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The map label for this gene is pflB [H]

Identifier: 52785883

GI number: 52785883

Start: 2077864

End: 2080089

Strand: Reverse

Name: pflB [H]

Synonym: BLi02132

Alternate gene names: 52785883

Gene position: 2080089-2077864 (Counterclockwise)

Preceding gene: 52785884

Following gene: 52785882

Centisome position: 49.26

GC content: 48.65

Gene sequence:

>2226_bases
ATGGAACAATGGAAAGGTTTCACCACAAACGTTTGGCAAAAAGAAGTCAATGTCCGCGATTTTATTCTCTCGAACTTTGA
GCCATATCAAGGTGACGAATCGTTTCTCGAACCTCCGACGGAAGCTACATCGGCATTATGGGATCATGTAATGGATTTGA
CAAAAAAAGAGCGTGAAAACGGAGGCGTCCTTGATATGGATACAGAGATCGTCTCAACGATCACCTCGCACGGTCCCGGA
TATTTGAACAAAGACCTGGAAAAAGTCGTCGGCGTTCAAACCGATGAGCCGTTTAAACGGTCGCTTCAGCCTTTCGGCGG
CATCCGAATGGCAAAGCAGGCATGCGAATCCTATGGTTTTAAACTGAATGAAGAAGTGGAAAGGATCTTTACCGATTACC
GCAAAACTCATAACCAAGGCGTGTTTGACGCATATACGGACGAAATGAAGCTCGCCCGAAAAGTCGGAATCATTACCGGA
CTGCCTGATGCTTACGGGCGCGGGCGCATCATCGGTGATTACCGGAGAGTGGCGCTTTACGGCGTGGATTTCTTGATCGA
TGAAAAGAAAAAAGATGCGGCCGGCACCTCTCGGGTGATGTCTGAAGAAAACATCCGCCTTCGTGAAGAACTGTCAGAAC
AAATCCGAGCATTGAACGAACTTAAAGCGCTTGCAAAAAGCTATGGGTTTGACATTTCCAAGCCTGCGGCGAATGCAAGA
GAAGCATTTCAATGGCTGTATTTTGCCTATTTGGCTGCCATTAAAGAGCAAAACGGAGCAGCAATGAGCCTTGGCCGCGT
GTCCACGTTCCTTGATATTTACATCGAAAGAGATTTGAAAACGGGCGTATTAACAGAGCGTGAAGCCCAAGAGCTTGTCG
ACCATTTCGTCATGAAGCTGCGTTTGGTCAAATTCGCGCGCACACCTGACTACAATGAACTGTTCAGCGGCGATCCGACG
TGGGTGACAGAATCAATCGGCGGAATGGCGCACGACGGACGCGCCCTGGTGACGAAAAACTCGTTCCGTTTCCTGCATAC
GCTTGACAATTTAGGCCCGGCGCCTGAACCGAATTTAACCGTTCTTTGGTCTGTCAGACTGCCGCAAAAGTTTAAAAACT
ACTGTGCCAAAATGTCGATTAAAACAAGCTCGATCCAATACGAAAATGACGATATCATGCGTCCAGAATACGGTGATGAC
TACGGAATCGCCTGCTGTGTATCGGCAATGGCAATCGGCAAACAAATGCAGTTCTTCGGAGCACGCGCCAACTTGGCGAA
AGCTCTTTTATATGCGATTAACGGCGGAAAAGACGAAAAGCATAAAATGCAAGTCGGTCCGGAAATGCCGCCGGTTGCTT
CCGACGTGCTGGACTATGACGAAGTGATGCATAAATTCGATCAGACGATGGAATGGCTCGCAGGCTTGTACATCAACACG
CTCAATGTCATTCACTACATGCATGATAAATATTGCTATGAAAGAATTGAAATGGCCCTGCACGACACGGAAATTTTGCG
GACGATGGCCACTGGGATCGCCGGCTTGAGTGTTGTCGCCGATTCATTAAGCGCTGTCAAATATGCCAAAGTCAGCGTGG
TCCGCGATGAAAACGGCATTGCGGTCGATTTTGAAACAGAAGGCGACTTTCCTAAGTACGGAAATAACGATGACCGCGTC
GACGCGATCGCCGTTGACATTGTCAAGCGCTTTATGAAAAAACTGCGCAAGCATCAGACATATCGCCAGTCCGTTCAGAC
CATGTCAATTTTAACGATCACGTCAAACGTCGTTTACGGCAAGAAAACCGGAAATACGCCGGATGGACGCCGCGCGGGAG
AACCGTTTGCTCCAGGTGCGAATCCGATGCACGGCCGCGATACTAAAGGGACGCTTGCATCGCTGTCTTCAGTGGCAAAG
CTGCCTTACAGCTATGCGCTCGACGGCATTTCCAACACCTTTTCAATCGTCCCGAAAGCGCTTGGCAAAGACGAAGAGAG
CCGCGCCGCCAATTTGTCAAGCATCCTTGACGGATATGCCGCAAAAACAGGACATCACTTAAATGTAAACGTATTTAACA
GAGAGACACTGCTCGACGCCATGGAACATCCAGAGGAATATCCGCAGTTAACGATTCGCGTCTCAGGCTATGCGGTCAAC
TTTATTAAGCTGACGAAAGAACAGCAGTTAGACGTCATCAGCAGAACCTTCCATGAATCGATGTAG

