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The map label for this gene is ipk

Identifier: 52783903

GI number: 52783903

Start: 57962

End: 58831

Strand: Direct

Name: ipk

Synonym: BLi00059

Alternate gene names: 52783903

Gene position: 57962-58831 (Clockwise)

Preceding gene: 52783902

Following gene: 52783904

Centisome position: 1.37

GC content: 48.16

Gene sequence:

>870_bases
TTGCGTATTTTAGAGAAGGCGCCGGCTAAAATTAATTTGTCGCTCGACGTTCGCCACAAACGTCCAGATGGGTATCATGA
AGTAGAGATGGTCATGACGACGATTGATTTGGCGGATCGGGTCGAATTAACAGAGCTGGCAGAAGATAAAGTAACGGTTT
CTTCGCATAACCGCTTTGTTCCCGACGATCAGCGAAACCTTGCTTATCAGGCTGCAATGCTCATGAAAGAACGCTACGGC
ATTAAAAAAGGGGTTTCCATTTTTATTACGAAAGTGATTCCCGTTGCTGCGGGCCTTGCAGGAGGAAGCAGCGATGCGGC
TGCCGTCTTCAGAGGATTGAACCGGCTGTGGGATTTGAAGCTTTCGATGAATGAACTGGCTGAGCTTGGCGCTGAGATCG
GATCTGATGTCTCCTTTTGCGTACATGGAGGAACGGCTCTTGCAACGGGAAGGGGAGAGAAAATCCGTCATATCGAGACT
CCGCCGCACTGCTGGGTAGTGCTGGCAAAGCCGACGATCGGCGTATCAACTGCAGAAGTGTACAAGCAGCTGAAAGTCGA
CGAGATTGAACATCCGGATGTTCAAGGTATGATTGCTGCGATTGAAGAGAAGAATTTTCAAAAAATGTGTGACAAGCTCG
GCAATGTACTCGAATCTGTTACATTGAACATGCACCCCGAAGTCGCGATGATCAAAAATCAAATGAAGCGGTTCGGCGCC
GATGCTGTGCTGATGAGCGGGAGCGGCCCCACTGTGTTCGGACTTGTTCAATACGAATCAAAAGTGCAAAGAATCTATAA
CGGATTAAGAGGGTTTTGCGATCAGGTTTATGCGGTGCGGATGATCGGAGAACAAAATGAACTTGATTAA

Upstream 100 bases:

>100_bases
CGAGGCGTCCTGCCTCAGGCGCATGCATTTGTACGTCCTGTACTTCAAAATATGTTACAATGTGGATACTTATGTTTAGA
TGGAGAAGTAGGTGAATGGA

Downstream 100 bases:

>100_bases
ATCCGTATGTTAAGTTATATTGTTTTTAAAAATATTCGGATTTTGGAGGTAAGTGCATGAAGTTTCGTCGAAGCGGCAGA
TTGGTGGATCTGACAAATTA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRILEKAPAKINLSLDVRHKRPDGYHEVEMVMTTIDLADRVELTELAEDKVTVSSHNRFVPDDQRNLAYQAAMLMKERYG
IKKGVSIFITKVIPVAAGLAGGSSDAAAVFRGLNRLWDLKLSMNELAELGAEIGSDVSFCVHGGTALATGRGEKIRHIET
PPHCWVVLAKPTIGVSTAEVYKQLKVDEIEHPDVQGMIAAIEEKNFQKMCDKLGNVLESVTLNMHPEVAMIKNQMKRFGA
DAVLMSGSGPTVFGLVQYESKVQRIYNGLRGFCDQVYAVRMIGEQNELD

Sequences:

>Translated_289_residues
MRILEKAPAKINLSLDVRHKRPDGYHEVEMVMTTIDLADRVELTELAEDKVTVSSHNRFVPDDQRNLAYQAAMLMKERYG
IKKGVSIFITKVIPVAAGLAGGSSDAAAVFRGLNRLWDLKLSMNELAELGAEIGSDVSFCVHGGTALATGRGEKIRHIET
PPHCWVVLAKPTIGVSTAEVYKQLKVDEIEHPDVQGMIAAIEEKNFQKMCDKLGNVLESVTLNMHPEVAMIKNQMKRFGA
DAVLMSGSGPTVFGLVQYESKVQRIYNGLRGFCDQVYAVRMIGEQNELD
>Mature_289_residues
MRILEKAPAKINLSLDVRHKRPDGYHEVEMVMTTIDLADRVELTELAEDKVTVSSHNRFVPDDQRNLAYQAAMLMKERYG
IKKGVSIFITKVIPVAAGLAGGSSDAAAVFRGLNRLWDLKLSMNELAELGAEIGSDVSFCVHGGTALATGRGEKIRHIET
PPHCWVVLAKPTIGVSTAEVYKQLKVDEIEHPDVQGMIAAIEEKNFQKMCDKLGNVLESVTLNMHPEVAMIKNQMKRFGA
DAVLMSGSGPTVFGLVQYESKVQRIYNGLRGFCDQVYAVRMIGEQNELD

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=271, Percent_Identity=29.520295202952, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_BACLD (Q65PH5)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077332.2
- RefSeq:   YP_089732.1
- ProteinModelPortal:   Q65PH5
- SMR:   Q65PH5
- STRING:   Q65PH5
- EnsemblBacteria:   EBBACT00000057108
- EnsemblBacteria:   EBBACT00000059005
- GeneID:   3027307
- GeneID:   3101513
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00059
- KEGG:   bli:BL00525
- NMPDR:   fig|279010.5.peg.1341
- eggNOG:   COG1947
- GeneTree:   EBGT00050000000875
- HOGENOM:   HBG734593
- ProtClustDB:   PRK00128
- BioCyc:   BLIC279010-1:BLI00059-MONOMER
- BioCyc:   BLIC279010:BL00525-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31943; Mature: 31943

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILEKAPAKINLSLDVRHKRPDGYHEVEMVMTTIDLADRVELTELAEDKVTVSSHNRFV
CCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
PDDQRNLAYQAAMLMKERYGIKKGVSIFITKVIPVAAGLAGGSSDAAAVFRGLNRLWDLK
CCCHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
LSMNELAELGAEIGSDVSFCVHGGTALATGRGEKIRHIETPPHCWVVLAKPTIGVSTAEV
CCHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCEEECCCCCCEEEEEECCCCCCCHHHH
YKQLKVDEIEHPDVQGMIAAIEEKNFQKMCDKLGNVLESVTLNMHPEVAMIKNQMKRFGA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
DAVLMSGSGPTVFGLVQYESKVQRIYNGLRGFCDQVYAVRMIGEQNELD
CEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRILEKAPAKINLSLDVRHKRPDGYHEVEMVMTTIDLADRVELTELAEDKVTVSSHNRFV
CCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
PDDQRNLAYQAAMLMKERYGIKKGVSIFITKVIPVAAGLAGGSSDAAAVFRGLNRLWDLK
CCCHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
LSMNELAELGAEIGSDVSFCVHGGTALATGRGEKIRHIETPPHCWVVLAKPTIGVSTAEV
CCHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCEEECCCCCCEEEEEECCCCCCCHHHH
YKQLKVDEIEHPDVQGMIAAIEEKNFQKMCDKLGNVLESVTLNMHPEVAMIKNQMKRFGA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
DAVLMSGSGPTVFGLVQYESKVQRIYNGLRGFCDQVYAVRMIGEQNELD
CEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA