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The map label for this gene is pfs [H]

Identifier: 52142935

GI number: 52142935

Start: 2439660

End: 2440370

Strand: Direct

Name: pfs [H]

Synonym: BCZK2305

Alternate gene names: 52142935

Gene position: 2439660-2440370 (Clockwise)

Preceding gene: 52142937

Following gene: 52142934

Centisome position: 46.02

GC content: 34.74

Gene sequence:

>711_bases
ATGAAACGTATTGCAATCGTAGCCGCTTGGGAACCTGAACTTACATATTTACATCAATATTATCCAAGTGAACGCATTGA
AAAAAGAGCCGCTTGGGAATTTCATTTTCATACTATAAATGATCTGGAAATTATTTCAGTTATAACTGGTGTTGGGAAGG
TAAGTTGTGCTAGTTGTGCACAATTATTAATTAGTGAGTTTCAGCCAGATGAGTTGTTTATGACAGGGATATGTGGGAGT
TTATCAAATAAGGTGAAAAATGGTCATATTGTAGTGGCACTAAAAGCCATACAACACGATGTCACTGCTGCTGGTTCGGG
CGAAGATGTTTTTAACTTATATAATGGTAGAACAGCACCTATTGAAACAACAAAATCACTTGTAAGAAGAATAAAAAAAA
TACGATCGTATGATCCGATTCATTTCGGGACATTTTTATCTGGAGATCAACGTATCCGTAGTTCGGAAATGAGATATTTA
CTCCATACTGTATATGGGGCATTGGCAGTTGATCAAGAAGTGGCAGCTTTTGCTTATGTATGTCAAATCAATAAAAAACC
TTTTTTATGTTTAAAAGCTGCTTCAGATCAAGCAAATGATAAAACGAAAGAGGAACAAAAAATCTTTAAAATGTTAGCAT
GTGAACGAGCTTGTGAGCATTTAATTGCTTTTTTACGTGTGTATGAGATCAATGTAGTAAATCATAGATAG

Upstream 100 bases:

>100_bases
TACGAAATTTATATGAGAAATATAAAGAGTGGATTCCAGATTATAAAAGAGAAAGCATAGATAAATTATTTAAATGACAG
TGAGGGAAGTGAAGTTTGTA

Downstream 100 bases:

>100_bases
TAAGAGGGGCTTAATATTGGAAAGTACAAATAAAATAGCTATTTTGGGCGCGAACGGAAAAGCCGGAAAAATTCTTGTAA
ATGAAGCGTTAGAAAAGGGA

Product: S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKRIAIVAAWEPELTYLHQYYPSERIEKRAAWEFHFHTINDLEIISVITGVGKVSCASCAQLLISEFQPDELFMTGICGS
LSNKVKNGHIVVALKAIQHDVTAAGSGEDVFNLYNGRTAPIETTKSLVRRIKKIRSYDPIHFGTFLSGDQRIRSSEMRYL
LHTVYGALAVDQEVAAFAYVCQINKKPFLCLKAASDQANDKTKEEQKIFKMLACERACEHLIAFLRVYEINVVNHR

Sequences:

>Translated_236_residues
MKRIAIVAAWEPELTYLHQYYPSERIEKRAAWEFHFHTINDLEIISVITGVGKVSCASCAQLLISEFQPDELFMTGICGS
LSNKVKNGHIVVALKAIQHDVTAAGSGEDVFNLYNGRTAPIETTKSLVRRIKKIRSYDPIHFGTFLSGDQRIRSSEMRYL
LHTVYGALAVDQEVAAFAYVCQINKKPFLCLKAASDQANDKTKEEQKIFKMLACERACEHLIAFLRVYEINVVNHR
>Mature_236_residues
MKRIAIVAAWEPELTYLHQYYPSERIEKRAAWEFHFHTINDLEIISVITGVGKVSCASCAQLLISEFQPDELFMTGICGS
LSNKVKNGHIVVALKAIQHDVTAAGSGEDVFNLYNGRTAPIETTKSLVRRIKKIRSYDPIHFGTFLSGDQRIRSSEMRYL
LHTVYGALAVDQEVAAFAYVCQINKKPFLCLKAASDQANDKTKEEQKIFKMLACERACEHLIAFLRVYEINVVNHR

Specific function: Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) [H]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786354, Length=209, Percent_Identity=26.7942583732057, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =3.2.2.9 [H]

Molecular weight: Translated: 26726; Mature: 26726

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAIVAAWEPELTYLHQYYPSERIEKRAAWEFHFHTINDLEIISVITGVGKVSCASCA
CCEEEEEEECCCCHHHHHHHCCHHHHHHHHCEEEEEEECCCHHHHHHHHCCCCCCHHHHH
QLLISEFQPDELFMTGICGSLSNKVKNGHIVVALKAIQHDVTAAGSGEDVFNLYNGRTAP
HHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCCHHHHHCCCCCCC
IETTKSLVRRIKKIRSYDPIHFGTFLSGDQRIRSSEMRYLLHTVYGALAVDQEVAAFAYV
HHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQINKKPFLCLKAASDQANDKTKEEQKIFKMLACERACEHLIAFLRVYEINVVNHR
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
>Mature Secondary Structure
MKRIAIVAAWEPELTYLHQYYPSERIEKRAAWEFHFHTINDLEIISVITGVGKVSCASCA
CCEEEEEEECCCCHHHHHHHCCHHHHHHHHCEEEEEEECCCHHHHHHHHCCCCCCHHHHH
QLLISEFQPDELFMTGICGSLSNKVKNGHIVVALKAIQHDVTAAGSGEDVFNLYNGRTAP
HHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCCHHHHHCCCCCCC
IETTKSLVRRIKKIRSYDPIHFGTFLSGDQRIRSSEMRYLLHTVYGALAVDQEVAAFAYV
HHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQINKKPFLCLKAASDQANDKTKEEQKIFKMLACERACEHLIAFLRVYEINVVNHR
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9332349; 9665876 [H]