGTGAGGTGTGTACGCTTCCCGTGACAGACA
GCGGCAATTTAAGAGCGGGAAGGTGGGCCCACCAGGAAAAAACAGAATGCGGGCTGCATCAATTCCGGTAG

Upstream 100 bases:

>100_bases
TAGTCTAAAAAGTGAATAATCTGCCTATATGTTCTTTTAGTTCATGTTTCGTATCAGAGGATAAGAAAACAAACCTTTAG
GACGTATTGGGGGAAAATCG

Downstream 100 bases:

>100_bases
GAGCTGGGTAAAAAATGACGATTACAATTTTGGCATTCATTATCGCTTTGTTTTTTGCCATGAACATAGGAGCGAGCGGA
GCAGCAGCGTCGATGGGAAT

Product: YitB

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNDVLTYSTWDEKASEEVMDTFSGEMDVLKWAYSTYQKIVYACSFGAEGIVLIDLISKVVKEADIVFLDTGLHFPETYEL
INEVKDRYPGLSIHFLKPGLSLEEQEKRYGSELWKRQPDQCCRLRKIEPLREHLSGMEAWITGLRREQSETRKHVRYINK
DDNFKLIKICPLIHWTWEDVWTYIKLNQLTYHKLHDQDYPSIGCEVCTLPVTDSGNLRAGRWAHQEKTECGLHQFR

Sequences:

>Translated_236_residues
MNDVLTYSTWDEKASEEVMDTFSGEMDVLKWAYSTYQKIVYACSFGAEGIVLIDLISKVVKEADIVFLDTGLHFPETYEL
INEVKDRYPGLSIHFLKPGLSLEEQEKRYGSELWKRQPDQCCRLRKIEPLREHLSGMEAWITGLRREQSETRKHVRYINK
DDNFKLIKICPLIHWTWEDVWTYIKLNQLTYHKLHDQDYPSIGCEVCTLPVTDSGNLRAGRWAHQEKTECGLHQFR
>Mature_236_residues
MNDVLTYSTWDEKASEEVMDTFSGEMDVLKWAYSTYQKIVYACSFGAEGIVLIDLISKVVKEADIVFLDTGLHFPETYEL
INEVKDRYPGLSIHFLKPGLSLEEQEKRYGSELWKRQPDQCCRLRKIEPLREHLSGMEAWITGLRREQSETRKHVRYINK
DDNFKLIKICPLIHWTWEDVWTYIKLNQLTYHKLHDQDYPSIGCEVCTLPVTDSGNLRAGRWAHQEKTECGLHQFR

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=200, Percent_Identity=30.5, Blast_Score=91, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325425, Length=218, Percent_Identity=34.8623853211009, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 27765; Mature: 27765

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVLTYSTWDEKASEEVMDTFSGEMDVLKWAYSTYQKIVYACSFGAEGIVLIDLISKVV
CCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KEADIVFLDTGLHFPETYELINEVKDRYPGLSIHFLKPGLSLEEQEKRYGSELWKRQPDQ
HHCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHH
CCRLRKIEPLREHLSGMEAWITGLRREQSETRKHVRYINKDDNFKLIKICPLIHWTWEDV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHEECHHHH
WTYIKLNQLTYHKLHDQDYPSIGCEVCTLPVTDSGNLRAGRWAHQEKTECGLHQFR
HHHHHHCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHCCC
>Mature Secondary Structure
MNDVLTYSTWDEKASEEVMDTFSGEMDVLKWAYSTYQKIVYACSFGAEGIVLIDLISKVV
CCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KEADIVFLDTGLHFPETYELINEVKDRYPGLSIHFLKPGLSLEEQEKRYGSELWKRQPDQ
HHCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHH
CCRLRKIEPLREHLSGMEAWITGLRREQSETRKHVRYINKDDNFKLIKICPLIHWTWEDV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHEECHHHH
WTYIKLNQLTYHKLHDQDYPSIGCEVCTLPVTDSGNLRAGRWAHQEKTECGLHQFR
HHHHHHCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]