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The map label for this gene is acpD [H]

Identifier: 52145219

GI number: 52145219

Start: 5215050

End: 5215676

Strand: Reverse

Name: acpD [H]

Synonym: BCZK5107

Alternate gene names: 52145219

Gene position: 5215676-5215050 (Counterclockwise)

Preceding gene: 52145220

Following gene: 52145217

Centisome position: 98.39

GC content: 36.36

Gene sequence:

>627_bases
ATGACAAAAGTTTTATTCGTAAAAGCAAACAACCGTCCAGCGGAACAAGCAGTTAGTGTGAAATTATATGAAGCGTTTTT
AGCAAGTTACAAAGAAGCGCATCCAAATGATACAGTAGTAGAACTTGATTTATATAAAGAAGAATTACCATACGTAGGCG
TAGACATGATTAATGGTACATTCAAAGCAGGTAAAGGATTTGATTTAACAGAAGAAGAAGCAAAAGCAGTAGCAGTTGCT
GATAAGTATTTAAATCAATTTTTAGAAGCTGATAAAGTCGTTTTCGGTTTCCCATTATGGAACTTAACGATTCCAGCAGT
GTTACACACATATATTGATTATTTAAACCGTGCTGGTAAAACGTTTAAATATACGCCAGAAGGCCCAGTTGGTCTAATTG
GAGATAAGAAGATTGCATTATTAAACGCACGCGGTGGTGTATACTCTGAAGGTCCAGCAGCTGGAGCAGAAATGGCTGTT
AAGTATGTAGCAACTATGATGGGCTTCTTCGGCGCAACAAATATGGAAACAATAGTTATTGAAGGACATAACCAATTCCC
AGATAAAGCAGAAGAAATCATTACAGCTGGTCTTGAAGAAGCAGCTAAAGTAGCAAATAAATTTTAA

Upstream 100 bases:

>100_bases
AAGATAAAATATGTTTTGTAAGCAACAAACAACAATTACAAGGAATACCTTTTTTAAAAGCATATAACAACAGAAGAGCT
AGTAATAGGAGGAAAAGATA

Downstream 100 bases:

>100_bases
TTGAAATTATAATCGTCACTCAAAGAAACAGCATTAACGTTTATGTTAATGCTGTTTCTTTATATTTTTAGCTCATCAGC
AAGTTTTTCTAGTTGATTGC

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 3; FMN-dependent NADH-azo compound oxidoreductase 3 [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTKVLFVKANNRPAEQAVSVKLYEAFLASYKEAHPNDTVVELDLYKEELPYVGVDMINGTFKAGKGFDLTEEEAKAVAVA
DKYLNQFLEADKVVFGFPLWNLTIPAVLHTYIDYLNRAGKTFKYTPEGPVGLIGDKKIALLNARGGVYSEGPAAGAEMAV
KYVATMMGFFGATNMETIVIEGHNQFPDKAEEIITAGLEEAAKVANKF

Sequences:

>Translated_208_residues
MTKVLFVKANNRPAEQAVSVKLYEAFLASYKEAHPNDTVVELDLYKEELPYVGVDMINGTFKAGKGFDLTEEEAKAVAVA
DKYLNQFLEADKVVFGFPLWNLTIPAVLHTYIDYLNRAGKTFKYTPEGPVGLIGDKKIALLNARGGVYSEGPAAGAEMAV
KYVATMMGFFGATNMETIVIEGHNQFPDKAEEIITAGLEEAAKVANKF
>Mature_207_residues
TKVLFVKANNRPAEQAVSVKLYEAFLASYKEAHPNDTVVELDLYKEELPYVGVDMINGTFKAGKGFDLTEEEAKAVAVAD
KYLNQFLEADKVVFGFPLWNLTIPAVLHTYIDYLNRAGKTFKYTPEGPVGLIGDKKIALLNARGGVYSEGPAAGAEMAVK
YVATMMGFFGATNMETIVIEGHNQFPDKAEEIITAGLEEAAKVANKF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=190, Percent_Identity=30, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 22768; Mature: 22637

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLFVKANNRPAEQAVSVKLYEAFLASYKEAHPNDTVVELDLYKEELPYVGVDMINGT
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCEEECCCE
FKAGKGFDLTEEEAKAVAVADKYLNQFLEADKVVFGFPLWNLTIPAVLHTYIDYLNRAGK
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCC
TFKYTPEGPVGLIGDKKIALLNARGGVYSEGPAAGAEMAVKYVATMMGFFGATNMETIVI
EEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
EGHNQFPDKAEEIITAGLEEAAKVANKF
ECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKVLFVKANNRPAEQAVSVKLYEAFLASYKEAHPNDTVVELDLYKEELPYVGVDMINGT
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCEEECCCE
FKAGKGFDLTEEEAKAVAVADKYLNQFLEADKVVFGFPLWNLTIPAVLHTYIDYLNRAGK
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCC
TFKYTPEGPVGLIGDKKIALLNARGGVYSEGPAAGAEMAVKYVATMMGFFGATNMETIVI
EEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
EGHNQFPDKAEEIITAGLEEAAKVANKF
ECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]