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The map label for this gene is ddl [H]

Identifier: 52142884

GI number: 52142884

Start: 2489765

End: 2490679

Strand: Direct

Name: ddl [H]

Synonym: BCZK2356

Alternate gene names: 52142884

Gene position: 2489765-2490679 (Clockwise)

Preceding gene: 52142885

Following gene: 52142882

Centisome position: 46.97

GC content: 33.88

Gene sequence:

>915_bases
ATGAGAATTGGCGTTATTATGGGAGGAGTATCCTCTGAAAAACAAGTATCAATTATGACTGGAAATGAAATGATTGCACA
TTTAGATAAAAATAAATATGAAATAGTACCAATTACGTTAAATGAAAAAATGGATTTAATCGAAAAAGCAAAAGACATTG
ATTTTGCACTGCTCGCATTACATGGAAAATACGGAGAAGATGGGACAGTTCAAGGAACGCTTGAAAGTTTAGGTATTCCT
TATAGCGGGAGCAATATGTTATCTAGCGGTATATGTATGGACAAAAACATGTCAAAAAAGATTTTACGTTATGAAGGAAT
AGAAACACCAGATTGGATTGAACTTACAAAAATGGAGGATTTGAATTTTGATGAGTTAGATAAGTTAGGATTTCCATTAG
TGGTAAAACCAAACTCTGGTGGTTCAAGTGTTGGGGTAAAGATTGTCTATGATAAAGACGAATTAATTTCAATGCTGGAA
ACTGTGTTCGAATGGGATTCTGAAGTAGTAATAGAAAAGTATATTAAAGGTGATGAGATTACATGTTCCATTTTTGATGG
AAAACAGTTACCTATCATTTCAATTCGACATGCGGCTGAGTTCTTTGATTACAATGCGAAATATGATGATGCAGGTACTA
TTGAAGAAGTTATCGAACTTCCAGCTGAACTTAAGGAACGTGTAAACAAAGCGTCACTGGCTTGCTATAAAGCATTAAAA
TGTAGTGTTTATGCAAGGGTTGATATGATGGTGAAGGACGGTATTCCATATGTAATGGAGGTTAATACATTACCAGGGAT
GACACAAGCAAGTTTACTACCAAAAAGTGCAGATGCAGCGGGAATTCATTATAGTAAACTATTAGATATGATTATTGAAA
CTTCATTAAGAGTAAGAAAAGAAGAAGGGTTTTAA

Upstream 100 bases:

>100_bases
ACATTTCGATTAGGATTTTCACGTCTGAAAGAAGAAGAAATAGTTCGGGGAATCAAAATAATCGGTGATACAATAAAAAA
TGAAATTTGGAGTTGAGGAA

Downstream 100 bases:

>100_bases
GTAACGGTATTATGAATACTTTTGACAGTAACAAGAAGTATGTTATGATGAATACGATACGAAAATAAAATATTTATCCA
CTAGGGGGGCCTATTATAGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLE
TVFEWDSEVVIEKYIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF

Sequences:

>Translated_304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLE
TVFEWDSEVVIEKYIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF
>Mature_304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLE
TVFEWDSEVVIEKYIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=301, Percent_Identity=36.5448504983389, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=29.512893982808, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33850; Mature: 33850

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLAL
CEEEEEECCCCCCCEEEEEECCCEEEEECCCCEEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMED
ECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECC
LNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLETVFEWDSEVVIEKYIKGDEI
CCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCCE
TCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASLACYKALK
EEEEECCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRK
HEEEEEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGF
CCCC
>Mature Secondary Structure
MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLAL
CEEEEEECCCCCCCEEEEEECCCEEEEECCCCEEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMED
ECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECC
LNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLETVFEWDSEVVIEKYIKGDEI
CCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCCE
TCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASLACYKALK
EEEEECCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRK
HEEEEEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA