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The map label for this gene is yhjG [H]

Identifier: 52785455

GI number: 52785455

Start: 1650841

End: 1652322

Strand: Reverse

Name: yhjG [H]

Synonym: BLi01695

Alternate gene names: 52785455

Gene position: 1652322-1650841 (Counterclockwise)

Preceding gene: 52785456

Following gene: 52785454

Centisome position: 39.13

GC content: 52.77

Gene sequence:

>1482_bases
ATGGATTTTGATGTATTGATTGCAGGCGGCGGCCCCGTCGGTCTGCTGACGGCTTCCGAGCTGGCTTTGGCCGGCGTCCG
GACCTGTATTGTCGAACGGCTGAATGAGCCTTCTCCATTCTCAAAAGCGCTCACACTGCATCCTCGCTCAATCGAAATAC
TTGAAATGAGGGGGCTTGGAGAACGATTTAAGGAAATTGGCAAGCCGCTCGGTTCGGGGCACTTTGCAGGTCTGAAGACT
GGATTGGATTTTTCTGCGCTTGACACGCGAGCCAATTTTACACTGTTCATCCCCCAGTACGAAACTGAGAAGGTGCTTGA
GGAACACGCGAGGAATCTCGGTGTCTGCATCCTTCGCGGTCATGAAGTTCTCGCTGTCCGCGAGCATGAAGACACCGCCG
AGCTTGTCGTCCAGGGTCCGGACGGCCTGTCCTTACTAACCGCTTCCTATATCGTCGGTGCCGACGGGGCCGGAAGTACC
ATCCGCAAGCAGGCGCGCATCGCTTTTTCCGGAACTGATGCGAATATCACCGCAATGCTTGGTGATGTGTCGCTTGAAAA
TCCGCCGGAATCGCATTTCTATACTTGCGTGAACCGTGAAGGCGGCGTTGTGATCGTGCCTTTAGACGGCGGCATTTTCC
GCGTCCTCATAACGGCGCCGCATATGCCCCAAGCTTTGCTTCACGAGCCCGTCACACTGGAAGAATTAAAAAAAGAACTC
AGCCGCATTTGCGGTACGGACTTTGGAATTAACAGCCCGGTTTGGATGTCCCGCTTCGGAAATGCCAGCAAGTTGGCGGA
GCATTACCGTTCCGGCAGAATTTTTTTAGCTGGAGATGCCGCGCATATACATTTTCCCGCAGGTGGGCAAGGTTTAAATG
TCGGGCTTCAAGATGCGATGAACCTTGGCTGGAAGCTCGCAGCAAAGATCAACGGACGGTGTCCCGGCTGGCTGCTTGAC
AGTTACCATCATGAAAGACACCCTGTCGGCAAAGCGCTGATCGTCAATACACAAATCCAAACAAAGCTGTTTGATTTTAC
GCGTGAAGGGCTGTACCTCCGTGAATTGCTATCCGGCATACTCGGATTTTCAGACGTCAATCGCTATCTGGCAGAACAAA
TTGCAGCGCTCAGCGTGCAGTATGAAGCAGATGAAAAAATGCCTGAACATGAGCTGAACGGTAAACGCCTGCCCGATATT
GACCTGACCCTTTCAGATGGTACTGTCAAACGACTTTACACATTTCACCGCGATGGACGTTTCTTATTAGCAGCATTCGG
AAAAGAACCGAAGGAATTTTCCAGACTGCCTCATGTCCGGCTCATGCAACTGAAAGCGATTGACGGACGGCCTGAATGGA
ATGGCGTAAAAGCAGCCCTGATCCGCCCCGACGGCCACATAGCCTGGGCTGTGGACGAATCCCTGGAAAACGCGGAGCAA
TTGATTGCCGATGGAATCCGCCGCTGGTGCGGAAACGCTTAA

Upstream 100 bases:

>100_bases
GTTACATAAGTAACAAAAATAATAGAATATAGTTGATAAAAATTTTCATTTGTAGTATTTTAAAATTGTTTCATACGCAA
CAAAAAAGGAGTGATTGATC

Downstream 100 bases:

>100_bases
TCTGCAATCTGTTTAACCATTTTGTTTCACGCGTAACAAAATGGTATAATCAAAGCAGAATAATCGATTAAAGGAGCCGG
TTTTCTGCATGGAACATTTA

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MDFDVLIAGGGPVGLLTASELALAGVRTCIVERLNEPSPFSKALTLHPRSIEILEMRGLGERFKEIGKPLGSGHFAGLKT
GLDFSALDTRANFTLFIPQYETEKVLEEHARNLGVCILRGHEVLAVREHEDTAELVVQGPDGLSLLTASYIVGADGAGST
IRKQARIAFSGTDANITAMLGDVSLENPPESHFYTCVNREGGVVIVPLDGGIFRVLITAPHMPQALLHEPVTLEELKKEL
SRICGTDFGINSPVWMSRFGNASKLAEHYRSGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAKINGRCPGWLLD
SYHHERHPVGKALIVNTQIQTKLFDFTREGLYLRELLSGILGFSDVNRYLAEQIAALSVQYEADEKMPEHELNGKRLPDI
DLTLSDGTVKRLYTFHRDGRFLLAAFGKEPKEFSRLPHVRLMQLKAIDGRPEWNGVKAALIRPDGHIAWAVDESLENAEQ
LIADGIRRWCGNA

Sequences:

>Translated_493_residues
MDFDVLIAGGGPVGLLTASELALAGVRTCIVERLNEPSPFSKALTLHPRSIEILEMRGLGERFKEIGKPLGSGHFAGLKT
GLDFSALDTRANFTLFIPQYETEKVLEEHARNLGVCILRGHEVLAVREHEDTAELVVQGPDGLSLLTASYIVGADGAGST
IRKQARIAFSGTDANITAMLGDVSLENPPESHFYTCVNREGGVVIVPLDGGIFRVLITAPHMPQALLHEPVTLEELKKEL
SRICGTDFGINSPVWMSRFGNASKLAEHYRSGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAKINGRCPGWLLD
SYHHERHPVGKALIVNTQIQTKLFDFTREGLYLRELLSGILGFSDVNRYLAEQIAALSVQYEADEKMPEHELNGKRLPDI
DLTLSDGTVKRLYTFHRDGRFLLAAFGKEPKEFSRLPHVRLMQLKAIDGRPEWNGVKAALIRPDGHIAWAVDESLENAEQ
LIADGIRRWCGNA
>Mature_493_residues
MDFDVLIAGGGPVGLLTASELALAGVRTCIVERLNEPSPFSKALTLHPRSIEILEMRGLGERFKEIGKPLGSGHFAGLKT
GLDFSALDTRANFTLFIPQYETEKVLEEHARNLGVCILRGHEVLAVREHEDTAELVVQGPDGLSLLTASYIVGADGAGST
IRKQARIAFSGTDANITAMLGDVSLENPPESHFYTCVNREGGVVIVPLDGGIFRVLITAPHMPQALLHEPVTLEELKKEL
SRICGTDFGINSPVWMSRFGNASKLAEHYRSGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAKINGRCPGWLLD
SYHHERHPVGKALIVNTQIQTKLFDFTREGLYLRELLSGILGFSDVNRYLAEQIAALSVQYEADEKMPEHELNGKRLPDI
DLTLSDGTVKRLYTFHRDGRFLLAAFGKEPKEFSRLPHVRLMQLKAIDGRPEWNGVKAALIRPDGHIAWAVDESLENAEQ
LIADGIRRWCGNA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=422, Percent_Identity=27.4881516587678, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789273, Length=322, Percent_Identity=25.1552795031056, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786883, Length=336, Percent_Identity=24.4047619047619, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54127; Mature: 54127

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDVLIAGGGPVGLLTASELALAGVRTCIVERLNEPSPFSKALTLHPRSIEILEMRGLG
CCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCHH
ERFKEIGKPLGSGHFAGLKTGLDFSALDTRANFTLFIPQYETEKVLEEHARNLGVCILRG
HHHHHHCCCCCCCCCCHHCCCCCCHHCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEC
HEVLAVREHEDTAELVVQGPDGLSLLTASYIVGADGAGSTIRKQARIAFSGTDANITAML
CEEEEEECCCCCEEEEEECCCCCCEEEEHHEEECCCCCHHHHHCEEEEEECCCCEEEEEE
GDVSLENPPESHFYTCVNREGGVVIVPLDGGIFRVLITAPHMPQALLHEPVTLEELKKEL
ECCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHCCCCCHHHHHHHH
SRICGTDFGINSPVWMSRFGNASKLAEHYRSGRIFLAGDAAHIHFPAGGQGLNVGLQDAM
HHHCCCCCCCCCCHHHHHCCCHHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCCHHHHH
NLGWKLAAKINGRCPGWLLDSYHHERHPVGKALIVNTQIQTKLFDFTREGLYLRELLSGI
CCCEEEEEEECCCCCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHH
LGFSDVNRYLAEQIAALSVQYEADEKMPEHELNGKRLPDIDLTLSDGTVKRLYTFHRDGR
HCHHHHHHHHHHHHHHHEEEECCCCCCCCHHCCCCCCCCEEEEECCCCEEEHHEEECCCC
FLLAAFGKEPKEFSRLPHVRLMQLKAIDGRPEWNGVKAALIRPDGHIAWAVDESLENAEQ
EEEEECCCCHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEECHHHHHHHH
LIADGIRRWCGNA
HHHHHHHHHHCCC
>Mature Secondary Structure
MDFDVLIAGGGPVGLLTASELALAGVRTCIVERLNEPSPFSKALTLHPRSIEILEMRGLG
CCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCHH
ERFKEIGKPLGSGHFAGLKTGLDFSALDTRANFTLFIPQYETEKVLEEHARNLGVCILRG
HHHHHHCCCCCCCCCCHHCCCCCCHHCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEC
HEVLAVREHEDTAELVVQGPDGLSLLTASYIVGADGAGSTIRKQARIAFSGTDANITAML
CEEEEEECCCCCEEEEEECCCCCCEEEEHHEEECCCCCHHHHHCEEEEEECCCCEEEEEE
GDVSLENPPESHFYTCVNREGGVVIVPLDGGIFRVLITAPHMPQALLHEPVTLEELKKEL
ECCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHCCCCCHHHHHHHH
SRICGTDFGINSPVWMSRFGNASKLAEHYRSGRIFLAGDAAHIHFPAGGQGLNVGLQDAM
HHHCCCCCCCCCCHHHHHCCCHHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCCHHHHH
NLGWKLAAKINGRCPGWLLDSYHHERHPVGKALIVNTQIQTKLFDFTREGLYLRELLSGI
CCCEEEEEEECCCCCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHH
LGFSDVNRYLAEQIAALSVQYEADEKMPEHELNGKRLPDIDLTLSDGTVKRLYTFHRDGR
HCHHHHHHHHHHHHHHHEEEECCCCCCCCHHCCCCCCCCEEEEECCCCEEEHHEEECCCC
FLLAAFGKEPKEFSRLPHVRLMQLKAIDGRPEWNGVKAALIRPDGHIAWAVDESLENAEQ
EEEEECCCCHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEECHHHHHHHH
LIADGIRRWCGNA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]