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The map label for this gene is glpF [H]

Identifier: 52784766

GI number: 52784766

Start: 996539

End: 997354

Strand: Direct

Name: glpF [H]

Synonym: BLi00994

Alternate gene names: 52784766

Gene position: 996539-997354 (Clockwise)

Preceding gene: 52784765

Following gene: 52784767

Centisome position: 23.6

GC content: 47.67

Gene sequence:

>816_bases
TTGACAGCATTTTGGGGGGAAGTTATCGGAACGATGCTGCTCATCGTCTTTGGAGCTGGAGTTTGTGCAGGAGTTAATTT
GAAAAAATCGCTGTCCCATCAATCCGGATGGATTGTGATCGTCTTCGGCTGGGGGCTTGGCGTGGCCATGGCGGTATATG
CCGTCGGCGGCATCAGCGGAGCGCATTTAAATCCGGCCGTTACATTGGGGCTGGCATTTGTCGGAGATTTTCCTTGGGAA
GAAGTGCCTTCATATATTTTGGGACAGATGATCGGCGCATTTTTAGGAGCGGTGCTCGTTTTTCTTCACTACTTGCCGCA
CTGGAAAGAAACCGAGGATCAAGGCGCGAAGCTTGGAGTATTTTCGACAGGTCCGGCGATTCCAAATACATTTGCAAACC
TGTTCAGTGAAACATTGGGGACTTTTATTCTCGTTCTCGGACTTTTAACGATCGGTGCAAACAAGTTTACTGACGGACTG
AATCCTCTTGTTGTCGGATTTCTGATCGTGGCGATCGGTATCTCGCTCGGCGGAACAACAGGCTATGCGATTAACCCTGC
CCGCGATCTGGGGCCGAGAATTGCCCATTTTGTCCTTCCGATTGCAGGCAAAGGGAGTTCAAACTGGAAGTACGCGTGGA
TCCCTGTTTTAGGACCGGCGCTTGGCGGTTCATTTGCAGGCGTTTTTTACAACGCCGTATTCAAAGGGCATATCACAAAC
ACATTTTGGATTGTAAGCGTTATACTAGTTGTGATATTGTTAGGTTTCTATATTCATATGAAAAAACAAGCAGTTGATCA
ATCGGTCAACATTTAA

Upstream 100 bases:

>100_bases
CTGTGTTTTTTTATGGTCTTTTAGACACAGTGGAGAAGGTGAACTTATGGCGTTCATCTATTAGAATAATACTTCATAAT
AGATTTTAGGAGGGATAGCC

Downstream 100 bases:

>100_bases
AAAAAACGCAATCTTAACAGACATATAAGGGGGAGTTTCAAAATGGAAAAGTACATTTTGTCTCTTGATCAAGGCACCAC
AAGCACAAGGGCGATTGTTT

Product: hypothetical protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTAFWGEVIGTMLLIVFGAGVCAGVNLKKSLSHQSGWIVIVFGWGLGVAMAVYAVGGISGAHLNPAVTLGLAFVGDFPWE
EVPSYILGQMIGAFLGAVLVFLHYLPHWKETEDQGAKLGVFSTGPAIPNTFANLFSETLGTFILVLGLLTIGANKFTDGL
NPLVVGFLIVAIGISLGGTTGYAINPARDLGPRIAHFVLPIAGKGSSNWKYAWIPVLGPALGGSFAGVFYNAVFKGHITN
TFWIVSVILVVILLGFYIHMKKQAVDQSVNI

Sequences:

>Translated_271_residues
MTAFWGEVIGTMLLIVFGAGVCAGVNLKKSLSHQSGWIVIVFGWGLGVAMAVYAVGGISGAHLNPAVTLGLAFVGDFPWE
EVPSYILGQMIGAFLGAVLVFLHYLPHWKETEDQGAKLGVFSTGPAIPNTFANLFSETLGTFILVLGLLTIGANKFTDGL
NPLVVGFLIVAIGISLGGTTGYAINPARDLGPRIAHFVLPIAGKGSSNWKYAWIPVLGPALGGSFAGVFYNAVFKGHITN
TFWIVSVILVVILLGFYIHMKKQAVDQSVNI
>Mature_270_residues
TAFWGEVIGTMLLIVFGAGVCAGVNLKKSLSHQSGWIVIVFGWGLGVAMAVYAVGGISGAHLNPAVTLGLAFVGDFPWEE
VPSYILGQMIGAFLGAVLVFLHYLPHWKETEDQGAKLGVFSTGPAIPNTFANLFSETLGTFILVLGLLTIGANKFTDGLN
PLVVGFLIVAIGISLGGTTGYAINPARDLGPRIAHFVLPIAGKGSSNWKYAWIPVLGPALGGSFAGVFYNAVFKGHITNT
FWIVSVILVVILLGFYIHMKKQAVDQSVNI

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=250, Percent_Identity=36, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI157266307, Length=246, Percent_Identity=35.7723577235772, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI4502187, Length=242, Percent_Identity=37.603305785124, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI22538420, Length=249, Percent_Identity=32.9317269076305, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI310133356, Length=184, Percent_Identity=36.4130434782609, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI310114181, Length=184, Percent_Identity=36.4130434782609, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI37694062, Length=197, Percent_Identity=31.9796954314721, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4502179, Length=261, Percent_Identity=30.2681992337165, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4755125, Length=206, Percent_Identity=31.0679611650485, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4502181, Length=206, Percent_Identity=31.0679611650485, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=257, Percent_Identity=35.0194552529183, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994009, Length=246, Percent_Identity=34.1463414634146, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992966, Length=247, Percent_Identity=32.7935222672065, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=256, Percent_Identity=34.375, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17533613, Length=237, Percent_Identity=29.957805907173, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564052, Length=237, Percent_Identity=29.957805907173, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=30.5084745762712, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17531431, Length=235, Percent_Identity=29.7872340425532, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17531429, Length=235, Percent_Identity=29.7872340425532, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71992961, Length=242, Percent_Identity=30.9917355371901, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71993722, Length=232, Percent_Identity=30.6034482758621, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=37.8640776699029, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322985, Length=309, Percent_Identity=28.1553398058252, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652747, Length=235, Percent_Identity=34.468085106383, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45551084, Length=235, Percent_Identity=34.468085106383, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24762342, Length=244, Percent_Identity=26.6393442622951, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762344, Length=243, Percent_Identity=28.8065843621399, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24762346, Length=243, Percent_Identity=29.2181069958848, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24762348, Length=243, Percent_Identity=29.2181069958848, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20130305, Length=243, Percent_Identity=29.2181069958848, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922828, Length=192, Percent_Identity=31.7708333333333, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28649; Mature: 28518

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFWGEVIGTMLLIVFGAGVCAGVNLKKSLSHQSGWIVIVFGWGLGVAMAVYAVGGISG
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCC
AHLNPAVTLGLAFVGDFPWEEVPSYILGQMIGAFLGAVLVFLHYLPHWKETEDQGAKLGV
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FSTGPAIPNTFANLFSETLGTFILVLGLLTIGANKFTDGLNPLVVGFLIVAIGISLGGTT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFVLPIAGKGSSNWKYAWIPVLGPALGGSFAGVFYNAVFKGHITN
CCEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHCCCCH
TFWIVSVILVVILLGFYIHMKKQAVDQSVNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAFWGEVIGTMLLIVFGAGVCAGVNLKKSLSHQSGWIVIVFGWGLGVAMAVYAVGGISG
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCC
AHLNPAVTLGLAFVGDFPWEEVPSYILGQMIGAFLGAVLVFLHYLPHWKETEDQGAKLGV
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FSTGPAIPNTFANLFSETLGTFILVLGLLTIGANKFTDGLNPLVVGFLIVAIGISLGGTT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFVLPIAGKGSSNWKYAWIPVLGPALGGSFAGVFYNAVFKGHITN
CCEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHCCCCH
TFWIVSVILVVILLGFYIHMKKQAVDQSVNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]