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The map label for this gene is ymfF [H]

Identifier: 52141700

GI number: 52141700

Start: 3678510

End: 3679784

Strand: Reverse

Name: ymfF [H]

Synonym: BCZK3547

Alternate gene names: 52141700

Gene position: 3679784-3678510 (Counterclockwise)

Preceding gene: 52141698

Following gene: 52141701

Centisome position: 69.42

GC content: 34.2

Gene sequence:

>1275_bases
ATGAAACTGATGGAACAACAGTTACATGAGTTAGGTGGTTTGCGAGTACATATTATTCCAACTAATAAATATAAAACAAA
TACATTTGTATTTCGTTTGAAAGCACCTTTAAATGAGGAGACGGTGACAGAGCGTGCCTTATTGCCATACGTATTGCAAA
GTGCGACAGAAAAGCTTCCTTCTGTAATTCGTCTTCGTCAATATTTAGAAGAATTATATGGATCTTCTTTGGCAGTAGAT
GTAAGTAAAAAAGGAGAAGATCATATCATCTCTATTTATGTAGACATTGCAAATGAAGTATATTTACATGAAGCACCACC
GTTATTTGAAAAAGCGCTTTCTATGTTGTCAGATATCGTTTTACATCCAGCAACAGAGGGGAATGGTTTTCTGCCTTCTA
TTGTAGAAAGTGAAAAGAGAGCGCTCTTGCAACGAATTGAAGCGACTTATGATGATAAAATGCGTTATGCAAACGAGCGT
TTAATTGAAGAAATGTGCAAAGTAGAACCGTATCGTTTAAGCGCAAATGGGAAAAAAGAGAGTGTTTCTTCTATTACAAA
TGAAAGTTTGTATCAATATTATCAAAAAGTGTTAGCTGAAGATGAAATGGATCTATACATTATTGGTGATATTTCAGAAA
ATGCAGTTGATCTTGTAAGTAAGTATTTTTCTATTTCAGCGCGTCCTGTGAGGGAAAGAAATGTACTTCTTCATAAGAGA
AATAATGAAGAAAAAGAAGTTGTGGAAAAACAAGAATTAAAACAAAGTAAATTACACATCGGTTATCGTACGTTTATTAC
GTATAAAGATGAAGATTATTTTGCACTGCAATTGTTTAATGGATTGTTTGGAGGATTTTCTCATTCGAAGTTATTCGTTA
ATGTTCGTGAAAAAAATAGTTTAGCGTACTATGCAGCATCCCGCTTTGAAAGTCATAAAGGCTTATTGTTTGTTATGTCA
GGAATTGAAGCGAAAAATTATGAAAAAGCTGTTGAGATTATTAAAGAGCAAATGCTTGCAATGCAAAATGGTGATTTTTC
TGAAGAAGAGATGCATCAAACGAAAAGTGTCATTCAAAATCAAATATTAGAAGCAATTGATACACCGCGCGGATTTGTGG
AAATGCTGTATCATGGTATTATTTCAGACCGTACGCGTCCGGTTGAAGAATGGCTTACAGGCATTGAAAGTGTGACGAAA
GAAGAGATTGTGAAGGTTGCTAAAAATATTGAACTAGATACAATTTACTTTTTACACGGAACGGAGGGAGAATAA

Upstream 100 bases:

>100_bases
GGCCTAATGTAAGTATAGTGAGGAAGAAAAGTTGATTTTTCATTTCTAAAAACTGTATATTGAAGAGGAATATTCCTATA
TACGTAAAGGAGGGCTATAA

Downstream 100 bases:

>100_bases
ATGGAGAAAATTGTTTATGAGCAATTAAAAGAGACACTCTATTATGAAAAACTTCCTAATGGATTAGATGTATATATTTT
ACCGAAGCAAGGGTTTAATA

Product: insulinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKLMEQQLHELGGLRVHIIPTNKYKTNTFVFRLKAPLNEETVTERALLPYVLQSATEKLPSVIRLRQYLEELYGSSLAVD
VSKKGEDHIISIYVDIANEVYLHEAPPLFEKALSMLSDIVLHPATEGNGFLPSIVESEKRALLQRIEATYDDKMRYANER
LIEEMCKVEPYRLSANGKKESVSSITNESLYQYYQKVLAEDEMDLYIIGDISENAVDLVSKYFSISARPVRERNVLLHKR
NNEEKEVVEKQELKQSKLHIGYRTFITYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNSLAYYAASRFESHKGLLFVMS
GIEAKNYEKAVEIIKEQMLAMQNGDFSEEEMHQTKSVIQNQILEAIDTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTK
EEIVKVAKNIELDTIYFLHGTEGE

