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The map label for this gene is tenA [H]

Identifier: 52142882

GI number: 52142882

Start: 2490981

End: 2491670

Strand: Direct

Name: tenA [H]

Synonym: BCZK2357

Alternate gene names: 52142882

Gene position: 2490981-2491670 (Clockwise)

Preceding gene: 52142884

Following gene: 52142883

Centisome position: 46.99

GC content: 36.09

Gene sequence:

>690_bases
ATGACTTTTTCACAATCATTACGTAAAGAAGTAGATTCAATTTGGGAAGCTAGTTTTCATCATCCTTTTGTAAAAAAACT
CGGTGAAGGAACGTTAGATTTAGCTAGTTTTCGCTATTACGTGCTTCAAGATTCCTATTATTTGAGTCACTTCGCTAGAG
TACAAACATTAGGAGCTGCAAAAGCGCTTGAATTAGAAACGACTGCTCGCATGGCACATCATGCTCAAAATACATATGAG
GCGGAATTATCTTTACATGAGAACTTTGCGAAGAAACTAGGGATTACACAAGAAGAAAAAGATAATTTTATCCCTGCTCC
AACTGCATATGCATATACTTCACATATGTACCGTGCTGCGTATGAAGGACATTTAGGCGATATTATTGCAGCAATTTTAC
CTTGCTATTGGCTGTATTATGAAATTGGTGAGCGTTTAAAAGAGTGTCAGCCAGAAGAGCCGATTTATAACGAATGGATT
TCTGCTTATGGATCTGATTGGTTCCGAACATTAGTTGAAGAGCAAATTACACGATTAGATGCAATAGCTGAAAAGGTGAC
AGAGTCTGATCGTAAGCGTATGAAACAGCATTTTATTATAAGTAGTCAATATGAATATTCATTTTGGGAAATGGCTTATA
CTTTAGAAAAGTGGCCGGTAGATACAGAAGTAAAAGATGTAATAAGATAA

Upstream 100 bases:

>100_bases
ATTTCACTTTCTTTACGCCTGTAAAGAAAGTTTTTTTATTATAAAGCGTTAATTACTGTGGATAAATACTTATATGAAAT
GGATTATTGGAGGGAATAAA

Downstream 100 bases:

>100_bases
AGAAAAGAGGTATTGCTATACGTATGTATGGACAATACCTCTTTTTTAGGTTTTTGTAAAATTATTGTAAAAATCATAAA
TAAAACACTCCTTTATATTG

Product: transcriptional activator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDSYYLSHFARVQTLGAAKALELETTARMAHHAQNTYE
AELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWI
SAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEVKDVIR

Sequences:

>Translated_229_residues
MTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDSYYLSHFARVQTLGAAKALELETTARMAHHAQNTYE
AELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWI
SAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
>Mature_228_residues
TFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDSYYLSHFARVQTLGAAKALELETTARMAHHAQNTYEA
ELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWIS
AYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEVKDVIR

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=202, Percent_Identity=26.7326732673267, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325378, Length=205, Percent_Identity=25.8536585365854, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324997, Length=201, Percent_Identity=21.8905472636816, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26898; Mature: 26767

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDSYYLSHFARVQTLGAA
CCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
KALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHH
YEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYGSDWFRTLVEEQITRLD
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
AIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDSYYLSHFARVQTLGAA
CHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
KALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHH
YEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYGSDWFRTLVEEQITRLD
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
AIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]