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The map label for this gene is yflM [H]

Identifier: 52784584

GI number: 52784584

Start: 797308

End: 798405

Strand: Direct

Name: yflM [H]

Synonym: BLi00785

Alternate gene names: 52784584

Gene position: 797308-798405 (Clockwise)

Preceding gene: 52784583

Following gene: 52784588

Centisome position: 18.88

GC content: 49.27

Gene sequence:

>1098_bases
TTGGAATCACATGAAGAATTATGGAGGGAAGCCAAGGCCTTCATCGAGCTCTGCTACGGGGAACTGTCGAAGTCCGAAGA
AGAAACAAGGATGCGCTTACATAAAATAGATAAAGAAATCAGAGAAACCGGAAGCTATACACATACATTAGAAGAAATCG
AACATGGAGCCAGAATGGCGTGGAGAAACAGCAGCCGCTGCATCGGCAGGCTGTTTTGGCACTCTCTTACTGTCATCGAT
CAAAGAGGCGTTCAAACCGAGGCAGAGGTGCGGGATGCGCTTTTCCACCATATTCAGCTTGCAACAAACGGAGGGAAAAT
CAGACCGTTCATTACGGTTTTCCCCCCGGAACAAAACGGACAAAAAAAAGTGTCCATTTGGAACCACCAGCTGATCAGGT
ATGCGGGGTATGAAACTGAGCAAGGCGTTATCGGCGATCCGGCGTCATTGGAACTGACAAAGGCGTGTGAAGCGCTCGGC
TGGAAAGGCGAAGGTACGCCTTTTGACGTATTGCCGCTTGTTTTTCAAATAGATGGGCGTCCTCCTGTGTGGTATGAGCT
GCCTCGTGAAATCGTCAAAGAGGTTCGGATTGAACATCCGGAGATTGAAGGCTTCAAAAGTTTGGGGCTGAAGTGGTACG
CTGTACCGATTATAGCCGATATGAAGCTGGAAGTCGGAGGGATTCATTATAATGCCGCCCCGTTTAACGGCTGGTATATG
GGAACTGAAATCGGAGCGCGCAACCTCGCGGATGCAGACCGCTATAATGCGCTCGAAAAAGTGGCAGCCATTATGGGGCT
TGATACGGAGCGGAATACTTCGCTGTGGAAAGACAAGGCGCTTGTTGAATTAAATACTGCGGTGCTTCACTCCTATAAAA
AAGCCGGCGTCAGCATCGTCGACCACCATACTGCGGCAAGCCAATTCAAGCGGTTCGAGGAGCAGGAGGCTGAAGCCGGA
AGGGAGCTTGCAGCAAGCTGGACGTGGCTGATTCCGCCGTTATCGCCGGCAGCGACCCATATCTTTCACAGCTATTATGA
AAATAAAACGCTGAAACCGAATTACTTTTATCAGGAAAAGCCTTACCTTAACAAATAA

Upstream 100 bases:

>100_bases
TTTTTAGCGGTTTTTTGTTTTTTCATACATAATTGTTTTAAACTGAGATCGAAACCTATACAATAAATATCAGTCTGAAA
TCTGGAAGGAGAGAATCCGG

Downstream 100 bases:

>100_bases
AATCCTCAGGAAAAGCGGCTACCCAACAATGCCGCCTTTTCTGAGGAGAAGCTAAAAGGCTCCAAATCGGAGCCTTTTTG
AATCGTCTCAGCTTTTCATA

Product: YflM

Products: NA

Alternate protein names: NOSoxy-like protein [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MESHEELWREAKAFIELCYGELSKSEEETRMRLHKIDKEIRETGSYTHTLEEIEHGARMAWRNSSRCIGRLFWHSLTVID
QRGVQTEAEVRDALFHHIQLATNGGKIRPFITVFPPEQNGQKKVSIWNHQLIRYAGYETEQGVIGDPASLELTKACEALG
WKGEGTPFDVLPLVFQIDGRPPVWYELPREIVKEVRIEHPEIEGFKSLGLKWYAVPIIADMKLEVGGIHYNAAPFNGWYM
GTEIGARNLADADRYNALEKVAAIMGLDTERNTSLWKDKALVELNTAVLHSYKKAGVSIVDHHTAASQFKRFEEQEAEAG
RELAASWTWLIPPLSPAATHIFHSYYENKTLKPNYFYQEKPYLNK

Sequences:

>Translated_365_residues
MESHEELWREAKAFIELCYGELSKSEEETRMRLHKIDKEIRETGSYTHTLEEIEHGARMAWRNSSRCIGRLFWHSLTVID
QRGVQTEAEVRDALFHHIQLATNGGKIRPFITVFPPEQNGQKKVSIWNHQLIRYAGYETEQGVIGDPASLELTKACEALG
WKGEGTPFDVLPLVFQIDGRPPVWYELPREIVKEVRIEHPEIEGFKSLGLKWYAVPIIADMKLEVGGIHYNAAPFNGWYM
GTEIGARNLADADRYNALEKVAAIMGLDTERNTSLWKDKALVELNTAVLHSYKKAGVSIVDHHTAASQFKRFEEQEAEAG
RELAASWTWLIPPLSPAATHIFHSYYENKTLKPNYFYQEKPYLNK
>Mature_365_residues
MESHEELWREAKAFIELCYGELSKSEEETRMRLHKIDKEIRETGSYTHTLEEIEHGARMAWRNSSRCIGRLFWHSLTVID
QRGVQTEAEVRDALFHHIQLATNGGKIRPFITVFPPEQNGQKKVSIWNHQLIRYAGYETEQGVIGDPASLELTKACEALG
WKGEGTPFDVLPLVFQIDGRPPVWYELPREIVKEVRIEHPEIEGFKSLGLKWYAVPIIADMKLEVGGIHYNAAPFNGWYM
GTEIGARNLADADRYNALEKVAAIMGLDTERNTSLWKDKALVELNTAVLHSYKKAGVSIVDHHTAASQFKRFEEQEAEAG
RELAASWTWLIPPLSPAATHIFHSYYENKTLKPNYFYQEKPYLNK

Specific function: Catalyzes the production of nitric oxide [H]

COG id: COG4362

COG function: function code PE; Nitric oxide synthase, oxygenase domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NOS family. Bacterial NOS oxygenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=356, Percent_Identity=46.6292134831461, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI231571356, Length=360, Percent_Identity=45.8333333333333, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI231571314, Length=360, Percent_Identity=45.8333333333333, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI40254422, Length=360, Percent_Identity=45.8333333333333, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI231571329, Length=360, Percent_Identity=45.8333333333333, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI10835173, Length=359, Percent_Identity=44.0111420612813, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI78706866, Length=353, Percent_Identity=46.1756373937677, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI78706868, Length=353, Percent_Identity=46.1756373937677, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI78706882, Length=353, Percent_Identity=46.1756373937677, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24583543, Length=353, Percent_Identity=46.1756373937677, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI78706876, Length=353, Percent_Identity=46.1756373937677, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI78706870, Length=353, Percent_Identity=46.1756373937677, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI78706878, Length=353, Percent_Identity=46.1756373937677, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI78706880, Length=353, Percent_Identity=46.1756373937677, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI78706872, Length=177, Percent_Identity=38.4180790960452, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017142
- InterPro:   IPR004030 [H]

Pfam domain/function: PF02898 NO_synthase [H]

EC number: NA

Molecular weight: Translated: 41853; Mature: 41853

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS60001 NOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESHEELWREAKAFIELCYGELSKSEEETRMRLHKIDKEIRETGSYTHTLEEIEHGARMA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WRNSSRCIGRLFWHSLTVIDQRGVQTEAEVRDALFHHIQLATNGGKIRPFITVFPPEQNG
HCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCC
QKKVSIWNHQLIRYAGYETEQGVIGDPASLELTKACEALGWKGEGTPFDVLPLVFQIDGR
CEEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEECCC
PPVWYELPREIVKEVRIEHPEIEGFKSLGLKWYAVPIIADMKLEVGGIHYNAAPFNGWYM
CCHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEEEEEEEEEEECCEEECCCCCCCEEE
GTEIGARNLADADRYNALEKVAAIMGLDTERNTSLWKDKALVELNTAVLHSYKKAGVSIV
CCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCEEE
DHHTAASQFKRFEEQEAEAGRELAASWTWLIPPLSPAATHIFHSYYENKTLKPNYFYQEK
EHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCEECCC
PYLNK
CCCCC
>Mature Secondary Structure
MESHEELWREAKAFIELCYGELSKSEEETRMRLHKIDKEIRETGSYTHTLEEIEHGARMA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WRNSSRCIGRLFWHSLTVIDQRGVQTEAEVRDALFHHIQLATNGGKIRPFITVFPPEQNG
HCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCC
QKKVSIWNHQLIRYAGYETEQGVIGDPASLELTKACEALGWKGEGTPFDVLPLVFQIDGR
CEEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEECCC
PPVWYELPREIVKEVRIEHPEIEGFKSLGLKWYAVPIIADMKLEVGGIHYNAAPFNGWYM
CCHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEEEEEEEEEEECCEEECCCCCCCEEE
GTEIGARNLADADRYNALEKVAAIMGLDTERNTSLWKDKALVELNTAVLHSYKKAGVSIV
CCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCEEE
DHHTAASQFKRFEEQEAEAGRELAASWTWLIPPLSPAATHIFHSYYENKTLKPNYFYQEK
EHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCEECCC
PYLNK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 11856757; 12220171 [H]