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The map label for this gene is pnpA [H]

Identifier: 52141682

GI number: 52141682

Start: 3700012

End: 3702150

Strand: Reverse

Name: pnpA [H]

Synonym: BCZK3566

Alternate gene names: 52141682

Gene position: 3702150-3700012 (Counterclockwise)

Preceding gene: 52141679

Following gene: 52141681

Centisome position: 69.84

GC content: 39.22

Gene sequence:

>2139_bases
ATGAGTCAAGAAAAGCAAGTCTTCTCGATAGATTTAGCTGGTCGCCAGCTAACAGTTGAAACAGGTCAGCTTGCAAAGCA
AGCGAACGGAGCAGTATTAGTAAGATATGGCGATACAGCGGTTCTATCTACAGCAACTGCATCAAAAGAAGCAAAAAATG
TAGATTTCTTCCCACTTACAGTAAACTATGAAGAGCGTTTATATGCAGTCGGAAAAATTCCTGGCGGTTTCATTAAACGT
GAAGGTCGTCCAAGTGAAAAAGCAATTTTGGCAAGTCGTTTAATCGACCGTCCAATTCGTCCGCTTTTCGCTGATGGTTT
CCGTAACGAAGTACAAGTTGTCAGCATCGTAATGAGTGTTGATCAAGATTGTTCTTCTGAAATGGCAGCTATGCTTGGTT
CTTCATTAGCGTTATCGATTTCAGATATTCCATTTGAAGGTCCAATTGCGGGTGCAACAGTTGGTCGTATTAACGGCGAA
TTCGTTATCAACCCAACAGTAGAACAGCAAGAACAAAGTGACATTCACCTTGTTGTAGCTGGTACGAAAGATGCAATTAA
CATGGTTGAAGCAGGAGCAGATCAAGTGCCTGAGGAAACAATGTTAGAAGCAATTATGTTTGGTCATGACGAAATTAAAC
GTCTAATTGCATTCCAAGAAGAGATTGTACAAGCTGTAGGTAAAGAGAAATCAGAAGTGAAACTTTATGAAGTTGACGCT
GATCTTAACCAAGCTGTACGTGAAATGGCTGAGAAGGATATGCATTCTGCAATTCAAGTACATGAGAAACATGCACGTGA
AGATGCAATTAACGAAGTGAAAAAGCGTGTAATTGAGCATTACGAAGCGCAGGAAGCTGACGCTGATACTTTAGGACAAG
TAAATGAGATTTTATATAAAATTGTAAAAGAAGAAGTACGTCGTCTTATTACAGTTGAAAAAATCCGCCCAGATGGCCGT
AAAGGTGACGAAATCCGTCCATTAGCATCAGAGGTTGGCATTTTATCTCGTACACACGGTTCTGGTTTGTTCACTCGTGG
ACAAACACAAGCATTAAGTATTTGTACATTAGGTGCATTAGGCGATGTGCAAATTTTAGACGGTCTTGGTGTAGAAGAAT
CAAAACGCTTTATGCACCATTACAATTTCCCATCATTTAGTGTTGGTGAAACAAGACCGATGCGTGGACCAGGTCGTCGT
GAAATCGGTCACGGTGCACTAGGAGAACGTGCTCTTGAACCTGTAATTCCATCTGAAAAAGACTTCCCATACACAGTACG
TCTTGTGTCTGAAGTATTAGAATCAAATGGTTCTACTTCACAAGCAAGTATTTGTGGTAGTACTTTAGCAATGATGGATG
CTGGTGTTCCACTTAAAGCTCCAGTTGCAGGTATTGCAATGGGGCTAGTTAAAACTGGTGAGCATTACACAATTTTATCT
GATATTCAAGGTATGGAAGATCATTTAGGTGATATGGACTTTAAAGTAGCAGGTACAGCACATGGTGTAACTGCATTACA
AATGGACATTAAAATCGATGGTCTATCTCGTGAAATTTTAGAAGAGGCATTACAACAAGCGAAAGTTGGTCGTGTGCACA
TTCTAAATCATATGTTATCTGTTATTGCAGAGCCACGCACTGAATTATCAGCGTACGCTCCAAAGATTATTACAATGACA
ATTAACCCAGATAAAATCCGTGACGTTATCGGACCAAGCGGTAAACAAATCAATAAAATTATTGAAGAAACTGGCGTTAA
AATTGACATCGAGCAAGATGGTACAGTATTCATTTCTTCAATAAATCAAGAAATGAACGACAAAGCTAAGAAAATTATCG
AAGATATCGTTCGCGAAGTACAAGTAGGTGAAATCTACGAAGGAAAAGTGAAACGTGTTGAGAAATTCGGTGCTTTCGTT
GAATTATTCAGCGGTAAAGATGGATTAGTTCACATTTCTGAACTTGCACTTGAGCGTGTAGGTAAAGTAGAAGACGTTGT
GAAAATCGGTGATGTAATTACAGTTAAAGTTATCGAGATTGACAAGCAAGGTCGCGTGAATCTATCTAGAAAAGTATTGC
TAAAAGAAGAGCAAGAAAAAGAAGCTGCTAAAGAAGAAAATAAACAAGAGCAGCAATAA

Upstream 100 bases:

>100_bases
TAGCTTACTTCATTTTCGGTAATACTAGAGGTAGAATATTGTGATATACTATCCATTTACATAGAAGAATTGAATACTTT
AAATTGAGAGGGGTACTTAA

Downstream 100 bases:

>100_bases
GCAAAAGCCAGTTTAACTGGCTTTTTTTGTTATGTAAAAATATGATTTACATATATTTTTATGTCAGTATAGGTATAGTT
TTCATTATAACGCTCAAATT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 712; Mature: 711

Protein sequence:

>712_residues
MSQEKQVFSIDLAGRQLTVETGQLAKQANGAVLVRYGDTAVLSTATASKEAKNVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRINGE
FVINPTVEQQEQSDIHLVVAGTKDAINMVEAGADQVPEETMLEAIMFGHDEIKRLIAFQEEIVQAVGKEKSEVKLYEVDA
DLNQAVREMAEKDMHSAIQVHEKHAREDAINEVKKRVIEHYEAQEADADTLGQVNEILYKIVKEEVRRLITVEKIRPDGR
KGDEIRPLASEVGILSRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRR
EIGHGALGERALEPVIPSEKDFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKTGEHYTILS
DIQGMEDHLGDMDFKVAGTAHGVTALQMDIKIDGLSREILEEALQQAKVGRVHILNHMLSVIAEPRTELSAYAPKIITMT
INPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISSINQEMNDKAKKIIEDIVREVQVGEIYEGKVKRVEKFGAFV
ELFSGKDGLVHISELALERVGKVEDVVKIGDVITVKVIEIDKQGRVNLSRKVLLKEEQEKEAAKEENKQEQQ

Sequences:

>Translated_712_residues
MSQEKQVFSIDLAGRQLTVETGQLAKQANGAVLVRYGDTAVLSTATASKEAKNVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRINGE
FVINPTVEQQEQSDIHLVVAGTKDAINMVEAGADQVPEETMLEAIMFGHDEIKRLIAFQEEIVQAVGKEKSEVKLYEVDA
DLNQAVREMAEKDMHSAIQVHEKHAREDAINEVKKRVIEHYEAQEADADTLGQVNEILYKIVKEEVRRLITVEKIRPDGR
KGDEIRPLASEVGILSRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRR
EIGHGALGERALEPVIPSEKDFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKTGEHYTILS
DIQGMEDHLGDMDFKVAGTAHGVTALQMDIKIDGLSREILEEALQQAKVGRVHILNHMLSVIAEPRTELSAYAPKIITMT
INPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISSINQEMNDKAKKIIEDIVREVQVGEIYEGKVKRVEKFGAFV
ELFSGKDGLVHISELALERVGKVEDVVKIGDVITVKVIEIDKQGRVNLSRKVLLKEEQEKEAAKEENKQEQQ
>Mature_711_residues
SQEKQVFSIDLAGRQLTVETGQLAKQANGAVLVRYGDTAVLSTATASKEAKNVDFFPLTVNYEERLYAVGKIPGGFIKRE
GRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRINGEF
VINPTVEQQEQSDIHLVVAGTKDAINMVEAGADQVPEETMLEAIMFGHDEIKRLIAFQEEIVQAVGKEKSEVKLYEVDAD
LNQAVREMAEKDMHSAIQVHEKHAREDAINEVKKRVIEHYEAQEADADTLGQVNEILYKIVKEEVRRLITVEKIRPDGRK
GDEIRPLASEVGILSRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRRE
IGHGALGERALEPVIPSEKDFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKTGEHYTILSD
IQGMEDHLGDMDFKVAGTAHGVTALQMDIKIDGLSREILEEALQQAKVGRVHILNHMLSVIAEPRTELSAYAPKIITMTI
NPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISSINQEMNDKAKKIIEDIVREVQVGEIYEGKVKRVEKFGAFVE
LFSGKDGLVHISELALERVGKVEDVVKIGDVITVKVIEIDKQGRVNLSRKVLLKEEQEKEAAKEENKQEQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=36.2994350282486, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI9506689, Length=233, Percent_Identity=26.1802575107296, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=50.6588579795022, Blast_Score=665, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=716, Percent_Identity=33.5195530726257, Blast_Score=355, Evalue=6e-98,
Organism=Saccharomyces cerevisiae, GI6320850, Length=72, Percent_Identity=44.4444444444444, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=39.3767705382436, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=39.3767705382436, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=39.3767705382436, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI161079377, Length=682, Percent_Identity=37.9765395894428, Blast_Score=424, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78182; Mature: 78051

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKQVFSIDLAGRQLTVETGQLAKQANGAVLVRYGDTAVLSTATASKEAKNVDFFPLT
CCCCCEEEEEEECCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
DQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRINGEFVINPTVEQQEQSDIHLVVA
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCEEEEEE
GTKDAINMVEAGADQVPEETMLEAIMFGHDEIKRLIAFQEEIVQAVGKEKSEVKLYEVDA
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
DLNQAVREMAEKDMHSAIQVHEKHAREDAINEVKKRVIEHYEAQEADADTLGQVNEILYK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IVKEEVRRLITVEKIRPDGRKGDEIRPLASEVGILSRTHGSGLFTRGQTQALSICTLGAL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHEECCCCCCEECCCCCEEEEEEECCC
GDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRREIGHGALGERALEPVIPSEK
CCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
DFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKTGEHYTILS
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEHH
DIQGMEDHLGDMDFKVAGTAHGVTALQMDIKIDGLSREILEEALQQAKVGRVHILNHMLS
HHCCHHHHCCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHH
VIAEPRTELSAYAPKIITMTINPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISS
HHHCCHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEH
INQEMNDKAKKIIEDIVREVQVGEIYEGKVKRVEKFGAFVELFSGKDGLVHISELALERV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
GKVEDVVKIGDVITVKVIEIDKQGRVNLSRKVLLKEEQEKEAAKEENKQEQQ
CCHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQEKQVFSIDLAGRQLTVETGQLAKQANGAVLVRYGDTAVLSTATASKEAKNVDFFPLT
CCCCEEEEEEECCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
DQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRINGEFVINPTVEQQEQSDIHLVVA
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCEEEEEE
GTKDAINMVEAGADQVPEETMLEAIMFGHDEIKRLIAFQEEIVQAVGKEKSEVKLYEVDA
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
DLNQAVREMAEKDMHSAIQVHEKHAREDAINEVKKRVIEHYEAQEADADTLGQVNEILYK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IVKEEVRRLITVEKIRPDGRKGDEIRPLASEVGILSRTHGSGLFTRGQTQALSICTLGAL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHEECCCCCCEECCCCCEEEEEEECCC
GDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRREIGHGALGERALEPVIPSEK
CCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
DFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKTGEHYTILS
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEHH
DIQGMEDHLGDMDFKVAGTAHGVTALQMDIKIDGLSREILEEALQQAKVGRVHILNHMLS
HHCCHHHHCCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHH
VIAEPRTELSAYAPKIITMTINPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISS
HHHCCHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEH
INQEMNDKAKKIIEDIVREVQVGEIYEGKVKRVEKFGAFVELFSGKDGLVHISELALERV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
GKVEDVVKIGDVITVKVIEIDKQGRVNLSRKVLLKEEQEKEAAKEENKQEQQ
CCHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA