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The map label for this gene is proS [H]

Identifier: 52141669

GI number: 52141669

Start: 3714369

End: 3716069

Strand: Reverse

Name: proS [H]

Synonym: BCZK3578

Alternate gene names: 52141669

Gene position: 3716069-3714369 (Counterclockwise)

Preceding gene: 52141668

Following gene: 52141670

Centisome position: 70.1

GC content: 38.51

Gene sequence:

>1701_bases
ATGAAACAAAGTATGGTATTCAGTCCTACATTACGTGAAGTTCCAGCTGATGCGGAGATTAAGAGTCATCAGTTATTACT
TCGTGCAGGTTTTATGCGTCAAAATGCTTCTGGTATTTATAGTTTTCTACCATTTGGATTAAAAGTACTACACAAAGTAG
AACGTATCGTTCGAGAAGAGATGGAGCGCGCAGGTGCCGTAGAATTATTAATGCCAGCGATGCAAGCTGCAGAATTATGG
CAAGAGTCAGGTCGTTGGTATTCTTACGGATCTGAATTAATGCGTATGAAAGATCGTAACGCTCGTGAATTTGCATTAGG
AGCGACACATGAAGAAGTAATTACAGATCTTGTACGTGATGAAGTGAAATCGTATAAAAAATTACCGTTAACATTATATC
AAATTCAAACAAAATTCCGTGATGAACAAAGACCTCGTTTCGGTTTATTACGTGGAAGAGAGTTTCTAATGAAAGATGCA
TACTCTTTCCATGCTACGCAAGAGAGCTTAGATGAAGTGTACGATCGCTTATACAAAGCATACTCTAACATCTTTGCTCG
TTGTGGCTTGAATTTCCGTGCGGTTATTGCTGATTCTGGAGCAATGGGTGGAAAAGATACACATGAATTTATGGTATTAT
CTGATGTTGGTGAAGATACAATTGCATACTCTGATACATCTGATTATGCAGCGAACATCGAAATGGCTCCTGTTGTAGCT
ACGTATACGAAGAGTGACGAAGCAGAAAAAGAGCTTGAAAAAGTAGCAACACCAGACCAAAAAGCAATTGAAGAAGTATC
TGCATTCTTAAACATCGAAGCTGACAAGTGCATTAAGTCTATGGTATTTAAAGTAGATGAGAAATTAGTAGTGGTACTTG
TTCGTGGTGATCATGAAGTAAACGATGTAAAAGTGAAAAATGTATACGGTGCTTCAGTTGTTGAGCTTGCCTCTCATGAA
GAAGTAAAAGAATTATTAAATTGTGAAGTTGGTTCATTAGGACCGATTGGTGTAAATGGTGATATCGAAATTATCGCTGA
TCACGCTGTAGCATCAATTGTCAACGGATGTTCAGGAGCGAACGAAGAAGGATTCCATTATGTAAATGTAAATCCAGAAC
GTGACTTTAAAGTAAGTCAATATACGGATTTACGCTTCATTCAAGAAGGAGACCAATCTCCAGACGGAAACGGGACAATT
CTTTTCGCACGCGGAATTGAAGTTGGTCATGTATTCAAATTAGGAACTCGTTATAGTGAAGCAATGAACGCAACATTCCT
AGATGAAAACGGAAAAACACAACCACTTATTATGGGTTGTTACGGCATTGGTGTGTCTCGTACAGTGGCAGCAATTGCAG
AGCAGTTTAATGATGAGAACGGTTTAGTTTGGCCAAAAGCTGTAGCACCGTTCCATGTGCATGTAATTCCAGTGAATATG
AAATCTGATGCACAACGTGAAATGGGTGAAAACATCTACAACTCATTACAAGAGCAAGGATATGAAGTATTACTAGATGA
TCGTGCAGAACGTGCAGGTGTTAAATTTGCTGATGCTGATTTATTCGGCCTTCCAGTTCGCGTGACAGTTGGTAAAAAAG
CAGACGAAGGTATTGTAGAAGTGAAAGTACGTGCTACAGGTGAGTCTGAAGAAATAAAAGTAGAAGAACTTCAAACATAT
ATTGCTAATATTTTAAAATAG

Upstream 100 bases:

>100_bases
AAACTTATAATGAAATAGAGCTCGTAGAATAAAATCCCTCACCTAACTCGAGGTGAGGGGTTTTCCTGCGTGTAAGAGTA
AAATGAAGAGGTGCGAATAA

Downstream 100 bases:

>100_bases
GAAGAGGTACCTCGTAATGAGGTACCTTCTCTTTCTTTGTAAACGTATTTTGTAAAAGAAGAAGGGAGAAATCTTTCTTT
GTAGTACGTTTCAATTTATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 1; ProRS 1 [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREEMERAGAVELLMPAMQAAELW
QESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRDEVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLYKAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKELEKVATPDQKAIEEVSAFLNIEADKCIKSMVFKVDEKLVVVLVRGDHEVNDVKVKNVYGASVVELASHE
EVKELLNCEVGSLGPIGVNGDIEIIADHAVASIVNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTI
LFARGIEVGHVFKLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATGESEEIKVEELQTY
IANILK

Sequences:

>Translated_566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREEMERAGAVELLMPAMQAAELW
QESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRDEVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLYKAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKELEKVATPDQKAIEEVSAFLNIEADKCIKSMVFKVDEKLVVVLVRGDHEVNDVKVKNVYGASVVELASHE
EVKELLNCEVGSLGPIGVNGDIEIIADHAVASIVNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTI
LFARGIEVGHVFKLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATGESEEIKVEELQTY
IANILK
>Mature_566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREEMERAGAVELLMPAMQAAELW
QESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRDEVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLYKAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKELEKVATPDQKAIEEVSAFLNIEADKCIKSMVFKVDEKLVVVLVRGDHEVNDVKVKNVYGASVVELASHE
EVKELLNCEVGSLGPIGVNGDIEIIADHAVASIVNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTI
LFARGIEVGHVFKLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATGESEEIKVEELQTY
IANILK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.9203539823009, Blast_Score=213, Evalue=4e-55,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=52.0069808027923, Blast_Score=601, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI115532348, Length=241, Percent_Identity=36.0995850622407, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=36.4705882352941, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=226, Percent_Identity=38.9380530973451, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24656200, Length=256, Percent_Identity=33.59375, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63193; Mature: 63193

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MERAGAVELLMPAMQAAELWQESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRD
HHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
EVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLYKA
HHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEECCEEE
TYTKSDEAEKELEKVATPDQKAIEEVSAFLNIEADKCIKSMVFKVDEKLVVVLVRGDHEV
EECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCC
NDVKVKNVYGASVVELASHEEVKELLNCEVGSLGPIGVNGDIEIIADHAVASIVNGCSGA
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCC
NEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTILFARGIEVGHVFKLGTRYSE
CCCCEEEEECCCCCCCEECCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCHHHH
AMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECC
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVE
CCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCEEECCCEECCEEEEEECCCCCCCEEE
VKVRATGESEEIKVEELQTYIANILK
EEEEECCCCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MERAGAVELLMPAMQAAELWQESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRD
HHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
EVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLYKA
HHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEECCEEE
TYTKSDEAEKELEKVATPDQKAIEEVSAFLNIEADKCIKSMVFKVDEKLVVVLVRGDHEV
EECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCC
NDVKVKNVYGASVVELASHEEVKELLNCEVGSLGPIGVNGDIEIIADHAVASIVNGCSGA
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCC
NEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTILFARGIEVGHVFKLGTRYSE
CCCCEEEEECCCCCCCEECCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCHHHH
AMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECC
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVE
CCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCEEECCCEECCEEEEEECCCCCCCEEE
VKVRATGESEEIKVEELQTYIANILK
EEEEECCCCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA