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The map label for this gene is kinE [H]

Identifier: 52784753

GI number: 52784753

Start: 979205

End: 981229

Strand: Direct

Name: kinE [H]

Synonym: BLi00981

Alternate gene names: 52784753

Gene position: 979205-981229 (Clockwise)

Preceding gene: 52784751

Following gene: 52784754

Centisome position: 23.19

GC content: 43.85

Gene sequence:

>2025_bases
ATGCTGGGCTCAAGTGAGACGGTTGGAAAAAAGTCAGGTTTATTTGGTAATGATTCTGTGTATCCAAAGGTGCAAACAGA
AGTATATAAAAAAATCAATGAACTGAGTGGTTTTCATAGGCTGGATGATATTTATCTTTCCTTGATCGACAAGTATATGT
TTCCTTGTCTGATCCTGAATGACCGGGGAGAAATTGTCGCGTCCTCGGAAAGTGCTGATCAATATTTGAAAAGCTCAAAA
GGCAGCATTCATCAGGTTTTTCCCGCGAGCTTATCCGTACAGGTCAATGCCGCGTTACAGCGGGCGAAGAAAGAGAAAAA
AGAAGTATCGTTTAAAAACGTCCCGATTTATTTAAGCGGAGAACTATCCTTTGTTCATTTGAGATTAAAATGTTTCTTCC
GCCAGTATCACAAGCTTTATGTGCTGTTTATCGAAAAAGCACAAGAAGGGGCAGCCAACGCGCCGGTATCCGGCGGAGAG
GACGGCCAGCTTCATCTGCAACATCTGTACCGGACGGTAAAAGAGCTCGAAGCGTCCAACGATGCTTTAGTGTCCGCAAA
TCAAAAGCTGCAGGAGGAAAACAGAAAATTGACGGCGCTCAACAGGGCGTTTGAAAAGAAAGTGAACGAGCTGAACACCC
TTAAAAATGATATGAACAATCTGCTGACCAGCACGAACATCGCCGCAGTTTTTTTGGATCAGGAATTGAAATTGAAATTA
TTTACTCCAGAAGCCGCCAATATGTTCGACCTGAAAAACGAGGACCTTTCAAGACCTGCCGATAAGCTGACAGAGAGGTT
TAAGTATGAAGGGTATGCCGATGACTTAAGGCGCACGCTATATGAAAACAAGACGGTCGTAAAAGAGTTTGAAATGCCAG
ACGGCGGCTGGTATGCCGTAAAAATGATCCCTTATTATACATCGGGAAACATGGTTGAAGGCATTGTTGTCACATTCACG
AATGTCACTGAGCTGAAAATGACAAACGAGGCGCTGCATATCAGCTCACAAGCTATAGATCAAAGTCCGGCCAACATATT
GATCACTTCGGTCGACGGTTCGGTCACTTATGCAAACAAACGCTTTTGCCGGATGTTCGGAAAACATCAGGATGACATCA
TCGGCGGCAATGTGTTTGATATTTACCGCAAGCATTTTCACTGTGAGGATCTCACTCGCTTCTGGAAACGCGTCCTTGAA
AAGAAACCATGGTCAGGAGAACTTAATTACCAGGATGAGGAGCAGCAGGACAGGTGGGAGCAAATCTCCCTTGTTCCTGT
TGAGGACAGCGCGGGGAACGTTCAGCAGGTTATGCTGATATCCGAGGACATTACAAACCAAAAGCGATCTGAAAAAATGC
TCATGAAATCGGAAATGCTGTCGGCGGTCGGCCAGCTAGCTGCCGGAATCGCTCATGAAATACGGAACCCGCTCACTTCT
CTGAAAGGGTTTCTCCAGCTGATGATCCAATCCAAAAAATATCAAAAAGACTATGCGGAAGTGATGATGTCCGAATTCAT
CAGGCTTGAAGCAATCATCAACGAATTTCTCGTATTGGCCAAAACGAAATCGAATACATTTGATCCGGTTCAGGTCAACT
CGATTGTTGAAGATGTCTGCATGGTATTGGAGTCGCAGGCCGTGCTGAACAACGTAAGGATCGAGAAACAGCTGAGCGGA
GATCTACCTGAGATTCTCGCTGTTTCAAATGAGCTGAAACAGGTTTTCATCAACATATTAAAAAATGCAATCGAGGCGAT
GGAGGATGCTGAAGGCTTTATTACGATACGTTCCTATTTTGAAAAAGACAGCGTATTCATCACCTTTGAAGATCAGGGAA
AGGGCATCTCAAAAGAAGTGCTAGAAAAGCTTGGGGAGCCATTTTATACAACGAAGGAAAAAGGAACCGGTCTCGGATTA
ATGGTTACATTTAAAATTATTGAAAATCACGGCGGAAGCATTCATTTCGAAAGCGAAGAAGGAAAAGGAACGATCGTAAA
ATTGAAGCTTCCGATTAAAGAATGA

Upstream 100 bases:

