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The map label for this gene is thiD [H]

Identifier: 52145222

GI number: 52145222

Start: 5218243

End: 5219067

Strand: Direct

Name: thiD [H]

Synonym: BCZK5110

Alternate gene names: 52145222

Gene position: 5218243-5219067 (Clockwise)

Preceding gene: 52145218

Following gene: 52145231

Centisome position: 98.44

GC content: 38.55

Gene sequence:

>825_bases
ATGACATTAAATAAAGCACTTACTATCGCTGGTTCTGACACAAGTGGCGGTGCTGGTATACAAGCAGATTTAAAAACATT
CCAAGAACTTGGTGTATACGGAATGACATCACTTACGACAATCGTAACGATGGATCCACATAACGGTTGGGCACATAACG
TATTCCCAATCCCAGCTTCTACATTAAAACCACAATTAGAAACGACAATTGAGGGCGTTGGCGTAGATGCTTTAAAAACA
GGTATGCTTGGATCGGTAGAAATTATTGAAATGGTTGCAGAAACAATCGAAAAACATAATTTCAAAAATGTAGTAGTTGA
CCCTGTTATGGTATGTAAAGGTGCTGATGAAGCATTACATCCTGAAACGAATGATTGCTTACGTGACGTTCTTGTTCCAA
AGGCATTAGTTGTAACACCAAACTTATTTGAAGCTTACCAATTAAGTGGCGTAAAGATTAATTCTCTTGAAGACATGAAA
GAAGCAGCAAAAAAGATCCACGCTTTAGGTGCGAAATACGTCTTAATTAAAGGCGGTAGCAAGCTTGGTACGGAAACTGC
AATCGACGTCCTATATGATGGAGAGACATTCGACCTTCTAGAATCAGAAAAAATTGATACGACAAATACACATGGCGCAG
GTTGCACATATTCTGCTGCAATTACAGCAGAACTTGCAAAAGGAAAATCTGTGAAAGAAGCAGTAAAAACTGCTAAAGAA
TTTATCACAGCTGCTATCCGTTATTCATTCAAGATCAATGAATACGTAGGCCCAACACATCACGGTGCATATCGTAAATT
CGTTGTATCAAAAGAACTTGCCTAA

Upstream 100 bases:

>100_bases
CTAACATAAATCATCTACTTTTCACTTTTTACTTATTCATAACATTGATATAATGGTGAAAACATAGTTTTCATTATATC
ACCTGAGGGGGCTTTTCATA

Downstream 100 bases:

>100_bases
CTAAACGAAAACACCCACGGGGTTCATATATTTTCTCCCTGTGGGTGTTTTTTATAAGCTCTTCAGGTCATCTTACCTTT
AAGAGGCAGAACTGACCTGA

Product: pyridoxal kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLNKALTIAGSDTSGGAGIQADLKTFQELGVYGMTSLTTIVTMDPHNGWAHNVFPIPASTLKPQLETTIEGVGVDALKT
GMLGSVEIIEMVAETIEKHNFKNVVVDPVMVCKGADEALHPETNDCLRDVLVPKALVVTPNLFEAYQLSGVKINSLEDMK
EAAKKIHALGAKYVLIKGGSKLGTETAIDVLYDGETFDLLESEKIDTTNTHGAGCTYSAAITAELAKGKSVKEAVKTAKE
FITAAIRYSFKINEYVGPTHHGAYRKFVVSKELA

Sequences:

>Translated_274_residues
MTLNKALTIAGSDTSGGAGIQADLKTFQELGVYGMTSLTTIVTMDPHNGWAHNVFPIPASTLKPQLETTIEGVGVDALKT
GMLGSVEIIEMVAETIEKHNFKNVVVDPVMVCKGADEALHPETNDCLRDVLVPKALVVTPNLFEAYQLSGVKINSLEDMK
EAAKKIHALGAKYVLIKGGSKLGTETAIDVLYDGETFDLLESEKIDTTNTHGAGCTYSAAITAELAKGKSVKEAVKTAKE
FITAAIRYSFKINEYVGPTHHGAYRKFVVSKELA
>Mature_273_residues
TLNKALTIAGSDTSGGAGIQADLKTFQELGVYGMTSLTTIVTMDPHNGWAHNVFPIPASTLKPQLETTIEGVGVDALKTG
MLGSVEIIEMVAETIEKHNFKNVVVDPVMVCKGADEALHPETNDCLRDVLVPKALVVTPNLFEAYQLSGVKINSLEDMKE
AAKKIHALGAKYVLIKGGSKLGTETAIDVLYDGETFDLLESEKIDTTNTHGAGCTYSAAITAELAKGKSVKEAVKTAKEF
ITAAIRYSFKINEYVGPTHHGAYRKFVVSKELA

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=37.3076923076923, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1788758, Length=259, Percent_Identity=25.0965250965251, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=33.5740072202166, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324517, Length=255, Percent_Identity=31.7647058823529, Blast_Score=92, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324997, Length=255, Percent_Identity=31.3725490196078, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 29374; Mature: 29243

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNKALTIAGSDTSGGAGIQADLKTFQELGVYGMTSLTTIVTMDPHNGWAHNVFPIPAS
CCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCHH
TLKPQLETTIEGVGVDALKTGMLGSVEIIEMVAETIEKHNFKNVVVDPVMVCKGADEALH
HHCCHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEECHHHEECCCCCCCC
PETNDCLRDVLVPKALVVTPNLFEAYQLSGVKINSLEDMKEAAKKIHALGAKYVLIKGGS
CCHHHHHHHHHCCCEEEECCCHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEEEECCC
KLGTETAIDVLYDGETFDLLESEKIDTTNTHGAGCTYSAAITAELAKGKSVKEAVKTAKE
CCCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHH
FITAAIRYSFKINEYVGPTHHGAYRKFVVSKELA
HHHHHHHHEEEECCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TLNKALTIAGSDTSGGAGIQADLKTFQELGVYGMTSLTTIVTMDPHNGWAHNVFPIPAS
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCHH
TLKPQLETTIEGVGVDALKTGMLGSVEIIEMVAETIEKHNFKNVVVDPVMVCKGADEALH
HHCCHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEECHHHEECCCCCCCC
PETNDCLRDVLVPKALVVTPNLFEAYQLSGVKINSLEDMKEAAKKIHALGAKYVLIKGGS
CCHHHHHHHHHCCCEEEECCCHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEEEECCC
KLGTETAIDVLYDGETFDLLESEKIDTTNTHGAGCTYSAAITAELAKGKSVKEAVKTAKE
CCCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHH
FITAAIRYSFKINEYVGPTHHGAYRKFVVSKELA
HHHHHHHHEEEECCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]