Click here to switch to the map view.

The map label for this gene is gidB [H]

Identifier: 52145290

GI number: 52145290

Start: 5294486

End: 5295205

Strand: Reverse

Name: gidB [H]

Synonym: BCZK5179

Alternate gene names: 52145290

Gene position: 5295205-5294486 (Counterclockwise)

Preceding gene: 52145291

Following gene: 52145289

Centisome position: 99.89

GC content: 36.39

Gene sequence:

>720_bases
ATGAACATAGAACAATTTCAATCTATGCTAGAAGAGAAGGGTATCACCCTCTCTTCTAGACAATTAGAGCAGTTCGAAAT
CTACTTCGAAACATTAGTAGAATGGAATGAAAAAATGAATTTAACGGCTATTACGGAGAAAGAGGAAGTATATTTAAAAC
ACTTTTTTGATTCCATTACAGCTGCTTTTTATTATGATTTTTCAAAACCATTCTCAATTTGTGATGTTGGCGCAGGAGCT
GGATTCCCAAGTATTCCTTTAAAAATTTGTTTCCCGCATTTAAAAGTGACAATTGTAGACTCATTGCAAAAACGTATTAA
TTTCTTAAATCATTTAGCACAAAAGTTGGAATTAAGTGACGTCGCGTTTTGTCACGATCGTGCTGAAACATTTGGGAAAA
AAGAAGGTGTACGTGAAGCGTACGACATTGTAATGGCACGTGCAGTCGCGCGTCTTTCAGTATTAAGTGAGCTATGTTTA
CCGCTTGTAAAAGTTGGGGGAACATTCATTGCAATGAAAGGTGCCGCAGCAAAAGAAGAAATTGAAAATGGCAAGTATGC
TTTAGAGGTGCTAGGCGGGGATCTAAAAGAAATGTCTACGTTCCAATTACCGTTTGAAGAAAGTGAACGTAATATTTTAT
TAATCGAGAAAAAGCGCAAGACACCAAAGAAATATCCACGCAAACCGGGAACACCCAATAAATTACCTATTGAAAAATAA

Upstream 100 bases:

>100_bases
GGGCGTAAATCCAGCGGACATTTCCATTTTACTTGTGTATATTGAACAAGGAAAAATTGCGCGAGTATCGAACCAATAAA
TAGTAAGGAGATATTGTTAG

Downstream 100 bases:

>100_bases
ATCTTATTAGACGTAAGATTAAATTATATAAATGAAAACGTAAATGTTTCAAAGGAAACATTTTATGGAGAATAGTCAAT
AGGCCTAAAGGTGGTGGAAT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSKPFSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAAKEEIENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK

Sequences:

>Translated_239_residues
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSKPFSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAAKEEIENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK
>Mature_239_residues
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSKPFSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAAKEEIENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=217, Percent_Identity=30.4147465437788, Blast_Score=109, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27210; Mature: 27210

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSIT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
AAFYYDFSKPFSICDVGAGAGFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSD
HHHHHCCCCCCCCEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCLPLVKVGGTFIAMKGAAAKEE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHH
IENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK
HHCCCEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSIT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
AAFYYDFSKPFSICDVGAGAGFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSD
HHHHHCCCCCCCCEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCLPLVKVGGTFIAMKGAAAKEE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHH
IENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK
HHCCCEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA