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The map label for this gene is yacA

Identifier: 52783924

GI number: 52783924

Start: 79524

End: 80954

Strand: Direct

Name: yacA

Synonym: BLi00083

Alternate gene names: 52783924

Gene position: 79524-80954 (Clockwise)

Preceding gene: 52783923

Following gene: 52783925

Centisome position: 1.88

GC content: 45.07

Gene sequence:

>1431_bases
GTGAAAAGTATTGAAGATTTCATGAAAAAATACGAACTGTCATTTGATGGAGTGACGCTGATTGCAGGTGTCTCCGGCGG
ACCTGATTCGATGGCGCTTTTGCATGCTCTGCACCATACGGTTCCCGCTTCTGCAACGCTGATTGCCGCACACGTCGATC
ATATGTTCAGAGGCGAAGAGTCCGAGCGTGATATGCGGTTCGTTCAGGACTACTGCGAGGCGGAAGGCATACAATGCGAG
GCGGTGCAAATCGATGTCCTGGCATTTGCCGCTGAAAACAATCTGAATAAACAGGCTGCTGCGAGAGAATGCCGGTATGC
GTTTTTTCAAGAGTTAATGAAAAGGCACCGGGCCGAATACCTGGTTCTGGGTCATCATGGAGATGATCAGGTGGAGACGA
TGCTGATGAAGATGGCAAAAGGCACTGTCGGAATCGGGCTTGCCGGAATTCAGCCAAAGCGCCGTTTTGACAGCGGCTGG
CTGCTCAGGCCGTTTCTGAAGCTGTCAAAGGATGATCTTTTGGCTTACTGCAAAGAGAATCGAATACCGTTTAGAACCGA
TCCGAGCAACAGTGAGGATGATTACACCAGGAACCGGTTCAGGCACCATGTTCTCCCTTTTCTGAAGAAAGAGTCGGAAG
ATGTCCATAAAAGGTTTCAATCCGTCAGTGAATTCCTGACAGAAGACGAATTATACTTACAGGCATTAACGAAAGACAAA
ATGAATACAGTAATCACAAGCAAGTCAAGTTCAGGCGTGGAAATCAGCATCGAACGACTTCTTGCGCTCCCTATGCCTTT
ACAAAGAAGGGGAGTTCATCTAATATTAAACTATCTTTATGAAAACGTTCCATCAGCGTTTTCAGCCCATCATATTCAAG
TGTTTCTGGATTGGATTTCACAAGACGGACCATCCGGTTCCCTTGATTTTCCGAATGGGTTAAAAGTCGCAAAATCTTAT
CATACATGTTTGTTTACATTTCAGCGTTTACAATGTGAAAATGTTTCCTATCATTATGAAATAAGCGGGGCAGGCGAAGA
AGAACTGATACTTCCCGGCGGCTCCTCCCTTCATGTGAGCGGACCCGGTTCAACCCGGAAGCTGCAAGGAAATGATGTTT
TTGCAACAAGTCCCAAGCAGGTTCAGTTTCCCCTTTACGTACGGACAAGGCAAAACGGAGACCGAGTCAAACTTAAAGGG
ATGAACGGCTCAAAAAAGGTTAAAGATATATTTATTGATGAAAAAGTGCCTCTTTCAAAAAGAGACAGCTGGCCCATTGT
AACAGACGCGGTTGGAAACATTATCTGGATACCTGGTCTGAAAAAAACCATTTTTGAGGAACTTGATGTTACAAACAACG
ATCGCATTGTATTACAATATAGACAGCATGAAAAGTGTAGGGGGCTAGCAAAGAATGAAACAGGATATTGA

Upstream 100 bases:

>100_bases
GTGCATTCTCGGCGGATAGCTCTTCTTTGCAGGGACCGATTGAAAGTGTTAGGATATTTATATCTTTTGAAATACACCCT
GCTCTTAGTGGGAGGACATT

Downstream 100 bases:

>100_bases
AAATATCTTGATCTCGGAAGAAGAGATCCAAAAGAAAGTCAAGGAGCTGGGCGCGACTCTGACAGAAGAGTACAGCGGCA
AGTTTCCGCTGGCAATCGGT

Product: YacA

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKSIEDFMKKYELSFDGVTLIAGVSGGPDSMALLHALHHTVPASATLIAAHVDHMFRGEESERDMRFVQDYCEAEGIQCE
AVQIDVLAFAAENNLNKQAAARECRYAFFQELMKRHRAEYLVLGHHGDDQVETMLMKMAKGTVGIGLAGIQPKRRFDSGW
LLRPFLKLSKDDLLAYCKENRIPFRTDPSNSEDDYTRNRFRHHVLPFLKKESEDVHKRFQSVSEFLTEDELYLQALTKDK
MNTVITSKSSSGVEISIERLLALPMPLQRRGVHLILNYLYENVPSAFSAHHIQVFLDWISQDGPSGSLDFPNGLKVAKSY
HTCLFTFQRLQCENVSYHYEISGAGEEELILPGGSSLHVSGPGSTRKLQGNDVFATSPKQVQFPLYVRTRQNGDRVKLKG
MNGSKKVKDIFIDEKVPLSKRDSWPIVTDAVGNIIWIPGLKKTIFEELDVTNNDRIVLQYRQHEKCRGLAKNETGY

Sequences:

>Translated_476_residues
MKSIEDFMKKYELSFDGVTLIAGVSGGPDSMALLHALHHTVPASATLIAAHVDHMFRGEESERDMRFVQDYCEAEGIQCE
AVQIDVLAFAAENNLNKQAAARECRYAFFQELMKRHRAEYLVLGHHGDDQVETMLMKMAKGTVGIGLAGIQPKRRFDSGW
LLRPFLKLSKDDLLAYCKENRIPFRTDPSNSEDDYTRNRFRHHVLPFLKKESEDVHKRFQSVSEFLTEDELYLQALTKDK
MNTVITSKSSSGVEISIERLLALPMPLQRRGVHLILNYLYENVPSAFSAHHIQVFLDWISQDGPSGSLDFPNGLKVAKSY
HTCLFTFQRLQCENVSYHYEISGAGEEELILPGGSSLHVSGPGSTRKLQGNDVFATSPKQVQFPLYVRTRQNGDRVKLKG
MNGSKKVKDIFIDEKVPLSKRDSWPIVTDAVGNIIWIPGLKKTIFEELDVTNNDRIVLQYRQHEKCRGLAKNETGY
>Mature_476_residues
MKSIEDFMKKYELSFDGVTLIAGVSGGPDSMALLHALHHTVPASATLIAAHVDHMFRGEESERDMRFVQDYCEAEGIQCE
AVQIDVLAFAAENNLNKQAAARECRYAFFQELMKRHRAEYLVLGHHGDDQVETMLMKMAKGTVGIGLAGIQPKRRFDSGW
LLRPFLKLSKDDLLAYCKENRIPFRTDPSNSEDDYTRNRFRHHVLPFLKKESEDVHKRFQSVSEFLTEDELYLQALTKDK
MNTVITSKSSSGVEISIERLLALPMPLQRRGVHLILNYLYENVPSAFSAHHIQVFLDWISQDGPSGSLDFPNGLKVAKSY
HTCLFTFQRLQCENVSYHYEISGAGEEELILPGGSSLHVSGPGSTRKLQGNDVFATSPKQVQFPLYVRTRQNGDRVKLKG
MNGSKKVKDIFIDEKVPLSKRDSWPIVTDAVGNIIWIPGLKKTIFEELDVTNNDRIVLQYRQHEKCRGLAKNETGY

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=416, Percent_Identity=25.2403846153846, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_BACLD (Q65PF4)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077353.1
- RefSeq:   YP_089753.1
- ProteinModelPortal:   Q65PF4
- SMR:   Q65PF4
- STRING:   Q65PF4
- EnsemblBacteria:   EBBACT00000058193
- EnsemblBacteria:   EBBACT00000059147
- GeneID:   3029360
- GeneID:   3100897
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00083
- KEGG:   bli:BL00503
- NMPDR:   fig|279010.5.peg.1463
- eggNOG:   COG0037
- GeneTree:   EBGT00050000002021
- HOGENOM:   HBG668702
- OMA:   HANDQVE
- ProtClustDB:   CLSK886565
- BioCyc:   BLIC279010-1:BLI00083-MONOMER
- BioCyc:   BLIC279010:BL00503-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 53986; Mature: 53986

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIEDFMKKYELSFDGVTLIAGVSGGPDSMALLHALHHTVPASATLIAAHVDHMFRGEE
CCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
SERDMRFVQDYCEAEGIQCEAVQIDVLAFAAENNLNKQAAARECRYAFFQELMKRHRAEY
CHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
LVLGHHGDDQVETMLMKMAKGTVGIGLAGIQPKRRFDSGWLLRPFLKLSKDDLLAYCKEN
EEEECCCCHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCEEHHHHHCCHHHHHHHHHCC
RIPFRTDPSNSEDDYTRNRFRHHVLPFLKKESEDVHKRFQSVSEFLTEDELYLQALTKDK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MNTVITSKSSSGVEISIERLLALPMPLQRRGVHLILNYLYENVPSAFSAHHIQVFLDWIS
HHHEEECCCCCCCEEEEHHHHHCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QDGPSGSLDFPNGLKVAKSYHTCLFTFQRLQCENVSYHYEISGAGEEELILPGGSSLHVS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCEEEEE
GPGSTRKLQGNDVFATSPKQVQFPLYVRTRQNGDRVKLKGMNGSKKVKDIFIDEKVPLSK
CCCCCCEECCCCEEECCCCCEEEEEEEEECCCCCEEEEECCCCCCCHHEEEECCCCCCCC
RDSWPIVTDAVGNIIWIPGLKKTIFEELDVTNNDRIVLQYRQHEKCRGLAKNETGY
CCCCCEEHHCCCCEEECCCCHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCCCC
>Mature Secondary Structure
MKSIEDFMKKYELSFDGVTLIAGVSGGPDSMALLHALHHTVPASATLIAAHVDHMFRGEE
CCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
SERDMRFVQDYCEAEGIQCEAVQIDVLAFAAENNLNKQAAARECRYAFFQELMKRHRAEY
CHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
LVLGHHGDDQVETMLMKMAKGTVGIGLAGIQPKRRFDSGWLLRPFLKLSKDDLLAYCKEN
EEEECCCCHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCEEHHHHHCCHHHHHHHHHCC
RIPFRTDPSNSEDDYTRNRFRHHVLPFLKKESEDVHKRFQSVSEFLTEDELYLQALTKDK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MNTVITSKSSSGVEISIERLLALPMPLQRRGVHLILNYLYENVPSAFSAHHIQVFLDWIS
HHHEEECCCCCCCEEEEHHHHHCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QDGPSGSLDFPNGLKVAKSYHTCLFTFQRLQCENVSYHYEISGAGEEELILPGGSSLHVS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCEEEEE
GPGSTRKLQGNDVFATSPKQVQFPLYVRTRQNGDRVKLKGMNGSKKVKDIFIDEKVPLSK
CCCCCCEECCCCEEECCCCCEEEEEEEEECCCCCEEEEECCCCCCCHHEEEECCCCCCCC
RDSWPIVTDAVGNIIWIPGLKKTIFEELDVTNNDRIVLQYRQHEKCRGLAKNETGY
CCCCCEEHHCCCCEEECCCCHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA