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The map label for this gene is recF

Identifier: 52783859

GI number: 52783859

Start: 3364

End: 4476

Strand: Direct

Name: recF

Synonym: BLi00004

Alternate gene names: 52783859

Gene position: 3364-4476 (Clockwise)

Preceding gene: 52783858

Following gene: 52783860

Centisome position: 0.08

GC content: 42.77

Gene sequence:

>1113_bases
TTGTATATCCAAAATCTTACATTATCGTCTTACCGTAATTATGAGCGCCTTGACCTTCAATTTGAGAACAAAGTGAACGT
GATTATCGGAGAGAACGCTCAAGGAAAAACAAATTTGATGGAAGCGATCTATGTGCTTGCGATGGCGAAATCCCATCGGA
CGTCAAATGATAAAGAACTCATACGATGGGATGAAGACTATGCTAAAATAGAAGGCAGGGTCATTAAAAAAAACGGTTCT
GTTCCCATTCAGCTTGTGATTTCCAAAAAAGGAAAAAAAGGTAAAGTGAATCATATTGAACAGCAGAAGCTGAGCCAGTA
TGTCGGGGCTGTCAACACGATTATGTTCGCGCCGGAGGATTTAAATCTCGTAAAAGGAAGTCCTCAGGTCAGAAGAAGGT
TTCTCGACATGGAAATCGGCCAAGTATCACCTGTCTATCTTCATGACCTTTCTCTTTACCAAAAAATCCTTTCACAACGG
AATCATTTCTTGAAGCAGCTTCAAACGAGAAAGCAAACCGATCAAACGATGCTTGACGTGCTGACGGAACAGCTTACGGA
ATTCGCGGCAAAGGTTGTGATGAAACGGCTTCAGTTTGTCGATCAGCTTGAAAAATGGGCTCAGCCCATTCATTCCGGAA
TTTCAAGAGGTCTGGAAGAGCTGACGTTAAAGTATCATACGTCTCTTCACGTATCAGATTCGCCCGATTTGTCGAAAATG
ATCAATAGTTATCAAGAAACGTTTTCTAAATTAAGAGATAAAGAAATAGAACGGGGGGTATCTCTTTCAGGTCCCCACAG
GGATGATGTTCTTTTCTATGTCAATGGCCGTGATGTGCAGACTTACGGATCGCAGGGCCAGCAGCGCACGACTGCATTGT
CGCTTAAATTGGCTGAAATCGACTTGATTCAGGAAGAGATAGGAGAATACCCGATTCTGCTTTTGGATGATGTTTTATCA
GAGCTGGACGACTATCGGCAGTCTCACTTGCTCCACACCATTCAAGGCCGCGTTCAAACATTCGTAACAACGACAAGCGT
TGATGGAATTGATCATAAAACCTTAAACGAAGCGGAAATCTTTCGTGTTGAAAATGGCACGCTATCGGACTGA

Upstream 100 bases:

>100_bases
CCGGATAACCGCCGCGGACGAAAACTGTATGTCGGAGATGTGGTGACGATTGAAGGATTCGGTTCATTTCAAGTCGTCGG
CTAAAAGCAGGTGGTATTGA

Downstream 100 bases:

>100_bases
AAAAATGGGGTGAGGGATTGTATATCCACTTAGGTGACGATTTTGTCGTCTCAACGCGTGAAATTGTGGCTATTTTTGAT
TACAAGGCAAAGACATCGCC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MYIQNLTLSSYRNYERLDLQFENKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDKELIRWDEDYAKIEGRVIKKNGS
VPIQLVISKKGKKGKVNHIEQQKLSQYVGAVNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDQTMLDVLTEQLTEFAAKVVMKRLQFVDQLEKWAQPIHSGISRGLEELTLKYHTSLHVSDSPDLSKM
INSYQETFSKLRDKEIERGVSLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIQEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHKTLNEAEIFRVENGTLSD

Sequences:

>Translated_370_residues
MYIQNLTLSSYRNYERLDLQFENKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDKELIRWDEDYAKIEGRVIKKNGS
VPIQLVISKKGKKGKVNHIEQQKLSQYVGAVNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDQTMLDVLTEQLTEFAAKVVMKRLQFVDQLEKWAQPIHSGISRGLEELTLKYHTSLHVSDSPDLSKM
INSYQETFSKLRDKEIERGVSLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIQEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHKTLNEAEIFRVENGTLSD
>Mature_370_residues
MYIQNLTLSSYRNYERLDLQFENKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDKELIRWDEDYAKIEGRVIKKNGS
VPIQLVISKKGKKGKVNHIEQQKLSQYVGAVNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDQTMLDVLTEQLTEFAAKVVMKRLQFVDQLEKWAQPIHSGISRGLEELTLKYHTSLHVSDSPDLSKM
INSYQETFSKLRDKEIERGVSLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIQEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHKTLNEAEIFRVENGTLSD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=382, Percent_Identity=25.3926701570681, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_BACLD (Q65PL9)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077286.1
- RefSeq:   YP_089688.1
- ProteinModelPortal:   Q65PL9
- STRING:   Q65PL9
- EnsemblBacteria:   EBBACT00000055572
- EnsemblBacteria:   EBBACT00000059113
- GeneID:   3029476
- GeneID:   3101230
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00004
- KEGG:   bli:BL00079
- NMPDR:   fig|279010.5.peg.1194
- eggNOG:   COG1195
- GeneTree:   EBGT00050000002258
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   BLIC279010-1:BLI00004-MONOMER
- BioCyc:   BLIC279010:BL00079-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42483; Mature: 42483

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIQNLTLSSYRNYERLDLQFENKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDKEL
CEEECCCHHHCCCCHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
IRWDEDYAKIEGRVIKKNGSVPIQLVISKKGKKGKVNHIEQQKLSQYVGAVNTIMFAPED
HEECHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEECCCC
LNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQRNHFLKQLQTRKQTDQTMLDV
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTEQLTEFAAKVVMKRLQFVDQLEKWAQPIHSGISRGLEELTLKYHTSLHVSDSPDLSKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCHHHH
INSYQETFSKLRDKEIERGVSLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEHHCCCCCHHHHHHEEEHHHH
DLIQEEIGEYPILLLDDVLSELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHKTLNEAEI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
FRVENGTLSD
EEECCCCCCC
>Mature Secondary Structure
MYIQNLTLSSYRNYERLDLQFENKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDKEL
CEEECCCHHHCCCCHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
IRWDEDYAKIEGRVIKKNGSVPIQLVISKKGKKGKVNHIEQQKLSQYVGAVNTIMFAPED
HEECHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEECCCC
LNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQRNHFLKQLQTRKQTDQTMLDV
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTEQLTEFAAKVVMKRLQFVDQLEKWAQPIHSGISRGLEELTLKYHTSLHVSDSPDLSKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCHHHH
INSYQETFSKLRDKEIERGVSLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEHHCCCCCHHHHHHEEEHHHH
DLIQEEIGEYPILLLDDVLSELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHKTLNEAEI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
FRVENGTLSD
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA