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The map label for this gene is cwlJ [H]

Identifier: 52141731

GI number: 52141731

Start: 3640289

End: 3641086

Strand: Direct

Name: cwlJ [H]

Synonym: BCZK3515

Alternate gene names: 52141731

Gene position: 3640289-3641086 (Clockwise)

Preceding gene: 52141738

Following gene: 52141732

Centisome position: 68.67

GC content: 35.96

Gene sequence:

>798_bases
ATGAAATTATTAAAAATAAAACATATAATCCCTTTATCTGCGGCTGCAATTACATTCGTATGTAGTCAAAGTACAGCTGA
GGCTTCTACCATTCATACAGTAAAAAAGAATGATACACTTTGGGGCATTAGTAAACAATACGGCGTTTCAATACAATCCA
TTAAACAGGAAAATCATAAAGGAAACGATCAAACTTTCATTGGCGAACAGTTACATATTCCAGGTGCCACGAACTCAAAT
AAAATTACCGTTCGTCAAAACGTCAAACCTGCAAACATTAGTGACCAAATTATATATCAAGTTCAATCGGGAGACTCATT
AGAAACGATAGCAAAGCGTTACAATGTTACCGTTCAATCTATAAAGCAAATTAACAATACAGTCGGAAACAAGCTTTATA
CAGGACAACATTTGAAAATCAACTCAAGCATTTCAGAAAAAGAAAAAGACTTAATGGCACGCTTAGTTACTGCTGAAGCA
GGTGGCGAATCGTATAAAGGAAAAGTGGCTGTAGCAAAAGTTATCCTAAACCGTGTAAATGCAAAAGGGTTTCCAAATAC
AATAACAGGCGTTATTTATGAACCTATTACATACGGCTATGCATTTACTCCTGTTACAGATGGAAGAATTAATCATCCTG
CAAGCCCAGAAGCAAAAATGGCAGTAGAAGAGGCTATCTCCACAAATGGAATACATTCCGATTGGCTTTATTTTTACAAT
CCAAAAACATCAACAGACAAATGGATTACGACACGTCAAACAGTGGCAGTAATTGGTAACCACGTCTTCGCTAAATAA

Upstream 100 bases:

>100_bases
ATATGTGCTAATATACTTACATTAATATATTAAGTATATAAATATCCAAAACTACATTACTGAACAGCTTCACCTTAATT
TTTTCTTAGGAGGATTTACA

Downstream 100 bases:

>100_bases
CATATAAAAAAACAGACCTCTTCACTTAAAAGGTCTGTTTTTTTATATGTAAGATTTTATTAATCATATGACATAGTGAT
CTTTTAAAACTATCTCTTCT

Product: cell wall hydrolase; spore-cortex lytic enzyme

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKLLKIKHIIPLSAAAITFVCSQSTAEASTIHTVKKNDTLWGISKQYGVSIQSIKQENHKGNDQTFIGEQLHIPGATNSN
KITVRQNVKPANISDQIIYQVQSGDSLETIAKRYNVTVQSIKQINNTVGNKLYTGQHLKINSSISEKEKDLMARLVTAEA
GGESYKGKVAVAKVILNRVNAKGFPNTITGVIYEPITYGYAFTPVTDGRINHPASPEAKMAVEEAISTNGIHSDWLYFYN
PKTSTDKWITTRQTVAVIGNHVFAK

Sequences:

>Translated_265_residues
MKLLKIKHIIPLSAAAITFVCSQSTAEASTIHTVKKNDTLWGISKQYGVSIQSIKQENHKGNDQTFIGEQLHIPGATNSN
KITVRQNVKPANISDQIIYQVQSGDSLETIAKRYNVTVQSIKQINNTVGNKLYTGQHLKINSSISEKEKDLMARLVTAEA
GGESYKGKVAVAKVILNRVNAKGFPNTITGVIYEPITYGYAFTPVTDGRINHPASPEAKMAVEEAISTNGIHSDWLYFYN
PKTSTDKWITTRQTVAVIGNHVFAK
>Mature_265_residues
MKLLKIKHIIPLSAAAITFVCSQSTAEASTIHTVKKNDTLWGISKQYGVSIQSIKQENHKGNDQTFIGEQLHIPGATNSN
KITVRQNVKPANISDQIIYQVQSGDSLETIAKRYNVTVQSIKQINNTVGNKLYTGQHLKINSSISEKEKDLMARLVTAEA
GGESYKGKVAVAKVILNRVNAKGFPNTITGVIYEPITYGYAFTPVTDGRINHPASPEAKMAVEEAISTNGIHSDWLYFYN
PKTSTDKWITTRQTVAVIGNHVFAK

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786405, Length=127, Percent_Identity=30.7086614173228, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105 [H]

Pfam domain/function: PF07486 Hydrolase_2 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 29145; Mature: 29145

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKIKHIIPLSAAAITFVCSQSTAEASTIHTVKKNDTLWGISKQYGVSIQSIKQENHK
CCEEEEEEECCCHHHHEEEEECCCCCCHHHEEEEECCCEEEECCHHHCCCHHHHHHHCCC
GNDQTFIGEQLHIPGATNSNKITVRQNVKPANISDQIIYQVQSGDSLETIAKRYNVTVQS
CCCCEEECCEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHH
IKQINNTVGNKLYTGQHLKINSSISEKEKDLMARLVTAEAGGESYKGKVAVAKVILNRVN
HHHHHHHHCCCEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
AKGFPNTITGVIYEPITYGYAFTPVTDGRINHPASPEAKMAVEEAISTNGIHSDWLYFYN
CCCCCCCHHHEEECCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEC
PKTSTDKWITTRQTVAVIGNHVFAK
CCCCCCCEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLLKIKHIIPLSAAAITFVCSQSTAEASTIHTVKKNDTLWGISKQYGVSIQSIKQENHK
CCEEEEEEECCCHHHHEEEEECCCCCCHHHEEEEECCCEEEECCHHHCCCHHHHHHHCCC
GNDQTFIGEQLHIPGATNSNKITVRQNVKPANISDQIIYQVQSGDSLETIAKRYNVTVQS
CCCCEEECCEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHH
IKQINNTVGNKLYTGQHLKINSSISEKEKDLMARLVTAEAGGESYKGKVAVAKVILNRVN
HHHHHHHHCCCEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
AKGFPNTITGVIYEPITYGYAFTPVTDGRINHPASPEAKMAVEEAISTNGIHSDWLYFYN
CCCCCCCHHHEEECCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEC
PKTSTDKWITTRQTVAVIGNHVFAK
CCCCCCCEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]