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The map label for this gene is clpE [H]

Identifier: 52785290

GI number: 52785290

Start: 1498277

End: 1500373

Strand: Reverse

Name: clpE [H]

Synonym: BLi01525

Alternate gene names: 52785290

Gene position: 1500373-1498277 (Counterclockwise)

Preceding gene: 52785299

Following gene: 52785289

Centisome position: 35.53

GC content: 47.69

Gene sequence:

>2097_bases
ATGCGTTGTCAACATTGTCAAAAAAATGAGGCGACGATTCGCCTAAACATGCAAATCAACTTTACAAAAAAACAAATGGA
TTTATGTGAAGACTGCTATAAAGAGCTGACTCAGCCTTCTATGTTTTCAGGAAATCACTTTTTTGGCGGCGGAGGTTCTT
TCTTCAGCCAGCCATCACAAGCGCAGACGCAAACCGCTGCCAAAAAAGGCTTGCTCGATGAACTTGCCGTCAATTTAACA
AATGCAGCAAAGACCGGCTTGATCGATCCGGTGATCGGCCGCGATGATGAAGTAGCCCGTGTGATCGAGATCTTAAACCG
CAGAAATAAAAACAATCCGGTCTTAATCGGTGAACCGGGCGTCGGTAAAACAGCCGTCGCTGAAGGACTTGCTCTCAAAA
TCGCCGAAGGCGATGTTCCAAATAAATTGAAAAACAAAGAGCTCTACCTGCTTGATGTCGCTTCCCTCGTGGCAAACACC
GGCATCAGAGGGCAATTTGAAGAAAGAATGAAACAGCTGATGAAAGAGCTGAAAGAACGCAAAAACGCAATCTTGTTTAT
TGACGAAATTCACCTTCTTGTCGGGGCCGGCTCTGCAGAAGGCTCCATGGATGCGGGCAACATTTTAAAACCTGCACTTG
CCAGAGGCGAGCTGCAAGTTATCGGCGCTACGACGTTGAAGGAATATCGCCAAATTGAAAAAGATGCGGCGTTAGAGCGG
CGCTTTCAGCCGATCATGGTGCACGAACCGACAATTGAGCAGGCCGCAGCGATTTTAAACGGGCTCAAGGAAAAATACGA
AGCATATCACGATGTGACTTACACGGATGAAGCGATCAAAGCCTGCGTCATTCTGTCAAGCCGCTATATTCAGGACCGCC
ATCTTCCCGATAAGGCGATCGACCTTTTGGATGAAGCCGGTTCAAAAGCGAACTTGAAAATCGACACGGTCAGCGGTGAA
CAAGCTTCCCAACGCCTGCAAGAAATCGAAGCGGAAAAAGCCAAAGCGCTTCAAGAAGAAAACTACGAGCTTGCAGCAAA
ACTCCGCGACGAAGAAGAAGCGCTGCATAAAAAAATGGAAAGCCATGAGGAAGAAAAACGGGCAACAGTTGAAGCGGAAG
ACATTCAAGCGATCATCGAACAAAAAACCGGCATTCCGGTCGGCAAGCTTCAGCACGACGATCAGAAGAAAATGAAAGAG
CTTGAAGCACACCTTTCAAGCCGCGTGATCGGTCAAAAAGAAGCGGTGAAAAAAGTGGCAAAAGCCGTCAGAAGAAGCCG
CGCCGGCCTGAAATCAAAAAACAGGCCTGTCGGTTCATTCCTGTTCGTCGGCCCGACAGGTGTCGGTAAAACAGAACTTT
CTAAAAGGCTTGCGGAAGAACTGTTCGGCACGAAGGATGCCATCATCCGCCTCGATATGAGCGAATACATGGAAAAACAC
GCCGTATCAAAGCTGATCGGTTCGCCTCCTGGATATGTCGGCCACGAAGAAGCCGGACAGCTTACCGAAAAAGTCCGGAG
GAACCCGTACAGCATCGTTTTGCTCGATGAAATCGAAAAAGCCCATCCCGATGTCCAGCACATGTTCCTGCAAATCATGG
AAGACGGACGTCTGACAGACAGCCAAGGGCGGACGGTCAGCTTTAAAGATACCGTCATTATTATGACAAGCAATGCCGGT
GTATCTGAAAAGCGGACAACCGTCGGTTTTAACCGGGAAGAGCAAGTAATGGGCGAACAATCAATGATCGATTCATTAAG
CGGCTACTTTAAGCCGGAATTCCTGAACAGATTTGACAGCATCATCGAATTCCAGCCGCTCGAAAAAGAAGATCTGGTCA
AGATCGCCGCCCTCTTGCTGAAAGAGCTTGAAGAAACGTTAAAAGAGCAAAACATGACGCTCGAAGTTTCAGATGAGGCA
AAAGAAAAAATCGCTGAAATCGGATACCATCCGGCCTTTGGCGCCCGCCCATTAAGAAGAACCATTCAAACAGCGGTCGA
AGATCAAATGACCGATCTGCTGCTTGAAGAAGAACACATCAAAGGTTTTGCGGTTATCGTTGAAAACGATGAAATCCGGG
TGAAAACAACCAGATAA

Upstream 100 bases:

>100_bases
GTCAATGTATACTAATAGTCAGAAATGGTCAAACTGCAGACCATTTTTTCTAAGTCAATTGGTCAAAAAAGGTCAAATTC
AATAAAGGGGGTTTTGGCAA

Downstream 100 bases:

>100_bases
CACACACAACGGCCTCCGATCGCGGAGGTCGTTTTACATACAACATAAGAATCACAAAAGTTCTATTTTTTAACATTAAA
TAACCCTAAAGTCCCTTTTC

Product: ClpE

Products: NA

Alternate protein names: ATPase ClpE; Heat shock protein HSP1 [H]

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MRCQHCQKNEATIRLNMQINFTKKQMDLCEDCYKELTQPSMFSGNHFFGGGGSFFSQPSQAQTQTAAKKGLLDELAVNLT
NAAKTGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAVAEGLALKIAEGDVPNKLKNKELYLLDVASLVANT
GIRGQFEERMKQLMKELKERKNAILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALER
RFQPIMVHEPTIEQAAAILNGLKEKYEAYHDVTYTDEAIKACVILSSRYIQDRHLPDKAIDLLDEAGSKANLKIDTVSGE
QASQRLQEIEAEKAKALQEENYELAAKLRDEEEALHKKMESHEEEKRATVEAEDIQAIIEQKTGIPVGKLQHDDQKKMKE
LEAHLSSRVIGQKEAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKRLAEELFGTKDAIIRLDMSEYMEKH
AVSKLIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSNAG
VSEKRTTVGFNREEQVMGEQSMIDSLSGYFKPEFLNRFDSIIEFQPLEKEDLVKIAALLLKELEETLKEQNMTLEVSDEA
KEKIAEIGYHPAFGARPLRRTIQTAVEDQMTDLLLEEEHIKGFAVIVENDEIRVKTTR

Sequences:

>Translated_698_residues
MRCQHCQKNEATIRLNMQINFTKKQMDLCEDCYKELTQPSMFSGNHFFGGGGSFFSQPSQAQTQTAAKKGLLDELAVNLT
NAAKTGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAVAEGLALKIAEGDVPNKLKNKELYLLDVASLVANT
GIRGQFEERMKQLMKELKERKNAILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALER
RFQPIMVHEPTIEQAAAILNGLKEKYEAYHDVTYTDEAIKACVILSSRYIQDRHLPDKAIDLLDEAGSKANLKIDTVSGE
QASQRLQEIEAEKAKALQEENYELAAKLRDEEEALHKKMESHEEEKRATVEAEDIQAIIEQKTGIPVGKLQHDDQKKMKE
LEAHLSSRVIGQKEAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKRLAEELFGTKDAIIRLDMSEYMEKH
AVSKLIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSNAG
VSEKRTTVGFNREEQVMGEQSMIDSLSGYFKPEFLNRFDSIIEFQPLEKEDLVKIAALLLKELEETLKEQNMTLEVSDEA
KEKIAEIGYHPAFGARPLRRTIQTAVEDQMTDLLLEEEHIKGFAVIVENDEIRVKTTR
>Mature_698_residues
MRCQHCQKNEATIRLNMQINFTKKQMDLCEDCYKELTQPSMFSGNHFFGGGGSFFSQPSQAQTQTAAKKGLLDELAVNLT
NAAKTGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAVAEGLALKIAEGDVPNKLKNKELYLLDVASLVANT
GIRGQFEERMKQLMKELKERKNAILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALER
RFQPIMVHEPTIEQAAAILNGLKEKYEAYHDVTYTDEAIKACVILSSRYIQDRHLPDKAIDLLDEAGSKANLKIDTVSGE
QASQRLQEIEAEKAKALQEENYELAAKLRDEEEALHKKMESHEEEKRATVEAEDIQAIIEQKTGIPVGKLQHDDQKKMKE
LEAHLSSRVIGQKEAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKRLAEELFGTKDAIIRLDMSEYMEKH
AVSKLIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSNAG
VSEKRTTVGFNREEQVMGEQSMIDSLSGYFKPEFLNRFDSIIEFQPLEKEDLVKIAALLLKELEETLKEQNMTLEVSDEA
KEKIAEIGYHPAFGARPLRRTIQTAVEDQMTDLLLEEEHIKGFAVIVENDEIRVKTTR

Specific function: ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=36.9127516778523, Blast_Score=190, Evalue=5e-48,
Organism=Escherichia coli, GI1787109, Length=639, Percent_Identity=42.2535211267606, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1788943, Length=332, Percent_Identity=48.1927710843374, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6320464, Length=682, Percent_Identity=43.4017595307918, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6323002, Length=694, Percent_Identity=40.4899135446686, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 78100; Mature: 78100

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCQHCQKNEATIRLNMQINFTKKQMDLCEDCYKELTQPSMFSGNHFFGGGGSFFSQPSQ
CCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHCCCCCH
AQTQTAAKKGLLDELAVNLTNAAKTGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPG
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCC
VGKTAVAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLMKELKER
CCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KNAILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALER
CCEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
RFQPIMVHEPTIEQAAAILNGLKEKYEAYHDVTYTDEAIKACVILSSRYIQDRHLPDKAI
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
DLLDEAGSKANLKIDTVSGEQASQRLQEIEAEKAKALQEENYELAAKLRDEEEALHKKME
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHH
SHEEEKRATVEAEDIQAIIEQKTGIPVGKLQHDDQKKMKELEAHLSSRVIGQKEAVKKVA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
KAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKRLAEELFGTKDAIIRLDMSEYMEKH
HHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHH
AVSKLIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTD
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCC
SQGRTVSFKDTVIIMTSNAGVSEKRTTVGFNREEQVMGEQSMIDSLSGYFKPEFLNRFDS
CCCCEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IIEFQPLEKEDLVKIAALLLKELEETLKEQNMTLEVSDEAKEKIAEIGYHPAFGARPLRR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHHH
TIQTAVEDQMTDLLLEEEHIKGFAVIVENDEIRVKTTR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECC
>Mature Secondary Structure
MRCQHCQKNEATIRLNMQINFTKKQMDLCEDCYKELTQPSMFSGNHFFGGGGSFFSQPSQ
CCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHCCCCCH
AQTQTAAKKGLLDELAVNLTNAAKTGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPG
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCC
VGKTAVAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLMKELKER
CCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KNAILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALER
CCEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
RFQPIMVHEPTIEQAAAILNGLKEKYEAYHDVTYTDEAIKACVILSSRYIQDRHLPDKAI
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
DLLDEAGSKANLKIDTVSGEQASQRLQEIEAEKAKALQEENYELAAKLRDEEEALHKKME
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHH
SHEEEKRATVEAEDIQAIIEQKTGIPVGKLQHDDQKKMKELEAHLSSRVIGQKEAVKKVA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
KAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKRLAEELFGTKDAIIRLDMSEYMEKH
HHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHH
AVSKLIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTD
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCC
SQGRTVSFKDTVIIMTSNAGVSEKRTTVGFNREEQVMGEQSMIDSLSGYFKPEFLNRFDS
CCCCEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IIEFQPLEKEDLVKIAALLLKELEETLKEQNMTLEVSDEAKEKIAEIGYHPAFGARPLRR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHHH
TIQTAVEDQMTDLLLEEEHIKGFAVIVENDEIRVKTTR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]