Sequences:

>Translated_424_residues
MKLMEQQLHELGGLRVHIIPTNKYKTNTFVFRLKAPLNEETVTERALLPYVLQSATEKLPSVIRLRQYLEELYGSSLAVD
VSKKGEDHIISIYVDIANEVYLHEAPPLFEKALSMLSDIVLHPATEGNGFLPSIVESEKRALLQRIEATYDDKMRYANER
LIEEMCKVEPYRLSANGKKESVSSITNESLYQYYQKVLAEDEMDLYIIGDISENAVDLVSKYFSISARPVRERNVLLHKR
NNEEKEVVEKQELKQSKLHIGYRTFITYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNSLAYYAASRFESHKGLLFVMS
GIEAKNYEKAVEIIKEQMLAMQNGDFSEEEMHQTKSVIQNQILEAIDTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTK
EEIVKVAKNIELDTIYFLHGTEGE
>Mature_424_residues
MKLMEQQLHELGGLRVHIIPTNKYKTNTFVFRLKAPLNEETVTERALLPYVLQSATEKLPSVIRLRQYLEELYGSSLAVD
VSKKGEDHIISIYVDIANEVYLHEAPPLFEKALSMLSDIVLHPATEGNGFLPSIVESEKRALLQRIEATYDDKMRYANER
LIEEMCKVEPYRLSANGKKESVSSITNESLYQYYQKVLAEDEMDLYIIGDISENAVDLVSKYFSISARPVRERNVLLHKR
NNEEKEVVEKQELKQSKLHIGYRTFITYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNSLAYYAASRFESHKGLLFVMS
GIEAKNYEKAVEIIKEQMLAMQNGDFSEEEMHQTKSVIQNQILEAIDTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTK
EEIVKVAKNIELDTIYFLHGTEGE

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR007863 [H]

Pfam domain/function: PF05193 Peptidase_M16_C [H]

EC number: NA

Molecular weight: Translated: 48860; Mature: 48860

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMEQQLHELGGLRVHIIPTNKYKTNTFVFRLKAPLNEETVTERALLPYVLQSATEKLP
CCHHHHHHHHHCCCEEEEEECCCEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
SVIRLRQYLEELYGSSLAVDVSKKGEDHIISIYVDIANEVYLHEAPPLFEKALSMLSDIV
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHHHHH
LHPATEGNGFLPSIVESEKRALLQRIEATYDDKMRYANERLIEEMCKVEPYRLSANGKKE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
SVSSITNESLYQYYQKVLAEDEMDLYIIGDISENAVDLVSKYFSISARPVRERNVLLHKR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHCCEEEECC
NNEEKEVVEKQELKQSKLHIGYRTFITYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNS
CCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCC
LAYYAASRFESHKGLLFVMSGIEAKNYEKAVEIIKEQMLAMQNGDFSEEEMHQTKSVIQN
EEEHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QILEAIDTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTKEEIVKVAKNIELDTIYFLHG
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
TEGE
CCCC
>Mature Secondary Structure
MKLMEQQLHELGGLRVHIIPTNKYKTNTFVFRLKAPLNEETVTERALLPYVLQSATEKLP
CCHHHHHHHHHCCCEEEEEECCCEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
SVIRLRQYLEELYGSSLAVDVSKKGEDHIISIYVDIANEVYLHEAPPLFEKALSMLSDIV
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHHHHH
LHPATEGNGFLPSIVESEKRALLQRIEATYDDKMRYANERLIEEMCKVEPYRLSANGKKE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
SVSSITNESLYQYYQKVLAEDEMDLYIIGDISENAVDLVSKYFSISARPVRERNVLLHKR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHCCEEEECC
NNEEKEVVEKQELKQSKLHIGYRTFITYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNS
CCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCC
LAYYAASRFESHKGLLFVMSGIEAKNYEKAVEIIKEQMLAMQNGDFSEEEMHQTKSVIQN
EEEHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QILEAIDTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTKEEIVKVAKNIELDTIYFLHG
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
TEGE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]