>100_bases
GATTCTCCCTCCGTAAACGATTTCAGCTGCCAATCATCATGAAATGCAAAAAAGGTGTTTTCCTTTTTGATTTTGCACTT
AGAAAAGGAGGAGTTTTATA

Downstream 100 bases:

>100_bases
GAATGCGGTCATCCCTATTCTCATTCTTTTTTGAAATTTTTACAAAAAAATCATATAGCCTTTATAATCATCAGATAAAT
TTGACAGGAAGCAAAAAACG

Product: hypothetical protein

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MLGSSETVGKKSGLFGNDSVYPKVQTEVYKKINELSGFHRLDDIYLSLIDKYMFPCLILNDRGEIVASSESADQYLKSSK
GSIHQVFPASLSVQVNAALQRAKKEKKEVSFKNVPIYLSGELSFVHLRLKCFFRQYHKLYVLFIEKAQEGAANAPVSGGE
DGQLHLQHLYRTVKELEASNDALVSANQKLQEENRKLTALNRAFEKKVNELNTLKNDMNNLLTSTNIAAVFLDQELKLKL
FTPEAANMFDLKNEDLSRPADKLTERFKYEGYADDLRRTLYENKTVVKEFEMPDGGWYAVKMIPYYTSGNMVEGIVVTFT
NVTELKMTNEALHISSQAIDQSPANILITSVDGSVTYANKRFCRMFGKHQDDIIGGNVFDIYRKHFHCEDLTRFWKRVLE
KKPWSGELNYQDEEQQDRWEQISLVPVEDSAGNVQQVMLISEDITNQKRSEKMLMKSEMLSAVGQLAAGIAHEIRNPLTS
LKGFLQLMIQSKKYQKDYAEVMMSEFIRLEAIINEFLVLAKTKSNTFDPVQVNSIVEDVCMVLESQAVLNNVRIEKQLSG
DLPEILAVSNELKQVFINILKNAIEAMEDAEGFITIRSYFEKDSVFITFEDQGKGISKEVLEKLGEPFYTTKEKGTGLGL
MVTFKIIENHGGSIHFESEEGKGTIVKLKLPIKE

Sequences:

>Translated_674_residues
MLGSSETVGKKSGLFGNDSVYPKVQTEVYKKINELSGFHRLDDIYLSLIDKYMFPCLILNDRGEIVASSESADQYLKSSK
GSIHQVFPASLSVQVNAALQRAKKEKKEVSFKNVPIYLSGELSFVHLRLKCFFRQYHKLYVLFIEKAQEGAANAPVSGGE
DGQLHLQHLYRTVKELEASNDALVSANQKLQEENRKLTALNRAFEKKVNELNTLKNDMNNLLTSTNIAAVFLDQELKLKL
FTPEAANMFDLKNEDLSRPADKLTERFKYEGYADDLRRTLYENKTVVKEFEMPDGGWYAVKMIPYYTSGNMVEGIVVTFT
NVTELKMTNEALHISSQAIDQSPANILITSVDGSVTYANKRFCRMFGKHQDDIIGGNVFDIYRKHFHCEDLTRFWKRVLE
KKPWSGELNYQDEEQQDRWEQISLVPVEDSAGNVQQVMLISEDITNQKRSEKMLMKSEMLSAVGQLAAGIAHEIRNPLTS
LKGFLQLMIQSKKYQKDYAEVMMSEFIRLEAIINEFLVLAKTKSNTFDPVQVNSIVEDVCMVLESQAVLNNVRIEKQLSG
DLPEILAVSNELKQVFINILKNAIEAMEDAEGFITIRSYFEKDSVFITFEDQGKGISKEVLEKLGEPFYTTKEKGTGLGL
MVTFKIIENHGGSIHFESEEGKGTIVKLKLPIKE
>Mature_674_residues
MLGSSETVGKKSGLFGNDSVYPKVQTEVYKKINELSGFHRLDDIYLSLIDKYMFPCLILNDRGEIVASSESADQYLKSSK
GSIHQVFPASLSVQVNAALQRAKKEKKEVSFKNVPIYLSGELSFVHLRLKCFFRQYHKLYVLFIEKAQEGAANAPVSGGE
DGQLHLQHLYRTVKELEASNDALVSANQKLQEENRKLTALNRAFEKKVNELNTLKNDMNNLLTSTNIAAVFLDQELKLKL
FTPEAANMFDLKNEDLSRPADKLTERFKYEGYADDLRRTLYENKTVVKEFEMPDGGWYAVKMIPYYTSGNMVEGIVVTFT
NVTELKMTNEALHISSQAIDQSPANILITSVDGSVTYANKRFCRMFGKHQDDIIGGNVFDIYRKHFHCEDLTRFWKRVLE
KKPWSGELNYQDEEQQDRWEQISLVPVEDSAGNVQQVMLISEDITNQKRSEKMLMKSEMLSAVGQLAAGIAHEIRNPLTS
LKGFLQLMIQSKKYQKDYAEVMMSEFIRLEAIINEFLVLAKTKSNTFDPVQVNSIVEDVCMVLESQAVLNNVRIEKQLSG
DLPEILAVSNELKQVFINILKNAIEAMEDAEGFITIRSYFEKDSVFITFEDQGKGISKEVLEKLGEPFYTTKEKGTGLGL
MVTFKIIENHGGSIHFESEEGKGTIVKLKLPIKE

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=262, Percent_Identity=33.206106870229, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=35.9307359307359, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=29.0836653386454, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI48994928, Length=352, Percent_Identity=24.7159090909091, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=25.8064516129032, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788713, Length=277, Percent_Identity=27.0758122743682, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=29.004329004329, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786600, Length=346, Percent_Identity=22.5433526011561, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789149, Length=238, Percent_Identity=24.7899159663866, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788393, Length=218, Percent_Identity=24.7706422018349, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=217, Percent_Identity=26.2672811059908, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=227, Percent_Identity=25.5506607929515, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=278, Percent_Identity=28.0575539568345, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76519; Mature: 76519

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGSSETVGKKSGLFGNDSVYPKVQTEVYKKINELSGFHRLDDIYLSLIDKYMFPCLILN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DRGEIVASSESADQYLKSSKGSIHQVFPASLSVQVNAALQRAKKEKKEVSFKNVPIYLSG
CCCCEEECCCHHHHHHHCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEC
ELSFVHLRLKCFFRQYHKLYVLFIEKAQEGAANAPVSGGEDGQLHLQHLYRTVKELEASN
CEEEEHHHHHHHHHHHHHEEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DALVSANQKLQEENRKLTALNRAFEKKVNELNTLKNDMNNLLTSTNIAAVFLDQELKLKL
CCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
FTPEAANMFDLKNEDLSRPADKLTERFKYEGYADDLRRTLYENKTVVKEFEMPDGGWYAV
ECCCCCCCCCCCCCHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEE
KMIPYYTSGNMVEGIVVTFTNVTELKMTNEALHISSQAIDQSPANILITSVDGSVTYANK
EEEEEECCCCEECEEEEEEECCEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCEEEHHH
RFCRMFGKHQDDIIGGNVFDIYRKHFHCEDLTRFWKRVLEKKPWSGELNYQDEEQQDRWE
HHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
QISLVPVEDSAGNVQQVMLISEDITNQKRSEKMLMKSEMLSAVGQLAAGIAHEIRNPLTS
EEEEEEECCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGFLQLMIQSKKYQKDYAEVMMSEFIRLEAIINEFLVLAKTKSNTFDPVQVNSIVEDVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHH
MVLESQAVLNNVRIEKQLSGDLPEILAVSNELKQVFINILKNAIEAMEDAEGFITIRSYF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
EKDSVFITFEDQGKGISKEVLEKLGEPFYTTKEKGTGLGLMVTFKIIENHGGSIHFESEE
CCCCEEEEECCCCCCCHHHHHHHCCCCCEECCCCCCCCEEEEEEEEECCCCCEEEEECCC
GKGTIVKLKLPIKE
CCEEEEEEEECCCC
>Mature Secondary Structure
MLGSSETVGKKSGLFGNDSVYPKVQTEVYKKINELSGFHRLDDIYLSLIDKYMFPCLILN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DRGEIVASSESADQYLKSSKGSIHQVFPASLSVQVNAALQRAKKEKKEVSFKNVPIYLSG
CCCCEEECCCHHHHHHHCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEC
ELSFVHLRLKCFFRQYHKLYVLFIEKAQEGAANAPVSGGEDGQLHLQHLYRTVKELEASN
CEEEEHHHHHHHHHHHHHEEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DALVSANQKLQEENRKLTALNRAFEKKVNELNTLKNDMNNLLTSTNIAAVFLDQELKLKL
CCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
FTPEAANMFDLKNEDLSRPADKLTERFKYEGYADDLRRTLYENKTVVKEFEMPDGGWYAV
ECCCCCCCCCCCCCHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEE
KMIPYYTSGNMVEGIVVTFTNVTELKMTNEALHISSQAIDQSPANILITSVDGSVTYANK
EEEEEECCCCEECEEEEEEECCEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCEEEHHH
RFCRMFGKHQDDIIGGNVFDIYRKHFHCEDLTRFWKRVLEKKPWSGELNYQDEEQQDRWE
HHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
QISLVPVEDSAGNVQQVMLISEDITNQKRSEKMLMKSEMLSAVGQLAAGIAHEIRNPLTS
EEEEEEECCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGFLQLMIQSKKYQKDYAEVMMSEFIRLEAIINEFLVLAKTKSNTFDPVQVNSIVEDVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHH
MVLESQAVLNNVRIEKQLSGDLPEILAVSNELKQVFINILKNAIEAMEDAEGFITIRSYF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
EKDSVFITFEDQGKGISKEVLEKLGEPFYTTKEKGTGLGLMVTFKIIENHGGSIHFESEE
CCCCEEEEECCCCCCCHHHHHHHCCCCCEECCCCCCCCEEEEEEEEECCCCCEEEEECCC
GKGTIVKLKLPIKE
CCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]