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The map label for this gene is def [H]

Identifier: 52785431

GI number: 52785431

Start: 1630527

End: 1631084

Strand: Reverse

Name: def [H]

Synonym: BLi01671

Alternate gene names: 52785431

Gene position: 1631084-1630527 (Counterclockwise)

Preceding gene: 52785432

Following gene: 52785429

Centisome position: 38.63

GC content: 48.21

Gene sequence:

>558_bases
ATGTTGATCACAATGGAAAACATTGTCCGCGACGGTCATCCTGCTTTGAGAACCGTCGCCGAAGAAGTTCAATTGCCAGC
AAGCGAAGAAGAAATCAAACAGCTGAGCGAAATGATCGAGTTTGTCAAAAACAGCCAGGACCCCGAAATCGCTAAAAAGT
ACAACCTTCGTCCTGGTGTCGGTCTAGCCGCTCCGCAAATTAATATCAATAAACGAATGATCGCCGTTCATGCCGAAAGC
GAAGACGGGACACTATACAGCTATGCGCTTTTCAATCCGAAGATCGTCAGCCACTCTGTTCAAAGATGCTATTTGACAAG
CGGCGAGGGCTGCCTGTCCGTCGACGAGCCGATTCCCGGATATGTTCCGCGCTATGAACGGATCCGAGTCAAAGGAACGA
CGCTTGAAGGCGAAAACATCGACATCCGCTTAAAAGGATATCCGGCGATCGTATTCCAGCATGAGATCGATCATTTGAAC
GGCATTATGTTTTACGACCACATCGACAAAGAAAACCCGCTGAAACAACCTGAAAATGCAACGCCTGTAGGCAGGTAA

Upstream 100 bases:

>100_bases
TGACCAGCCTCCTTTAGGCTGGTCATTTTATCATGCACAGAGATTGCGCGGAAAGAGCAAGATCCGCTATACTTTACATA
ATGAATCGTTGGGAGTGAAG

Downstream 100 bases:

>100_bases
AGTCATAAAAAAAACGGATCATTTCTGATCCGTTTTTTATGATTTCAAAAGTCCGAGCGCTTCCACCGCGTATTGAATGC
CGTCTTCATCGACGGGCTTT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MLITMENIVRDGHPALRTVAEEVQLPASEEEIKQLSEMIEFVKNSQDPEIAKKYNLRPGVGLAAPQININKRMIAVHAES
EDGTLYSYALFNPKIVSHSVQRCYLTSGEGCLSVDEPIPGYVPRYERIRVKGTTLEGENIDIRLKGYPAIVFQHEIDHLN
GIMFYDHIDKENPLKQPENATPVGR

Sequences:

>Translated_185_residues
MLITMENIVRDGHPALRTVAEEVQLPASEEEIKQLSEMIEFVKNSQDPEIAKKYNLRPGVGLAAPQININKRMIAVHAES
EDGTLYSYALFNPKIVSHSVQRCYLTSGEGCLSVDEPIPGYVPRYERIRVKGTTLEGENIDIRLKGYPAIVFQHEIDHLN
GIMFYDHIDKENPLKQPENATPVGR
>Mature_185_residues
MLITMENIVRDGHPALRTVAEEVQLPASEEEIKQLSEMIEFVKNSQDPEIAKKYNLRPGVGLAAPQININKRMIAVHAES
EDGTLYSYALFNPKIVSHSVQRCYLTSGEGCLSVDEPIPGYVPRYERIRVKGTTLEGENIDIRLKGYPAIVFQHEIDHLN
GIMFYDHIDKENPLKQPENATPVGR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=119, Percent_Identity=36.1344537815126, Blast_Score=72, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645728, Length=178, Percent_Identity=30.8988764044944, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20870; Mature: 20870

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLITMENIVRDGHPALRTVAEEVQLPASEEEIKQLSEMIEFVKNSQDPEIAKKYNLRPGV
CEEEHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
GLAAPQININKRMIAVHAESEDGTLYSYALFNPKIVSHSVQRCYLTSGEGCLSVDEPIPG
CEECCEEECCCEEEEEEECCCCCEEEEEEECCCHHHHHCCCEEEEECCCCCEEECCCCCC
YVPRYERIRVKGTTLEGENIDIRLKGYPAIVFQHEIDHLNGIMFYDHIDKENPLKQPENA
CCCCCEEEEEEECEECCCCEEEEECCCEEEEEEECHHCCCCEEEEECCCCCCCCCCCCCC
TPVGR
CCCCC
>Mature Secondary Structure
MLITMENIVRDGHPALRTVAEEVQLPASEEEIKQLSEMIEFVKNSQDPEIAKKYNLRPGV
CEEEHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
GLAAPQININKRMIAVHAESEDGTLYSYALFNPKIVSHSVQRCYLTSGEGCLSVDEPIPG
CEECCEEECCCEEEEEEECCCCCEEEEEEECCCHHHHHCCCEEEEECCCCCEEECCCCCC
YVPRYERIRVKGTTLEGENIDIRLKGYPAIVFQHEIDHLNGIMFYDHIDKENPLKQPENA
CCCCCEEEEEEECEECCCCEEEEECCCEEEEEEECHHCCCCEEEEECCCCCCCCCCCCCC
TPVGR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11429456 [H]