Upstream 100 bases:

>100_bases
AATATGTGATGTTTATCACATTCAAACCTGTTATATTCAACATAAGATATAAATGCAACAACTGTTATATCATTTCAACT
TGAAAAAGAGGGGGATTTAA

Downstream 100 bases:

>100_bases
CGAGCAATAAGCGGAGCGCGACCAAGGCGCTCCGCCTCATAAAAAGAGGTGATCTCCATGGATGGAAATATTCATTCGAT
CGAAACATTCGGCACCGTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 741; Mature: 741

Protein sequence:

>741_residues
MEQWKGFTTNVWQKEVNVRDFILSNFEPYQGDESFLEPPTEATSALWDHVMDLTKKERENGGVLDMDTEIVSTITSHGPG
YLNKDLEKVVGVQTDEPFKRSLQPFGGIRMAKQACESYGFKLNEEVERIFTDYRKTHNQGVFDAYTDEMKLARKVGIITG
LPDAYGRGRIIGDYRRVALYGVDFLIDEKKKDAAGTSRVMSEENIRLREELSEQIRALNELKALAKSYGFDISKPAANAR
EAFQWLYFAYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKTGVLTEREAQELVDHFVMKLRLVKFARTPDYNELFSGDPT
WVTESIGGMAHDGRALVTKNSFRFLHTLDNLGPAPEPNLTVLWSVRLPQKFKNYCAKMSIKTSSIQYENDDIMRPEYGDD
YGIACCVSAMAIGKQMQFFGARANLAKALLYAINGGKDEKHKMQVGPEMPPVASDVLDYDEVMHKFDQTMEWLAGLYINT
LNVIHYMHDKYCYERIEMALHDTEILRTMATGIAGLSVVADSLSAVKYAKVSVVRDENGIAVDFETEGDFPKYGNNDDRV
DAIAVDIVKRFMKKLRKHQTYRQSVQTMSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGTLASLSSVAK
LPYSYALDGISNTFSIVPKALGKDEESRAANLSSILDGYAAKTGHHLNVNVFNRETLLDAMEHPEEYPQLTIRVSGYAVN
FIKLTKEQQLDVISRTFHESM

Sequences:

>Translated_741_residues
MEQWKGFTTNVWQKEVNVRDFILSNFEPYQGDESFLEPPTEATSALWDHVMDLTKKERENGGVLDMDTEIVSTITSHGPG
YLNKDLEKVVGVQTDEPFKRSLQPFGGIRMAKQACESYGFKLNEEVERIFTDYRKTHNQGVFDAYTDEMKLARKVGIITG
LPDAYGRGRIIGDYRRVALYGVDFLIDEKKKDAAGTSRVMSEENIRLREELSEQIRALNELKALAKSYGFDISKPAANAR
EAFQWLYFAYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKTGVLTEREAQELVDHFVMKLRLVKFARTPDYNELFSGDPT
WVTESIGGMAHDGRALVTKNSFRFLHTLDNLGPAPEPNLTVLWSVRLPQKFKNYCAKMSIKTSSIQYENDDIMRPEYGDD
YGIACCVSAMAIGKQMQFFGARANLAKALLYAINGGKDEKHKMQVGPEMPPVASDVLDYDEVMHKFDQTMEWLAGLYINT
LNVIHYMHDKYCYERIEMALHDTEILRTMATGIAGLSVVADSLSAVKYAKVSVVRDENGIAVDFETEGDFPKYGNNDDRV
DAIAVDIVKRFMKKLRKHQTYRQSVQTMSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGTLASLSSVAK
LPYSYALDGISNTFSIVPKALGKDEESRAANLSSILDGYAAKTGHHLNVNVFNRETLLDAMEHPEEYPQLTIRVSGYAVN
FIKLTKEQQLDVISRTFHESM
>Mature_741_residues
MEQWKGFTTNVWQKEVNVRDFILSNFEPYQGDESFLEPPTEATSALWDHVMDLTKKERENGGVLDMDTEIVSTITSHGPG
YLNKDLEKVVGVQTDEPFKRSLQPFGGIRMAKQACESYGFKLNEEVERIFTDYRKTHNQGVFDAYTDEMKLARKVGIITG
LPDAYGRGRIIGDYRRVALYGVDFLIDEKKKDAAGTSRVMSEENIRLREELSEQIRALNELKALAKSYGFDISKPAANAR
EAFQWLYFAYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKTGVLTEREAQELVDHFVMKLRLVKFARTPDYNELFSGDPT
WVTESIGGMAHDGRALVTKNSFRFLHTLDNLGPAPEPNLTVLWSVRLPQKFKNYCAKMSIKTSSIQYENDDIMRPEYGDD
YGIACCVSAMAIGKQMQFFGARANLAKALLYAINGGKDEKHKMQVGPEMPPVASDVLDYDEVMHKFDQTMEWLAGLYINT
LNVIHYMHDKYCYERIEMALHDTEILRTMATGIAGLSVVADSLSAVKYAKVSVVRDENGIAVDFETEGDFPKYGNNDDRV
DAIAVDIVKRFMKKLRKHQTYRQSVQTMSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGTLASLSSVAK
LPYSYALDGISNTFSIVPKALGKDEESRAANLSSILDGYAAKTGHHLNVNVFNRETLLDAMEHPEEYPQLTIRVSGYAVN
FIKLTKEQQLDVISRTFHESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=749, Percent_Identity=66.0881174899867, Blast_Score=1052, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=63.9147802929427, Blast_Score=1014, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=575, Percent_Identity=27.8260869565217, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1790388, Length=685, Percent_Identity=24.963503649635, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1788933, Length=58, Percent_Identity=74.1379310344828, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 83432; Mature: 83432

