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The map label for this gene is purA [H]

Identifier: 52145275

GI number: 52145275

Start: 5280807

End: 5282096

Strand: Reverse

Name: purA [H]

Synonym: BCZK5164

Alternate gene names: 52145275

Gene position: 5282096-5280807 (Counterclockwise)

Preceding gene: 52145276

Following gene: 52145274

Centisome position: 99.64

GC content: 39.3

Gene sequence:

>1290_bases
ATGTCTTCAGTAGTAGTTGTAGGAACACAATGGGGCGACGAAGGAAAAGGTAAAATCACTGATTTTCTTTCTGAGCATGC
GGAAGTAGTTGCAAGATATCAAGGTGGAAATAACGCAGGACATACAATTGTATTCGGCGGGGTTAAATATAAATTACACT
TAATTCCATCTGGTATTTTCTATAAAGAAAAAATTTGTGTAATCGGAAACGGCTTAGTAGTAGATCCGAAAGCGTTACTT
GAAGAGTTAAAATACTTACACGATCGTGGTGTAAGTACTGATAATTTACGCGTAAGTAACCGTGCGCACGTTATTTTACC
TTATCACTTAAAACAAGATGAGTTAGAAGAAGCAAGTAAAGGTGATGACAAAATCGGTACAACGAAAAAAGGTATCGGTC
CTGCATATATGGACAAAGCTGCTCGTATCGGTATCCGTATGGCTGATCTTCTAGACCGTGAAGCATTTAAAGAGAAGCTT
GAGCGCAATTTAGCACAAAAAAATCGTTTGTTTGAAAAAATGTACGATACAGAAGGTTTCAGTGTAGAAGAAATTTTCGA
AGAGTACTTCGAGTACGGACAACAAATCGCACAATATGTGTGTGATACGTCTGTTGTATTAAATGATGCATTAGATAACA
ATCACCGTGTATTATTTGAAGGTGCACAAGGTGTTATGCTTGATATCGACCATGGTACGTACCCATTCGTTACATCTTCT
AACCCGATTGCTGGTGGTGTAACAGTTGGAACTGGAGTTGGCCCTGCAAAAGTTACTCGCGTTGTAGGTGTATGTAAAGC
ATATACAAGCCGTGTTGGTGATGGTCCATTCCCTACTGAGCTTCATGATGAAATTGGTCATCAAATTCGTGAAGTTGGTC
GTGAGTATGGAACGACAACTGGTCGTCCACGCCGCGTAGGTTGGTTCGATAGCGTTGTTGTAAGACATGCACGTCGTGTT
AGTGGTTTAACAGATCTATCATTAAACTCTATCGACGTTCTAACTGGTATTCCAACACTTAAAATTTGTGTTGCTTACAA
ATGCGATGGAAAAGTTATCGATGAAGTTCCAGCAAACTTAAACATTTTAGCGAAATGTGAGCCTGTATACGAAGAGCTTC
CAGGTTGGACAGAAGATATTACTGGTGTAAGATCATTAGATGAGCTTCCTGAAAATGCACGAAAATACGTAGAGCGTGTT
TCTGAGTTAACAGGAATTCAATTATCTATGTTCTCAGTTGGACCAGATCGTAACCAAACAAATATCGTTCGTAACGTATA
CGAAGCTTAA

Upstream 100 bases:

>100_bases
TTATAAAAAAATGTTCGCTTTTTGGTTGACTTCTTTTTCCGTTTTGGTACAATTATATTGTTTGAATAGATCGTTTGAAA
ATTGCGGAGGTGCTTTAATA

Downstream 100 bases:

>100_bases
TTGATATTTTTGAGAAAAACTCATGTCCGCATGAGTTTTTTCTTATCTTTTATAAAAAAATAAAAAAAGGTATTGCAAAA
TATAAAGAAAACATGTTATG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALL
EELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDDKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKL
ERNLAQKNRLFEKMYDTEGFSVEEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERV
SELTGIQLSMFSVGPDRNQTNIVRNVYEA

Sequences:

>Translated_429_residues
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALL
EELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDDKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKL
ERNLAQKNRLFEKMYDTEGFSVEEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERV
SELTGIQLSMFSVGPDRNQTNIVRNVYEA
>Mature_428_residues
SSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALLE
ELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDDKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLE
RNLAQKNRLFEKMYDTEGFSVEEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSSN
PIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRVS
GLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERVS
ELTGIQLSMFSVGPDRNQTNIVRNVYEA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=43.3649289099526, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI22748717, Length=420, Percent_Identity=42.8571428571429, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI40316944, Length=391, Percent_Identity=41.6879795396419, Blast_Score=291, Evalue=6e-79,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=46.9767441860465, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=41.958041958042, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=41.958041958042, Blast_Score=328, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=41.6075650118203, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=43.1654676258993, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47467; Mature: 47336

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCE
YKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASK
EECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCHHHHHHHHC
GDDKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLERNLAQKNRLFEKMYDTEGF
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVEEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCCHHEEHHCCCCCCEEEEECCCEEEEEECCCCCCEEECC
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCHHCCCCCC
NILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERVSELTGIQLSMFSVGPDRNQT
EEEEECCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
NIVRNVYEA
HHHHHHHCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCE
YKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASK
EECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCHHHHHHHHC
GDDKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLERNLAQKNRLFEKMYDTEGF
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVEEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCCHHEEHHCCCCCCEEEEECCCEEEEEECCCCCCEEECC
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCHHCCCCCC
NILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERVSELTGIQLSMFSVGPDRNQT
EEEEECCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
NIVRNVYEA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA