| Definition | Bacillus cereus E33L, complete genome. |
|---|---|
| Accession | NC_006274 |
| Length | 5,300,915 |
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The map label for this gene is pbp2 [H]
Identifier: 52142993
GI number: 52142993
Start: 2374226
End: 2376352
Strand: Direct
Name: pbp2 [H]
Synonym: BCZK2246
Alternate gene names: 52142993
Gene position: 2374226-2376352 (Clockwise)
Preceding gene: 52142995
Following gene: 52142992
Centisome position: 44.79
GC content: 34.74
Gene sequence:
>2127_bases ATGAAAGAACAAAAAGGAAAGAAGCAGAAGACATATATATCTATTCGATTAAATATAATGTTCCTTTGCATATTTTTACT ATTCTCAGCTATTATTATGCAGCTAGGAAAAGTACAAATCGTTGAGGGAGAGGCTTATAAGAATCAAGTGGAAAACAGTC AAAATGCAACAACTAGCATTCCAGTTCCGCGTGGACAGATTTTAGATCGAGAAGGGAAAACGGTCGTTAATAATAAGTCT CTTCGTACAATTACGTATACAAGGGTGAAGGGGATTACGAGTGACGATATTTTAAAAACAGCAAAAGACTTGGCGAAAGT ACTTGAAATGTCTGAACAAGATATTAATAAATTAACTGATATCGATAAAAAAGATTTTTGGATGCAGTTGAATACGAAAC GTGCGGATACAAAGATTGCTAAAAAAGATATAGAAAAGTTTAAAGAAGAGGGAATAGAGGGAAAAGAGTTAGATAAAAAA ATAGAAGATTTAAGACGAAGTCGCGTTACAGAAGTAGAATTGGGTGAGTTAACAGAACAAGATTTAAAGGTGTTAGCTAT TAAAAGTAAAATGAGTTCAGGTTATCAACTAACACCACAAATTATTAAAAAAGATGTAACAGATGAAGAGTATGCGCGTA TAAGTGAAAACCTTGCGATTTTTCCAGGAGTAGATGCAACTGTTGATTGGGAACGTAATTATGTAAATGGTAATTTATTT CGTTCTGTATTAGGAAATATTACGAGTAATGAAGAAGGATTACCGAAAGAAAATTTAGATTCTTATTTAGTACGTGGATA TAACCGGAATGATCGTGTCGGAAAAAGTTATATTGAACAACGATATGAAGATGTATTACACGGTACAAAAGAAGAAGTGA AAAACATAACTGATAAATCAGGAAACATTATAAATACAGAAGTAATCGCTAAAGGAAAAAGTGGAAAAAGTTTAACATTA ACGATTGATATGGAATTACAAAAGAAAGTGGAAGAAAGTATAGAGAAAAACTTGAGAGCTTTTAAGAGTTCTGAGCCGCT ACTCGATCGAGCTCTTGTCGTCATGACGAATCCAAATAACGGACAGATTTTATCTATGGCAGGCAAAAAGATTGTAGAAA GAGAAGGGAAAACAGAGATTGAGGATTTGGCATTAGGCAATATGACAACTTCGTATGAGCTAGGTTCTGCTGTAAAAGGT GCTACTTTATTAACTGGATATGAGACAGGAGCAATACATCCAGGGGATCAATTTTATGATGCACCGATGAAATTTAAAGG TACACAAGCTAAGAAATCGTGGAATGTATCTGGATTTGGTAATATTAATGATTTACGAGCTTTACAAGTATCTTCGAATG TATACATGTTCCAGACAGCTTTAAAAATTGCAGGAGTTAATTACGTACCAAATGGCTCATTGGATATTAAACAAGGAGCA TTTGATACGATGCGCTATTATTTCAAGCAATTTGGTTTAGGCGTCCCAACAGGTATCGATTTACCGAATGAAATAATTGG ACAAACTAGAAAAGTAGATAGTCAACCTGGGTTTTTACTAGACTTTTCCATCGGTCAGTATGATACGTATACGCCGTTGC AATTGGCACAATATATTTCAACGATTGCTAATGGTGGATATAGAATGCAACCTCAAATTGTACAAGAAATACGAGAACAA TCAATAAAAGACGAGGTTGGTAAAGTTATACGCTCTATAGAACCTGTCGTATTAAATCGAGTTGATATGAAGAAAGAACA TATTGACCGGATAAAAGAAGGGTTTAGATGGGTATTCCAAGAAGGGGATGGAACTGGCGTAAAGTATTTCAAAAATGCGC CATATAAACCCGCTGGAAAGACAGGGACAGCCCAAACTGTATATGGCGGAGATGATCCAATTGGTAGAAATGCAAAAGGA GAACGTATGGAATGTTATAACTTAACGTTAGTCGGATATGCTCCATATGATAATCCAGAAGTAGCGTTTTCTGTAGTAGT CCCATGGCTTCATAATGACAAAAGTGGAATTAATTCTATAATAGGAAAGGAAGTTTTAGATGTATACTTTGATTTGAAAA AGCAACGTATGCATGATGAAGCTACTAGTACAGATAGTTCCAAGTAA
Upstream 100 bases:
>100_bases AAAAAGATAAGACATTATTTATAACCGAATAATGGTTTGTAATATATAGGGAACGTTTAAGTATTAGTGAGAAGTTTCAG AAGAATTATATGGAGGATTT
Downstream 100 bases:
>100_bases AATTAGTAATATTCGAGTGCTTTTCTCAGGGGAGAGACCCTGTTTATATAAAGGCATTTATAAGTAAAAAGATGAAATGT GAAATAAGAGATAGCAGCGG
Product: penicillin-binding protein 2
Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate
Alternate protein names: NA
Number of amino acids: Translated: 708; Mature: 708
Protein sequence:
>708_residues MKEQKGKKQKTYISIRLNIMFLCIFLLFSAIIMQLGKVQIVEGEAYKNQVENSQNATTSIPVPRGQILDREGKTVVNNKS LRTITYTRVKGITSDDILKTAKDLAKVLEMSEQDINKLTDIDKKDFWMQLNTKRADTKIAKKDIEKFKEEGIEGKELDKK IEDLRRSRVTEVELGELTEQDLKVLAIKSKMSSGYQLTPQIIKKDVTDEEYARISENLAIFPGVDATVDWERNYVNGNLF RSVLGNITSNEEGLPKENLDSYLVRGYNRNDRVGKSYIEQRYEDVLHGTKEEVKNITDKSGNIINTEVIAKGKSGKSLTL TIDMELQKKVEESIEKNLRAFKSSEPLLDRALVVMTNPNNGQILSMAGKKIVEREGKTEIEDLALGNMTTSYELGSAVKG ATLLTGYETGAIHPGDQFYDAPMKFKGTQAKKSWNVSGFGNINDLRALQVSSNVYMFQTALKIAGVNYVPNGSLDIKQGA FDTMRYYFKQFGLGVPTGIDLPNEIIGQTRKVDSQPGFLLDFSIGQYDTYTPLQLAQYISTIANGGYRMQPQIVQEIREQ SIKDEVGKVIRSIEPVVLNRVDMKKEHIDRIKEGFRWVFQEGDGTGVKYFKNAPYKPAGKTGTAQTVYGGDDPIGRNAKG ERMECYNLTLVGYAPYDNPEVAFSVVVPWLHNDKSGINSIIGKEVLDVYFDLKKQRMHDEATSTDSSK
Sequences:
>Translated_708_residues MKEQKGKKQKTYISIRLNIMFLCIFLLFSAIIMQLGKVQIVEGEAYKNQVENSQNATTSIPVPRGQILDREGKTVVNNKS LRTITYTRVKGITSDDILKTAKDLAKVLEMSEQDINKLTDIDKKDFWMQLNTKRADTKIAKKDIEKFKEEGIEGKELDKK IEDLRRSRVTEVELGELTEQDLKVLAIKSKMSSGYQLTPQIIKKDVTDEEYARISENLAIFPGVDATVDWERNYVNGNLF RSVLGNITSNEEGLPKENLDSYLVRGYNRNDRVGKSYIEQRYEDVLHGTKEEVKNITDKSGNIINTEVIAKGKSGKSLTL TIDMELQKKVEESIEKNLRAFKSSEPLLDRALVVMTNPNNGQILSMAGKKIVEREGKTEIEDLALGNMTTSYELGSAVKG ATLLTGYETGAIHPGDQFYDAPMKFKGTQAKKSWNVSGFGNINDLRALQVSSNVYMFQTALKIAGVNYVPNGSLDIKQGA FDTMRYYFKQFGLGVPTGIDLPNEIIGQTRKVDSQPGFLLDFSIGQYDTYTPLQLAQYISTIANGGYRMQPQIVQEIREQ SIKDEVGKVIRSIEPVVLNRVDMKKEHIDRIKEGFRWVFQEGDGTGVKYFKNAPYKPAGKTGTAQTVYGGDDPIGRNAKG ERMECYNLTLVGYAPYDNPEVAFSVVVPWLHNDKSGINSIIGKEVLDVYFDLKKQRMHDEATSTDSSK >Mature_708_residues MKEQKGKKQKTYISIRLNIMFLCIFLLFSAIIMQLGKVQIVEGEAYKNQVENSQNATTSIPVPRGQILDREGKTVVNNKS LRTITYTRVKGITSDDILKTAKDLAKVLEMSEQDINKLTDIDKKDFWMQLNTKRADTKIAKKDIEKFKEEGIEGKELDKK IEDLRRSRVTEVELGELTEQDLKVLAIKSKMSSGYQLTPQIIKKDVTDEEYARISENLAIFPGVDATVDWERNYVNGNLF RSVLGNITSNEEGLPKENLDSYLVRGYNRNDRVGKSYIEQRYEDVLHGTKEEVKNITDKSGNIINTEVIAKGKSGKSLTL TIDMELQKKVEESIEKNLRAFKSSEPLLDRALVVMTNPNNGQILSMAGKKIVEREGKTEIEDLALGNMTTSYELGSAVKG ATLLTGYETGAIHPGDQFYDAPMKFKGTQAKKSWNVSGFGNINDLRALQVSSNVYMFQTALKIAGVNYVPNGSLDIKQGA FDTMRYYFKQFGLGVPTGIDLPNEIIGQTRKVDSQPGFLLDFSIGQYDTYTPLQLAQYISTIANGGYRMQPQIVQEIREQ SIKDEVGKVIRSIEPVVLNRVDMKKEHIDRIKEGFRWVFQEGDGTGVKYFKNAPYKPAGKTGTAQTVYGGDDPIGRNAKG ERMECYNLTLVGYAPYDNPEVAFSVVVPWLHNDKSGINSIIGKEVLDVYFDLKKQRMHDEATSTDSSK
Specific function: Cell Wall Formation; Pbp-2 Is Responsible For The Determination Of The Rod Shape Of The Cell. Its Synthesize Cross- Linked Peptidoglycan From The Lipid Intermediates. [C]
COG id: COG0768
COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2
Gene ontology:
Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786854, Length=530, Percent_Identity=25.0943396226415, Blast_Score=141, Evalue=1e-34, Organism=Escherichia coli, GI1786272, Length=427, Percent_Identity=25.9953161592506, Blast_Score=66, Evalue=6e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR005311 - InterPro: IPR001460 [H]
Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]
EC number: 2.4.1.129
Molecular weight: Translated: 79610; Mature: 79610
Theoretical pI: Translated: 8.39; Mature: 8.39
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKEQKGKKQKTYISIRLNIMFLCIFLLFSAIIMQLGKVQIVEGEAYKNQVENSQNATTSI CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCEEC PVPRGQILDREGKTVVNNKSLRTITYTRVKGITSDDILKTAKDLAKVLEMSEQDINKLTD CCCCCCEECCCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC IDKKDFWMQLNTKRADTKIAKKDIEKFKEEGIEGKELDKKIEDLRRSRVTEVELGELTEQ CCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHH DLKVLAIKSKMSSGYQLTPQIIKKDVTDEEYARISENLAIFPGVDATVDWERNYVNGNLF HHHEEEEHHHHCCCCEECHHHHHHCCCHHHHHHHHCCEEEECCCCCEECCCCCCCCHHHH RSVLGNITSNEEGLPKENLDSYLVRGYNRNDRVGKSYIEQRYEDVLHGTKEEVKNITDKS HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC GNIINTEVIAKGKSGKSLTLTIDMELQKKVEESIEKNLRAFKSSEPLLDRALVVMTNPNN CCEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC GQILSMAGKKIVEREGKTEIEDLALGNMTTSYELGSAVKGATLLTGYETGAIHPGDQFYD CEEEEHHHHHHHHHCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCHHCC APMKFKGTQAKKSWNVSGFGNINDLRALQVSSNVYMFQTALKIAGVNYVPNGSLDIKQGA CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCCCCCCCCH FDTMRYYFKQFGLGVPTGIDLPNEIIGQTRKVDSQPGFLLDFSIGQYDTYTPLQLAQYIS HHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHH TIANGGYRMQPQIVQEIREQSIKDEVGKVIRSIEPVVLNRVDMKKEHIDRIKEGFRWVFQ HHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEE EGDGTGVKYFKNAPYKPAGKTGTAQTVYGGDDPIGRNAKGERMECYNLTLVGYAPYDNPE CCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCC VAFSVVVPWLHNDKSGINSIIGKEVLDVYFDLKKQRMHDEATSTDSSK EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC >Mature Secondary Structure MKEQKGKKQKTYISIRLNIMFLCIFLLFSAIIMQLGKVQIVEGEAYKNQVENSQNATTSI CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCEEC PVPRGQILDREGKTVVNNKSLRTITYTRVKGITSDDILKTAKDLAKVLEMSEQDINKLTD CCCCCCEECCCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC IDKKDFWMQLNTKRADTKIAKKDIEKFKEEGIEGKELDKKIEDLRRSRVTEVELGELTEQ CCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHH DLKVLAIKSKMSSGYQLTPQIIKKDVTDEEYARISENLAIFPGVDATVDWERNYVNGNLF HHHEEEEHHHHCCCCEECHHHHHHCCCHHHHHHHHCCEEEECCCCCEECCCCCCCCHHHH RSVLGNITSNEEGLPKENLDSYLVRGYNRNDRVGKSYIEQRYEDVLHGTKEEVKNITDKS HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC GNIINTEVIAKGKSGKSLTLTIDMELQKKVEESIEKNLRAFKSSEPLLDRALVVMTNPNN CCEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC GQILSMAGKKIVEREGKTEIEDLALGNMTTSYELGSAVKGATLLTGYETGAIHPGDQFYD CEEEEHHHHHHHHHCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCHHCC APMKFKGTQAKKSWNVSGFGNINDLRALQVSSNVYMFQTALKIAGVNYVPNGSLDIKQGA CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCCCCCCCCH FDTMRYYFKQFGLGVPTGIDLPNEIIGQTRKVDSQPGFLLDFSIGQYDTYTPLQLAQYIS HHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHH TIANGGYRMQPQIVQEIREQSIKDEVGKVIRSIEPVVLNRVDMKKEHIDRIKEGFRWVFQ HHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEE EGDGTGVKYFKNAPYKPAGKTGTAQTVYGGDDPIGRNAKGERMECYNLTLVGYAPYDNPE CCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCC VAFSVVVPWLHNDKSGINSIIGKEVLDVYFDLKKQRMHDEATSTDSSK EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol
Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 8969508; 9384377 [H]