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The map label for this gene is ymfH [H]

Identifier: 52141701

GI number: 52141701

Start: 3677223

End: 3678509

Strand: Reverse

Name: ymfH [H]

Synonym: BCZK3546

Alternate gene names: 52141701

Gene position: 3678509-3677223 (Counterclockwise)

Preceding gene: 52141700

Following gene: 52141703

Centisome position: 69.39

GC content: 33.02

Gene sequence:

>1287_bases
ATGGAGAAAATTGTTTATGAGCAATTAAAAGAGACACTCTATTATGAAAAACTTCCTAATGGATTAGATGTATATATTTT
ACCGAAGCAAGGGTTTAATAAAACATTTGCAACGTTTACGACAAAATATGGTTCTGTGGATAATACATTCGTACCACTAG
GTAAAGAAGAAATGATTCGTGTACCTGATGGGATTGCTCATTTTCTTGAGCATAAATTATTTGAAAAAGAAGATCATGAT
GCTTTCCAATTGTTTAGTAAACAAGGGGCATCGGCAAATGCTTTCACATCTTTCACAAGAACAGCTTACCTTTTTTCATG
TACATCAAACGTAGAACAAAATTTAAATACATTGTTAAACTTCGTACAAGAGCCTTACTTCTCTGAAAAAACCGTTGAAA
AAGAGAAGGGTATTATTGGGCAAGAAATTCAAATGTATCAAGATAATCCAGATTGGCGCTTATATTTTGGATTAATTGAT
AGCTTGTTTGTAAAGCACCCGATTAAAATTGATATTGCAGGGACGATCGAGTCTATTAGTAAAATTACGAAAGACCTACT
ATATGAATGTTATGAAACGTTTTATCATCCAAGCAATATGTTAATGTTTGTTGTAGGTGCAATTGATCCAGAGAAAACGA
TGGATTTGATACGTGAAAATCAAGCGAAAAAAGATTATAAAAACCAACCAGAAATAGTACGTTCATTCGAAGAAGAACCA
GATGAGGTAAATGAAAAGAAGAAAATTATTTCAATGCCTGTACAAACTCCGAAATGTTTAGTTGGTATTAAAGCGACAAA
CTTAAAAGAAAAAGGGGAAGCCCTTTTAAAACAAGAAATTGCGCTTACGTTACTTTTAGATTATTTATTTGGGAAAAGCT
CCGTTCATTATGAATCTTTATATAATGAAGGGCTCATTGATGATTCGTTCTCGTATGATTATACAGAAGAGAATAACTTT
GGTTTTGCAATGGTTGGCGGCGATACGAAGCAACCTGATGAGCTGGAAGAGCGTTTGAAAGGTATTTTATTAAATACAAA
TTATGATCAATTAGATGAGGCGGCATTAGAACGAGTAAAGAAAAAGAAAATAGGTGGCTTTTTACGTTCCTTGAATTCAC
CCGAATATATTGCAAATCAATTTACCCGATATGCGTTTAATGAATCGAGTCTGTTTGATGCATTGACTGTATTAGAAGGT
CTAACGGTTCAAGATTTACAAGAAGTAGCTCAATTACTATTATCAGAAGAGAAAATGAGTGTTTGCCAAGTTTTACCGAA
AAAATAA

Upstream 100 bases:

>100_bases
TACAGGCATTGAAAGTGTGACGAAAGAAGAGATTGTGAAGGTTGCTAAAAATATTGAACTAGATACAATTTACTTTTTAC
ACGGAACGGAGGGAGAATAA

Downstream 100 bases:

>100_bases
AAGGTTTTATAATAATACCCTCATCTTATCATTGATAAATTGTAAGATGAGGGTATTATTTATTTGTAGGGAAACGATTT
TTTTGAGATAAGAGAGGAAT

Product: insulinase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEKIVYEQLKETLYYEKLPNGLDVYILPKQGFNKTFATFTTKYGSVDNTFVPLGKEEMIRVPDGIAHFLEHKLFEKEDHD
AFQLFSKQGASANAFTSFTRTAYLFSCTSNVEQNLNTLLNFVQEPYFSEKTVEKEKGIIGQEIQMYQDNPDWRLYFGLID
SLFVKHPIKIDIAGTIESISKITKDLLYECYETFYHPSNMLMFVVGAIDPEKTMDLIRENQAKKDYKNQPEIVRSFEEEP
DEVNEKKKIISMPVQTPKCLVGIKATNLKEKGEALLKQEIALTLLLDYLFGKSSVHYESLYNEGLIDDSFSYDYTEENNF
GFAMVGGDTKQPDELEERLKGILLNTNYDQLDEAALERVKKKKIGGFLRSLNSPEYIANQFTRYAFNESSLFDALTVLEG
LTVQDLQEVAQLLLSEEKMSVCQVLPKK

Sequences:

>Translated_428_residues
MEKIVYEQLKETLYYEKLPNGLDVYILPKQGFNKTFATFTTKYGSVDNTFVPLGKEEMIRVPDGIAHFLEHKLFEKEDHD
AFQLFSKQGASANAFTSFTRTAYLFSCTSNVEQNLNTLLNFVQEPYFSEKTVEKEKGIIGQEIQMYQDNPDWRLYFGLID
SLFVKHPIKIDIAGTIESISKITKDLLYECYETFYHPSNMLMFVVGAIDPEKTMDLIRENQAKKDYKNQPEIVRSFEEEP
DEVNEKKKIISMPVQTPKCLVGIKATNLKEKGEALLKQEIALTLLLDYLFGKSSVHYESLYNEGLIDDSFSYDYTEENNF
GFAMVGGDTKQPDELEERLKGILLNTNYDQLDEAALERVKKKKIGGFLRSLNSPEYIANQFTRYAFNESSLFDALTVLEG
LTVQDLQEVAQLLLSEEKMSVCQVLPKK
>Mature_428_residues
MEKIVYEQLKETLYYEKLPNGLDVYILPKQGFNKTFATFTTKYGSVDNTFVPLGKEEMIRVPDGIAHFLEHKLFEKEDHD
AFQLFSKQGASANAFTSFTRTAYLFSCTSNVEQNLNTLLNFVQEPYFSEKTVEKEKGIIGQEIQMYQDNPDWRLYFGLID
SLFVKHPIKIDIAGTIESISKITKDLLYECYETFYHPSNMLMFVVGAIDPEKTMDLIRENQAKKDYKNQPEIVRSFEEEP
DEVNEKKKIISMPVQTPKCLVGIKATNLKEKGEALLKQEIALTLLLDYLFGKSSVHYESLYNEGLIDDSFSYDYTEENNF
GFAMVGGDTKQPDELEERLKGILLNTNYDQLDEAALERVKKKKIGGFLRSLNSPEYIANQFTRYAFNESSLFDALTVLEG
LTVQDLQEVAQLLLSEEKMSVCQVLPKK

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Escherichia coli, GI1787770, Length=240, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71999683, Length=427, Percent_Identity=23.4192037470726, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 49184; Mature: 49184

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIVYEQLKETLYYEKLPNGLDVYILPKQGFNKTFATFTTKYGSVDNTFVPLGKEEMIR
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHEE
VPDGIAHFLEHKLFEKEDHDAFQLFSKQGASANAFTSFTRTAYLFSCTSNVEQNLNTLLN
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHH
FVQEPYFSEKTVEKEKGIIGQEIQMYQDNPDWRLYFGLIDSLFVKHPIKIDIAGTIESIS
HHHCCCCCHHHHHHHCCCCCCHHHEECCCCCCEEHHHHHHHHHHCCCEEEEECHHHHHHH
KITKDLLYECYETFYHPSNMLMFVVGAIDPEKTMDLIRENQAKKDYKNQPEIVRSFEEEP
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
DEVNEKKKIISMPVQTPKCLVGIKATNLKEKGEALLKQEIALTLLLDYLFGKSSVHYESL
HHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YNEGLIDDSFSYDYTEENNFGFAMVGGDTKQPDELEERLKGILLNTNYDQLDEAALERVK
HCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCEEECCCHHHHHHHHHHHHH
KKKIGGFLRSLNSPEYIANQFTRYAFNESSLFDALTVLEGLTVQDLQEVAQLLLSEEKMS
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VCQVLPKK
HHHHCCCC
>Mature Secondary Structure
MEKIVYEQLKETLYYEKLPNGLDVYILPKQGFNKTFATFTTKYGSVDNTFVPLGKEEMIR
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHEE
VPDGIAHFLEHKLFEKEDHDAFQLFSKQGASANAFTSFTRTAYLFSCTSNVEQNLNTLLN
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHH
FVQEPYFSEKTVEKEKGIIGQEIQMYQDNPDWRLYFGLIDSLFVKHPIKIDIAGTIESIS
HHHCCCCCHHHHHHHCCCCCCHHHEECCCCCCEEHHHHHHHHHHCCCEEEEECHHHHHHH
KITKDLLYECYETFYHPSNMLMFVVGAIDPEKTMDLIRENQAKKDYKNQPEIVRSFEEEP
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
DEVNEKKKIISMPVQTPKCLVGIKATNLKEKGEALLKQEIALTLLLDYLFGKSSVHYESL
HHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YNEGLIDDSFSYDYTEENNFGFAMVGGDTKQPDELEERLKGILLNTNYDQLDEAALERVK
HCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCEEECCCHHHHHHHHHHHHH
KKKIGGFLRSLNSPEYIANQFTRYAFNESSLFDALTVLEGLTVQDLQEVAQLLLSEEKMS
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VCQVLPKK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]