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQWKGFTTNVWQKEVNVRDFILSNFEPYQGDESFLEPPTEATSALWDHVMDLTKKEREN
CCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCC
GGVLDMDTEIVSTITSHGPGYLNKDLEKVVGVQTDEPFKRSLQPFGGIRMAKQACESYGF
CCEEEECHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHCCC
KLNEEVERIFTDYRKTHNQGVFDAYTDEMKLARKVGIITGLPDAYGRGRIIGDYRRVALY
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
GVDFLIDEKKKDAAGTSRVMSEENIRLREELSEQIRALNELKALAKSYGFDISKPAANAR
HHHHEEECCHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EAFQWLYFAYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKTGVLTEREAQELVDHFVMKL
HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
RLVKFARTPDYNELFSGDPTWVTESIGGMAHDGRALVTKNSFRFLHTLDNLGPAPEPNLT
HHHHHHCCCCHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCEE
VLWSVRLPQKFKNYCAKMSIKTSSIQYENDDIMRPEYGDDYGIACCVSAMAIGKQMQFFG
EEEEECCCHHHHHHHHHCCEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ARANLAKALLYAINGGKDEKHKMQVGPEMPPVASDVLDYDEVMHKFDQTMEWLAGLYINT
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVIHYMHDKYCYERIEMALHDTEILRTMATGIAGLSVVADSLSAVKYAKVSVVRDENGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
AVDFETEGDFPKYGNNDDRVDAIAVDIVKRFMKKLRKHQTYRQSVQTMSILTITSNVVYG
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEC
KKTGNTPDGRRAGEPFAPGANPMHGRDTKGTLASLSSVAKLPYSYALDGISNTFSIVPKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHH
LGKDEESRAANLSSILDGYAAKTGHHLNVNVFNRETLLDAMEHPEEYPQLTIRVSGYAVN
HCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEECEEEE
FIKLTKEQQLDVISRTFHESM
EEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEQWKGFTTNVWQKEVNVRDFILSNFEPYQGDESFLEPPTEATSALWDHVMDLTKKEREN
CCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCC
GGVLDMDTEIVSTITSHGPGYLNKDLEKVVGVQTDEPFKRSLQPFGGIRMAKQACESYGF
CCEEEECHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHCCC
KLNEEVERIFTDYRKTHNQGVFDAYTDEMKLARKVGIITGLPDAYGRGRIIGDYRRVALY
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
GVDFLIDEKKKDAAGTSRVMSEENIRLREELSEQIRALNELKALAKSYGFDISKPAANAR
HHHHEEECCHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EAFQWLYFAYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKTGVLTEREAQELVDHFVMKL
HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
RLVKFARTPDYNELFSGDPTWVTESIGGMAHDGRALVTKNSFRFLHTLDNLGPAPEPNLT
HHHHHHCCCCHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCEE
VLWSVRLPQKFKNYCAKMSIKTSSIQYENDDIMRPEYGDDYGIACCVSAMAIGKQMQFFG
EEEEECCCHHHHHHHHHCCEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ARANLAKALLYAINGGKDEKHKMQVGPEMPPVASDVLDYDEVMHKFDQTMEWLAGLYINT
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVIHYMHDKYCYERIEMALHDTEILRTMATGIAGLSVVADSLSAVKYAKVSVVRDENGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
AVDFETEGDFPKYGNNDDRVDAIAVDIVKRFMKKLRKHQTYRQSVQTMSILTITSNVVYG
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEC
KKTGNTPDGRRAGEPFAPGANPMHGRDTKGTLASLSSVAKLPYSYALDGISNTFSIVPKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHH
LGKDEESRAANLSSILDGYAAKTGHHLNVNVFNRETLLDAMEHPEEYPQLTIRVSGYAVN
HCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEECEEEE
FIKLTKEQQLDVISRTFHESM
EEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA